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show all sequences of 1.1.1.B20

Cloning, expression and characterization of meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae

Zhang, G.L.; Wang, C.W.; Li, C.; Biotechnol. Lett. 34, 1519-1523 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene budC, expression in Escherichia coli strain BL21(DE3) pLys
Klebsiella pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NADH
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
0.65
-
(3R)-acetoin
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
0.66
-
NAD+
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
6
-
4-methyl-2-pentanone
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
13
-
meso-2,3-butanediol
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3R)-acetoin + NADH + H+
Klebsiella pneumoniae
-
meso-2,3-butanediol + NAD+
-
-
r
(3R)-acetoin + NADH + H+
Klebsiella pneumoniae XJ-Li
-
meso-2,3-butanediol + NAD+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
gene budC
-
Klebsiella pneumoniae XJ-Li
-
gene budC
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
21
-
purified reconstituted, recombinant enzyme, reduction of 4-methyl-2-pentanone, pH 8.0, 35C
Klebsiella pneumoniae
66
-
purified reconstituted, recombinant enzyme, oxidation of meso-2,3-butanediol, pH 8.0, 35C
Klebsiella pneumoniae
218
-
purified reconstituted, recombinant enzyme, reduction of acetoin, pH 8.0, 35C
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-acetoin + NADH + H+
-
721906
Klebsiella pneumoniae
meso-2,3-butanediol + NAD+
-
-
-
r
(3R)-acetoin + NADH + H+
-
721906
Klebsiella pneumoniae XJ-Li
meso-2,3-butanediol + NAD+
-
-
-
r
4-methyl-2-pentanone + NADH + H+
low activity
721906
Klebsiella pneumoniae
4-methyl-2-pentanol + NAD+
-
-
-
r
4-methyl-2-pentanone + NADH + H+
low activity
721906
Klebsiella pneumoniae XJ-Li
4-methyl-2-pentanol + NAD+
-
-
-
r
additional information
the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol
721906
Klebsiella pneumoniae
?
-
-
-
-
additional information
the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol
721906
Klebsiella pneumoniae XJ-Li
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Klebsiella pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Klebsiella pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Klebsiella pneumoniae
NADH
-
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene budC, expression in Escherichia coli strain BL21(DE3) pLys
Klebsiella pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Klebsiella pneumoniae
NADH
-
Klebsiella pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NADH
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
0.65
-
(3R)-acetoin
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
0.66
-
NAD+
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
6
-
4-methyl-2-pentanone
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
13
-
meso-2,3-butanediol
pH 8.0-9.0, 35C, recombinant enzyme
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3R)-acetoin + NADH + H+
Klebsiella pneumoniae
-
meso-2,3-butanediol + NAD+
-
-
r
(3R)-acetoin + NADH + H+
Klebsiella pneumoniae XJ-Li
-
meso-2,3-butanediol + NAD+
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
21
-
purified reconstituted, recombinant enzyme, reduction of 4-methyl-2-pentanone, pH 8.0, 35C
Klebsiella pneumoniae
66
-
purified reconstituted, recombinant enzyme, oxidation of meso-2,3-butanediol, pH 8.0, 35C
Klebsiella pneumoniae
218
-
purified reconstituted, recombinant enzyme, reduction of acetoin, pH 8.0, 35C
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-acetoin + NADH + H+
-
721906
Klebsiella pneumoniae
meso-2,3-butanediol + NAD+
-
-
-
r
(3R)-acetoin + NADH + H+
-
721906
Klebsiella pneumoniae XJ-Li
meso-2,3-butanediol + NAD+
-
-
-
r
4-methyl-2-pentanone + NADH + H+
low activity
721906
Klebsiella pneumoniae
4-methyl-2-pentanol + NAD+
-
-
-
r
4-methyl-2-pentanone + NADH + H+
low activity
721906
Klebsiella pneumoniae XJ-Li
4-methyl-2-pentanol + NAD+
-
-
-
r
additional information
the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol
721906
Klebsiella pneumoniae
?
-
-
-
-
additional information
the enzyme is highly stereospecific, and shows no significant activities towards 2R,3R-2,3-butanediol, 1,4-butanediol, and 2S,3S-2,3-butanediol
721906
Klebsiella pneumoniae XJ-Li
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
-
Klebsiella pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Klebsiella pneumoniae
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the short chain dehydrogenase/reductase family
Klebsiella pneumoniae
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the short chain dehydrogenase/reductase family
Klebsiella pneumoniae
Other publictions for EC 1.1.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721397
Zhang
A new NAD(H)-dependent meso-2, ...
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-
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6
4
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3
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5
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14
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4
2
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2
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1
2
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6
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4
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3
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-
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-
-
-
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14
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1
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4
2
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2
2
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-
721906
Zhang
Cloning, expression and charac ...
Klebsiella pneumoniae, Klebsiella pneumoniae XJ-Li
Biotechnol. Lett.
34
1519-1523
2012
-
-
1
-
-
-
-
5
-
-
-
2
-
5
-
-
-
-
-
-
3
-
6
-
1
-
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-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
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5
-
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-
2
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-
-
3
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6
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1
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1
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1
1
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702809
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Metabolic engineering of aceto ...
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Biotechnol. J.
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2010
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5
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-
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-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
710944
Gonzalez
Role of Saccharomyces cerevisi ...
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Appl. Environ. Microbiol.
76
670-679
2010
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-
-
-
-
-
-
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1
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1
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1
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1
1
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712019
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FEBS Lett.
584
219-223
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4
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1
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7
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2
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4
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1
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7
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2
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246405
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Crystal structure of meso-2,3- ...
Klebsiella pneumoniae
J. Biochem.
129
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2001
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1
1
2
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1
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-
-
-
-
-
1
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1
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1
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1
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1
-
1
2
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1
1
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1
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-
246408
Ui
Formation of a chiral acetoini ...
Klebsiella pneumoniae
Lett. Appl. Microbiol.
28
457-460
1999
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1
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1
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