BRENDA - Enzyme Database show
show all sequences of 1.1.1.B20

A new NAD(H)-dependent meso-2,3-butanediol dehydrogenase from an industrially potential strain Serratia marcescens H30

Zhang, L.; Xu, Q.; Zhan, S.; Li, Y.; Lin, H.; Sun, S.; Sha, L.; Hu, K.; Guan, X.; Shen, Y.; Appl. Microbiol. Biotechnol. 98, 1175-1184 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene budC, sequence comparisons, expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Serratia marcescens
Inhibitors
Inhibitors
Commentary
Organism
Structure
CaCl2
-
Serratia marcescens
Fe3+
inhibits diacetyl and (3S/3R)-acetoin reduction
Serratia marcescens
FeCl3
-
Serratia marcescens
NaCl
-
Serratia marcescens
NH4Cl
-
Serratia marcescens
ZnSO4
-
Serratia marcescens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.97
-
(3R)-acetoin
pH 8.0, 40°C
Serratia marcescens
3.3
-
diacetyl
pH 8.0, 40°C
Serratia marcescens
4.1
-
meso-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
31.2
-
(2S,3S)-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
enhances for oxidation and reduction reactions
Serratia marcescens
Mg2+
enhances diacetyl and (3S/3R)-acetoin reduction
Serratia marcescens
Mn2+
enhances diacetyl and (3S/3R)-acetoin reduction
Serratia marcescens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Serratia marcescens
H9XP47
gene budC
-
Serratia marcescens H30
H9XP47
gene budC
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2,3-butanediol + NAD+
low activity
721397
Serratia marcescens
(3S)-acetoin + NADH + H+
-
-
-
r
(2S,3S)-2,3-butanediol + NAD+
low activity
721397
Serratia marcescens H30
(3S)-acetoin + NADH + H+
-
-
-
r
(3R)-acetoin + NADH + H+
-
721397
Serratia marcescens
meso-2,3-butanediol + NAD+
-
-
-
r
(3R)-acetoin + NADH + H+
-
721397
Serratia marcescens H30
meso-2,3-butanediol + NAD+
-
-
-
r
(3S)-acetoin + NADH + H+
at pH 9.0
721397
Serratia marcescens
(2S,3S)-2,3-butanediol + NAD+
-
-
-
r
(3S)-acetoin + NADH + H+
at pH 9.0
721397
Serratia marcescens H30
(2S,3S)-2,3-butanediol + NAD+
-
-
-
r
1,2-pentanediol + NADH + H+
low activity
721397
Serratia marcescens
?
-
-
-
?
1,2-pentanediol + NADH + H+
low activity
721397
Serratia marcescens H30
?
-
-
-
?
1,2-propandiol + NADH + H+
low activity
721397
Serratia marcescens
?
-
-
-
?
diacetyl + NADH + H+
-
721397
Serratia marcescens
(3S)-acetoin + NAD+
-
-
-
?
glycerol + NADH + H+
low activity
721397
Serratia marcescens
?
-
-
-
?
meso-2,3-butanediol + NAD+
-
721397
Serratia marcescens
(3R)-acetoin + NADH + H+
-
-
-
r
additional information
(2R,3R)-2,3-butanediol is no substrate for the enzyme
721397
Serratia marcescens
?
-
-
-
-
additional information
(2R,3R)-2,3-butanediol is no substrate for the enzyme
721397
Serratia marcescens H30
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
optimum for oxidation and reduction reactions
Serratia marcescens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.02
-
(2S,3S)-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
6.2
-
meso-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
11.5
-
diacetyl
pH 8.0, 40°C
Serratia marcescens
19.7
-
(3R)-acetoin
pH 8.0, 40°C
Serratia marcescens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
maximal activity of (3S/3R)-acetoin reduction
Serratia marcescens
8
-
maximal activity of diacetyl reduction and meso-2,3-butanediol oxidation
Serratia marcescens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Serratia marcescens
NADH
-
Serratia marcescens
Cloned(Commentary) (protein specific)
Commentary
Organism
gene budC, sequence comparisons, expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
Serratia marcescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Serratia marcescens
NADH
-
Serratia marcescens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CaCl2
-
Serratia marcescens
Fe3+
inhibits diacetyl and (3S/3R)-acetoin reduction
Serratia marcescens
FeCl3
-
Serratia marcescens
NaCl
-
Serratia marcescens
NH4Cl
-
Serratia marcescens
ZnSO4
-
Serratia marcescens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.97
-
(3R)-acetoin
pH 8.0, 40°C
Serratia marcescens
3.3
-
diacetyl
pH 8.0, 40°C
Serratia marcescens
4.1
-
meso-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
31.2
-
(2S,3S)-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
enhances for oxidation and reduction reactions
Serratia marcescens
Mg2+
enhances diacetyl and (3S/3R)-acetoin reduction
Serratia marcescens
Mn2+
enhances diacetyl and (3S/3R)-acetoin reduction
Serratia marcescens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2,3-butanediol + NAD+
low activity
721397
Serratia marcescens
(3S)-acetoin + NADH + H+
-
-
-
r
(2S,3S)-2,3-butanediol + NAD+
low activity
721397
Serratia marcescens H30
(3S)-acetoin + NADH + H+
-
-
-
r
(3R)-acetoin + NADH + H+
-
721397
Serratia marcescens
meso-2,3-butanediol + NAD+
-
-
-
r
(3R)-acetoin + NADH + H+
-
721397
Serratia marcescens H30
meso-2,3-butanediol + NAD+
-
-
-
r
(3S)-acetoin + NADH + H+
at pH 9.0
721397
Serratia marcescens
(2S,3S)-2,3-butanediol + NAD+
-
-
-
r
(3S)-acetoin + NADH + H+
at pH 9.0
721397
Serratia marcescens H30
(2S,3S)-2,3-butanediol + NAD+
-
-
-
r
1,2-pentanediol + NADH + H+
low activity
721397
Serratia marcescens
?
-
-
-
?
1,2-pentanediol + NADH + H+
low activity
721397
Serratia marcescens H30
?
-
-
-
?
1,2-propandiol + NADH + H+
low activity
721397
Serratia marcescens
?
-
-
-
?
diacetyl + NADH + H+
-
721397
Serratia marcescens
(3S)-acetoin + NAD+
-
-
-
?
glycerol + NADH + H+
low activity
721397
Serratia marcescens
?
-
-
-
?
meso-2,3-butanediol + NAD+
-
721397
Serratia marcescens
(3R)-acetoin + NADH + H+
-
-
-
r
additional information
(2R,3R)-2,3-butanediol is no substrate for the enzyme
721397
Serratia marcescens
?
-
-
-
-
additional information
(2R,3R)-2,3-butanediol is no substrate for the enzyme
721397
Serratia marcescens H30
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
optimum for oxidation and reduction reactions
Serratia marcescens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.02
-
(2S,3S)-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
6.2
-
meso-2,3-butanediol
pH 5.0, 40°C
Serratia marcescens
11.5
-
diacetyl
pH 8.0, 40°C
Serratia marcescens
19.7
-
(3R)-acetoin
pH 8.0, 40°C
Serratia marcescens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
maximal activity of (3S/3R)-acetoin reduction
Serratia marcescens
8
-
maximal activity of diacetyl reduction and meso-2,3-butanediol oxidation
Serratia marcescens
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the shortchain dehydrogenase/reductase superfamily
Serratia marcescens
additional information
the enzyme possesses two conserved sequences including the coenzyme binding motif (GxxxGxG) and the active-site motif (YxxxK)
Serratia marcescens
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the shortchain dehydrogenase/reductase superfamily
Serratia marcescens
additional information
the enzyme possesses two conserved sequences including the coenzyme binding motif (GxxxGxG) and the active-site motif (YxxxK)
Serratia marcescens
Other publictions for EC 1.1.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721397
Zhang
A new NAD(H)-dependent meso-2, ...
Serratia marcescens, Serratia marcescens H30
Appl. Microbiol. Biotechnol.
98
1175-1184
2014
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-
1
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6
4
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3
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5
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14
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1
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4
2
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2
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1
2
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6
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4
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3
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14
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1
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4
2
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2
2
-
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-
721906
Zhang
Cloning, expression and charac ...
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Biotechnol. Lett.
34
1519-1523
2012
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-
1
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5
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2
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5
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3
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1
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1
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2
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1
2
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5
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3
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6
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1
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1
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1
1
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-
702809
Nielsen
Metabolic engineering of aceto ...
Escherichia coli, Saccharomyces cerevisiae
Biotechnol. J.
5
274-284
2010
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2
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1
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2
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5
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3
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2
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2
2
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1
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2
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3
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2
2
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710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
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1
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1
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1
1
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-
712019
Otagiri
Structural basis for chiral su ...
Corynebacterium glutamicum
FEBS Lett.
584
219-223
2010
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4
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1
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1
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7
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2
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4
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7
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2
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246405
Otagiri
Crystal structure of meso-2,3- ...
Klebsiella pneumoniae
J. Biochem.
129
205-208
2001
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1
1
2
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1
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1
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246408
Ui
Formation of a chiral acetoini ...
Klebsiella pneumoniae
Lett. Appl. Microbiol.
28
457-460
1999
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