BRENDA - Enzyme Database show
show all sequences of 1.1.1.B18

A novel NADP+-dependent L-1-amino-2-propanol dehydrogenase from Rhodococcus erythropolis MAK154: a promising enzyme for the production of double chiral aminoalcohols

Kataoka, M.; Nakamura, Y.; Urano, N.; Ishige, T.; Shi, G.; Kita, S.; Sakamoto, K.; Shimizu, S.; Lett. Appl. Microbiol. 43, 430-455 (2006)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28500
-
4 * 28500, SDS-PAGE
Rhodococcus erythropolis
105000
-
gel filtration
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
A1IG83
-
-
Rhodococcus erythropolis MAK154
A1IG83
-
-
Purification (Commentary)
Commentary
Organism
-
Rhodococcus erythropolis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.43
-
pH 7.5, 43C
Rhodococcus erythropolis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
712916
Rhodococcus erythropolis
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
-
-
-
r
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
712916
Rhodococcus erythropolis MAK154
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
-
-
-
r
DL-1-amino-2-butanol + NADPH + H+
95% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis
? + NADP+
-
-
-
?
DL-1-amino-2-butanol + NADPH + H+
95% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis MAK154
? + NADP+
-
-
-
?
DL-2-aminocyclohexanol + NADPH + H+
50% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis
? + NADP+
-
-
-
?
DL-2-aminocyclohexanol + NADPH + H+
50% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis MAK154
? + NADP+
-
-
-
?
L-1-amino-2-propanol + NADPH + H+
best substrate
712916
Rhodococcus erythropolis
? + NADP+
-
-
-
?
L-1-amino-2-propanol + NADPH + H+
best substrate
712916
Rhodococcus erythropolis MAK154
? + NADP+
-
-
-
?
additional information
no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol
712916
Rhodococcus erythropolis
?
-
-
-
-
additional information
no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol
712916
Rhodococcus erythropolis MAK154
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 28500, SDS-PAGE
Rhodococcus erythropolis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
43
-
assay at
Rhodococcus erythropolis
55
-
-
Rhodococcus erythropolis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28500
-
4 * 28500, SDS-PAGE
Rhodococcus erythropolis
105000
-
gel filtration
Rhodococcus erythropolis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodococcus erythropolis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.43
-
pH 7.5, 43C
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
712916
Rhodococcus erythropolis
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
-
-
-
r
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
-
712916
Rhodococcus erythropolis MAK154
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
-
-
-
r
DL-1-amino-2-butanol + NADPH + H+
95% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis
? + NADP+
-
-
-
?
DL-1-amino-2-butanol + NADPH + H+
95% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis MAK154
? + NADP+
-
-
-
?
DL-2-aminocyclohexanol + NADPH + H+
50% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis
? + NADP+
-
-
-
?
DL-2-aminocyclohexanol + NADPH + H+
50% of the activity with L-1-amino-2-propanol
712916
Rhodococcus erythropolis MAK154
? + NADP+
-
-
-
?
L-1-amino-2-propanol + NADPH + H+
best substrate
712916
Rhodococcus erythropolis
? + NADP+
-
-
-
?
L-1-amino-2-propanol + NADPH + H+
best substrate
712916
Rhodococcus erythropolis MAK154
? + NADP+
-
-
-
?
additional information
no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol
712916
Rhodococcus erythropolis
?
-
-
-
-
additional information
no substrate: D-1-amino-2-propanol, 2-aminoethanol, 2-bromoethanol, 2-buten-1-ol, 2-propen-1-ol, 2-phenylethanol, 3-phenyl-1-propanol, 2,5-hexanediol, trimethyleneglycol, 1-nonanol, 1-decanol, 1-dodecanol, 2-butanol, 1,3-butanediol and 1,4-butanediol
712916
Rhodococcus erythropolis MAK154
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 28500, SDS-PAGE
Rhodococcus erythropolis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
43
-
assay at
Rhodococcus erythropolis
55
-
-
Rhodococcus erythropolis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8.5
-
Rhodococcus erythropolis
Expression
Organism
Commentary
Expression
Rhodococcus erythropolis
expression is induced on cultivation with aminoalcohols such as 1-amino-2-propanol, 1-amino-2-butanol and 2-aminocyclohexanol
up
Expression (protein specific)
Organism
Commentary
Expression
Rhodococcus erythropolis
expression is induced on cultivation with aminoalcohols such as 1-amino-2-propanol, 1-amino-2-butanol and 2-aminocyclohexanol
up
Other publictions for EC 1.1.1.B18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724052
Urano
Genetic analysis around aminoa ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
89
739-746
2011
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
713441
Lin
-
Enantioselective synthesis of ...
Rhodococcus erythropolis, Rhodococcus erythropolis BCRC 10909
Process Biochem.
45
1529-1536
2010
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
712916
Kataoka
A novel NADP+-dependent L-1-am ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Lett. Appl. Microbiol.
43
430-455
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
1
-
10
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
10
1
2
-
-
-
1
-
-
-
1
-
-
1
-
-