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show all sequences of 1.1.1.B18

Cloning and expression of the L-1-amino-2-propanol dehydrogenase gene from Rhodococcus erythropolis, and its application to double chiral compound production

Kataoka, M.; Ishige, T.; Urano, N.; Nakamura, Y.; Sakuradani, E.; Fukui, S.; Kita, S.; Sakamoto, K.; Shimizu, S.; Appl. Microbiol. Biotechnol. 80, 597-604 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one
Rhodococcus erythropolis
Inhibitors
Inhibitors
Commentary
Organism
Structure
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol
product inhibition above 20 mg/ml
Rhodococcus erythropolis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26580
-
x * 26580, calculated
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
A1IG83
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
diastereoselective reduction
695805
Rhodococcus erythropolis
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
i.e. d-psi-pseudoephedrine, molar reaction yields of 89.1% and more than 99% enantiomeric and diastereomeric excess
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 26580, calculated
Rhodococcus erythropolis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rhodococcus erythropolis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodococcus erythropolis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (2S)-2-(methylamino)-1-phenylpropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one
Rhodococcus erythropolis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol
product inhibition above 20 mg/ml
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26580
-
x * 26580, calculated
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-2-(methylamino)-1-phenylpropan-1-one + NADPH + H+
diastereoselective reduction
695805
Rhodococcus erythropolis
(1S,2S)-2-(methylamino)-1-phenylpropan-1-ol + NADP+
i.e. d-psi-pseudoephedrine, molar reaction yields of 89.1% and more than 99% enantiomeric and diastereomeric excess
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 26580, calculated
Rhodococcus erythropolis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rhodococcus erythropolis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodococcus erythropolis
Other publictions for EC 1.1.1.B18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724052
Urano
Genetic analysis around aminoa ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
89
739-746
2011
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-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
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-
-
1
2
-
1
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
713441
Lin
-
Enantioselective synthesis of ...
Rhodococcus erythropolis, Rhodococcus erythropolis BCRC 10909
Process Biochem.
45
1529-1536
2010
-
1
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
712916
Kataoka
A novel NADP+-dependent L-1-am ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Lett. Appl. Microbiol.
43
430-455
2006
-
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
1
-
-
-
1
-
10
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
10
1
2
-
-
-
1
-
-
-
1
-
-
1
-
-