Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.B18 extracted from

  • Lin, W.; Chen, C.; Chen, H.; Hsu, W.
    Enantioselective synthesis of (S)-phenylephrine by whole cells of recombinant Escherichia coli expressing the amino alcohol dehydrogenase gene from Rhodococcus erythropolis BCRC 10909 (2010), Process Biochem., 45, 1529-1536.
No PubMed abstract available

Application

Application Comment Organism
synthesis coversion of 1-(3-hydroxyphenyl)-2-(methylamino) ethanone to (S)-phenylephrine with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7. The (S)-phenylepinephrine, recovered from reaction mixture by precipitation at pH 11.3, can be converted to (R)-phenylepinephrine by Walden inversion reaction Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26580
-
x * 26580, calcuulated, x * 30000, SDS-PAGE Rhodococcus erythropolis
30000
-
x * 26580, calcuulated, x * 30000, SDS-PAGE Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis D6MMN3
-
-
Rhodococcus erythropolis BCRC 10909 D6MMN3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
Rhodococcus erythropolis (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7 ?
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
Rhodococcus erythropolis BCRC 10909 (1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+ conversion with with more than 99% enantiomeric excess, 78% yield and a productivity of 3.9 mmol(S)-phenylepinephrine/l h in 12 h at 30°C and pH 7 ?

Subunits

Subunits Comment Organism
? x * 26580, calcuulated, x * 30000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
amino alcohol dehydrogenase
-
Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
NADPH specific for NADPH Rhodococcus erythropolis