BRENDA - Enzyme Database show
show all sequences of 1.1.1.B18

Enantioselective synthesis of (S)-phenylephrine by whole cells of recombinant Escherichia coli expressing the amino alcohol dehydrogenase gene from Rhodococcus erythropolis BCRC 10909

Lin, W.; Chen, C.; Chen, H.; Hsu, W.; Process Biochem. 45, 1529-1536 (2010)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
coversion of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone to (S)-phenylephrine with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7. The (S)-phenylepinephrine, recovered from reaction mixture by precipitation at pH 11.3, can be converted to (R)-phenylepinephrine by Walden inversion reaction
Rhodococcus erythropolis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26580
-
x * 26580, calcuulated, x * 30000, SDS-PAGE
Rhodococcus erythropolis
30000
-
x * 26580, calcuulated, x * 30000, SDS-PAGE
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
D6MMN3
-
-
Rhodococcus erythropolis BCRC 10909
D6MMN3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
713441
Rhodococcus erythropolis
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7
-
-
-
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
713441
Rhodococcus erythropolis BCRC 10909
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 26580, calcuulated, x * 30000, SDS-PAGE
Rhodococcus erythropolis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
specific for NADPH
Rhodococcus erythropolis
Application (protein specific)
Application
Commentary
Organism
synthesis
coversion of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone to (S)-phenylephrine with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7. The (S)-phenylepinephrine, recovered from reaction mixture by precipitation at pH 11.3, can be converted to (R)-phenylepinephrine by Walden inversion reaction
Rhodococcus erythropolis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
specific for NADPH
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26580
-
x * 26580, calcuulated, x * 30000, SDS-PAGE
Rhodococcus erythropolis
30000
-
x * 26580, calcuulated, x * 30000, SDS-PAGE
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
713441
Rhodococcus erythropolis
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7
-
-
-
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
713441
Rhodococcus erythropolis BCRC 10909
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 26580, calcuulated, x * 30000, SDS-PAGE
Rhodococcus erythropolis
Other publictions for EC 1.1.1.B18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724052
Urano
Genetic analysis around aminoa ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
89
739-746
2011
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-
1
-
1
-
-
-
-
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2
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4
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-
-
-
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2
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-
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-
-
-
-
-
2
-
-
-
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-
1
2
-
1
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-
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-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
713441
Lin
-
Enantioselective synthesis of ...
Rhodococcus erythropolis, Rhodococcus erythropolis BCRC 10909
Process Biochem.
45
1529-1536
2010
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
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-
1
-
1
-
-
-
-
-
-
-
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-
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
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-
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-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
712916
Kataoka
A novel NADP+-dependent L-1-am ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Lett. Appl. Microbiol.
43
430-455
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
1
-
10
1
2
-
-
-
1
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
10
1
2
-
-
-
1
-
-
-
1
-
-
1
-
-