BRENDA - Enzyme Database show
show all sequences of 1.1.1.B20

Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases

Otagiri, M.; Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Kurisu, G.; Kusunoki, M.; FEBS Lett. 584, 219-223 (2010)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
N146F
11% of wild-type specific activity
Corynebacterium glutamicum
Q140I
trace activity below 0.1 U/mg
Corynebacterium glutamicum
Q140I/N146F
poor activity
Corynebacterium glutamicum
Q140I/N146F/W190H
trace activity below 0.1 U/mg with substrate meso-butanediol, 2.9 U/mg with substrate (2S,3S)-2,3-butanediol
Corynebacterium glutamicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
meso-2,3-butanediol
pH 8.0, 37°C
Corynebacterium glutamicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1
-
mutant N146F, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
2.9
-
mutant Q140I/N146F/W190H, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
3.1
-
mutant Q140I/N146F, substrate (2,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
4.5
-
mutant Q140I/N146F, substrate meso-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
5.5
-
wild-type, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
19.4
-
mutant N146F, substrate meso-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
177
-
wild-type, substrate meso-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2,3-butanediol + NAD+
(2S,3S)-2,3-butanediol is a poor substrate
712019
Corynebacterium glutamicum
(3S)-acetoin + NADH
-
-
-
?
meso-2,3-butanediol + NAD+
-
712019
Corynebacterium glutamicum
acetoin + NADH
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
N146F
11% of wild-type specific activity
Corynebacterium glutamicum
Q140I
trace activity below 0.1 U/mg
Corynebacterium glutamicum
Q140I/N146F
poor activity
Corynebacterium glutamicum
Q140I/N146F/W190H
trace activity below 0.1 U/mg with substrate meso-butanediol, 2.9 U/mg with substrate (2S,3S)-2,3-butanediol
Corynebacterium glutamicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
-
meso-2,3-butanediol
pH 8.0, 37°C
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1
-
mutant N146F, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
2.9
-
mutant Q140I/N146F/W190H, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
3.1
-
mutant Q140I/N146F, substrate (2,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
4.5
-
mutant Q140I/N146F, substrate meso-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
5.5
-
wild-type, substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
19.4
-
mutant N146F, substrate meso-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
177
-
wild-type, substrate meso-2,3-butanediol, pH 8.0, 37°C
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2,3-butanediol + NAD+
(2S,3S)-2,3-butanediol is a poor substrate
712019
Corynebacterium glutamicum
(3S)-acetoin + NADH
-
-
-
?
meso-2,3-butanediol + NAD+
-
712019
Corynebacterium glutamicum
acetoin + NADH
-
-
-
?
Other publictions for EC 1.1.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721397
Zhang
A new NAD(H)-dependent meso-2, ...
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Appl. Microbiol. Biotechnol.
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14
-
1
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4
2
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2
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1
2
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6
-
4
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3
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-
-
14
-
1
-
-
4
2
-
-
-
-
2
2
-
-
-
721906
Zhang
Cloning, expression and charac ...
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Biotechnol. Lett.
34
1519-1523
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1
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5
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2
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5
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3
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6
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1
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1
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-
2
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1
2
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5
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2
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3
-
6
-
1
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-
1
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-
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1
1
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-
-
702809
Nielsen
Metabolic engineering of aceto ...
Escherichia coli, Saccharomyces cerevisiae
Biotechnol. J.
5
274-284
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-
2
-
1
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-
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2
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5
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3
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2
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2
2
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1
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2
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3
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2
2
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-
710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
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-
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1
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1
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1
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-
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-
1
1
-
-
-
712019
Otagiri
Structural basis for chiral su ...
Corynebacterium glutamicum
FEBS Lett.
584
219-223
2010
-
-
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
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-
-
7
-
2
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4
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1
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-
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7
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2
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-
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-
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-
246405
Otagiri
Crystal structure of meso-2,3- ...
Klebsiella pneumoniae
J. Biochem.
129
205-208
2001
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-
1
1
2
-
1
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-
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-
-
1
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1
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1
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1
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1
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1
2
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1
1
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1
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-
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246408
Ui
Formation of a chiral acetoini ...
Klebsiella pneumoniae
Lett. Appl. Microbiol.
28
457-460
1999
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1
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1
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1
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1
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1
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