Cloned (Comment) | Organism |
---|---|
gene budC, phylogenetic tree, functional recombinant expression in Bacillus subtilis strain 168 | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Bacillus subtilis is engineered to produce chiral pure meso-2,3-BD. D-2,3-butanediol production is abolished by deleting D-2,3-butanediol dehydrogenase (EC 1.1.1.4) coding gene bdhA, and acoA gene is knocked out to prevent the degradation of acetoin, the immediate precursor of 2,3-butanediol. Next, both pta and ldh gene are deleted to decrease the accumulation of the byproducts, acetate and L-lactate. The meso-2,3-butanediol dehydrogenase coding gene from Klebsiella pneumoniae CICC10011 is introduced, as well as alsSD overexpressed in the tetra mutant (DELTAacoADELTAbdhADELTAptaDELTAldh) to achieve the efficient production of chiral meso-2,3-butanediol. Finally, the pool of NADH availability is further increased to facilitate the conversion of meso-2,3-butanediol from acetoin by overexpressing the udhA gene (coding a soluble transhydrogenase) and low dissolved oxygen control during the cultivation. Under microaerobic oxygen conditions, the best strain BSF9 produced 103.7 g/L meso-2,3-butanediol with a yield of 0.487 g/g glucose in the 5-L batch fermenter, and the titer of the main byproduct acetoin is no more than 1.1 g/L. Method optimization. The titer of meso-2,3-butanediol is almost unchanged at 37°C, 42°C, and 46°C, while the meso-2,3-butanediol productivity increases when the cultivation temperature is increased from 37°C to 46°C. The titer and productivity at 50°C decreases by 28.6% and 36.3% compared to those at 37°C | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | Klebsiella pneumoniae | - |
acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | Klebsiella pneumoniae CICC10011 | - |
acetoin + NADH + H+ | - |
r | |
acetoin + NADH + H+ | Klebsiella pneumoniae | - |
(2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
acetoin + NADH + H+ | Klebsiella pneumoniae CICC10011 | - |
(2R,3S)-butane-2,3-diol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae CICC10011 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae | acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | - |
Klebsiella pneumoniae CICC10011 | acetoin + NADH + H+ | - |
r | |
acetoin + NADH + H+ | - |
Klebsiella pneumoniae | (2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
acetoin + NADH + H+ | - |
Klebsiella pneumoniae CICC10011 | (2R,3S)-butane-2,3-diol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
budC | - |
Klebsiella pneumoniae |
meso-2,3-BD dehydrogenase | - |
Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Klebsiella pneumoniae | |
NADH | - |
Klebsiella pneumoniae |