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show all sequences of 1.1.1.97

Stabilization and purification of the secondary metabolism specific enzyme, m-hydroxybenzylalcohol dehydrogenase

Scott, R.E.; Lam, K.S.; Gaucher, G.M.; Can. J. Microbiol. 32, 167-175 (1986)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dithiothreitol
1.0 mM and 10 mM, 56% increase in activity, enzyme activity in cell extracts
Penicillium urticae
General Stability
General Stability
Organism
14 min half-life of crude enzyme at 30°C in 100 mM Tris-HCl, pH 7.6, half-life is increased to 77 and 80 min by 2 mM MnCl2 and FeCl3 respectively, 3 mM NADPH increase half-life to 146 min
Penicillium urticae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
4 * 36000, SDS-PAGE
Penicillium urticae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxybenzyl alcohol + NADP+
Penicillium urticae
-
3-hydroxybenzaldehyde + NADPH
-
Penicillium urticae
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Penicillium urticae
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, Bio-Gel A, 2',5'-ADP-agarose, DEAE-Sephacell, 2',5'-ADP-agarose
Penicillium urticae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
522
-
-
Penicillium urticae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxybenzyl alcohol + NADP+
-
287555
Penicillium urticae
3-hydroxybenzaldehyde + NADPH
-
287555
Penicillium urticae
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 36000, SDS-PAGE
Penicillium urticae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dithiothreitol
1.0 mM and 10 mM, 56% increase in activity, enzyme activity in cell extracts
Penicillium urticae
General Stability (protein specific)
General Stability
Organism
14 min half-life of crude enzyme at 30°C in 100 mM Tris-HCl, pH 7.6, half-life is increased to 77 and 80 min by 2 mM MnCl2 and FeCl3 respectively, 3 mM NADPH increase half-life to 146 min
Penicillium urticae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
4 * 36000, SDS-PAGE
Penicillium urticae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxybenzyl alcohol + NADP+
Penicillium urticae
-
3-hydroxybenzaldehyde + NADPH
-
Penicillium urticae
r
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, Bio-Gel A, 2',5'-ADP-agarose, DEAE-Sephacell, 2',5'-ADP-agarose
Penicillium urticae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
522
-
-
Penicillium urticae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxybenzyl alcohol + NADP+
-
287555
Penicillium urticae
3-hydroxybenzaldehyde + NADPH
-
287555
Penicillium urticae
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 36000, SDS-PAGE
Penicillium urticae
Other publictions for EC 1.1.1.97
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287555
Scott
Stabilization and purification ...
Penicillium urticae
Can. J. Microbiol.
32
167-175
1986
1
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1
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1
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1
1
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5672
Hopper
Regulation of enzymes of the 3 ...
Pseudomonas putida
J. Bacteriol.
142
21-26
1980
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1
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4
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1
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4
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1
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-
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287557
Gaucher
meta-Hydroxybenzyl-alcohol deh ...
Penicillium urticae
Methods Enzymol.
43
540-548
1975
-
-
-
-
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2
1
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1
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1
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1
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6
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1
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2
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1
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1
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1
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6
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1
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287558
Forrester
m-hydroxybenzyl alcohol dehydr ...
Penicillium urticae
Biochemistry
11
1108-1114
1972
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2
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1
1
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2
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1
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5
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1
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2
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1
1
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1
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5
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1
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