BRENDA - Enzyme Database show
show all sequences of 1.1.1.98

Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases

Jones, J.M.; Morrell, J.C.; Gould, S.J.; J. Biol. Chem. 275, 12590-12597 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
three different genes, HAOX1, HAOX2, HAOX3, coding for 3 different 2-hydroxy acid oxidases, overexpression in Escherichia coli strain DH10B as His-tagged protein and in human skin fibroblasts GM5756-T cells as c-myc-tagged protein
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
additional information
overexpression of HAOX1, HAOX2, HAOX3 in Escherichia coli strain DH10B as His-tagged proteins and in human skin fibroblasts GM5756-T cells as c-myc-tagged proteins
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Homo sapiens
5777
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
three different forms: HAOX1, HAOX2, HAOX3
-
Purification (Commentary)
Commentary
Organism
His-tagged protein
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
HAOX2
Homo sapiens
-
liver
HAOX1 and HAOX2
Homo sapiens
-
pancreas
HAOX1 and, exclusively, HAOX3
Homo sapiens
-
Cloned(Commentary) (protein specific)
Commentary
Organism
three different genes, HAOX1, HAOX2, HAOX3, coding for 3 different 2-hydroxy acid oxidases, overexpression in Escherichia coli strain DH10B as His-tagged protein and in human skin fibroblasts GM5756-T cells as c-myc-tagged protein
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
overexpression of HAOX1, HAOX2, HAOX3 in Escherichia coli strain DH10B as His-tagged proteins and in human skin fibroblasts GM5756-T cells as c-myc-tagged proteins
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
peroxisome
-
Homo sapiens
5777
-
Purification (Commentary) (protein specific)
Commentary
Organism
His-tagged protein
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
HAOX2
Homo sapiens
-
liver
HAOX1 and HAOX2
Homo sapiens
-
pancreas
HAOX1 and, exclusively, HAOX3
Homo sapiens
-
Other publictions for EC 1.1.1.98
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287560
Jones
Identification and characteriz ...
Homo sapiens
J. Biol. Chem.
275
12590-12597
2000
-
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287559
Levis
2-Hydroxy fatty acid oxidases ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
38
470-477
1970
-
-
-
-
-
-
-
-
2
-
-
2
-
1
-
-
1
-
-
1
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
1
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-