BRENDA - Enzyme Database show
show all sequences of 1.1.1.96

Isolation and properties of aromatic alpha-keto acid reductase

Zannoni, V.G.; Weber, W.W.; J. Biol. Chem. 241, 1340-1344 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,5-dihydroxybenzoic acid
1 mM, 44% inhibition
Bos taurus
2,5-dihydroxyphenyllactic acid
1 mM, 20% inhibition
Bos taurus
3,5-Diiodo-4-hydroxyphenyllactic acid
1 mM, 18% inhibition
Bos taurus
3,5-diiodotyrosine
1 mM, 15% inhibition
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
3,5-diiodo-4-hydroxyphenylpyruvate
-
Bos taurus
0.052
-
NADH
-
Bos taurus
1.9
-
4-hydroxyphenylpyruvate
-
Bos taurus
4.8
-
phenylpyruvate
-
Bos taurus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Canis lupus familiaris
-
-
-
Cavia porcellus
-
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate, calcium phosphate gel
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Cavia porcellus
-
blood
-
Cavia porcellus
-
brain
-
Canis lupus familiaris
-
brain
-
Cavia porcellus
-
heart
-
Bos taurus
-
heart
-
Canis lupus familiaris
-
heart
-
Cavia porcellus
-
kidney
-
Canis lupus familiaris
-
kidney
-
Cavia porcellus
-
liver
-
Canis lupus familiaris
-
liver
-
Cavia porcellus
-
muscle
-
Cavia porcellus
-
thyroid gland
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
41.75
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
5C, 160 mM phosphate, pH 6.5, 1 month, 20% activity lost
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,5-dinitro-4-hydroxyphenylpyruvate + NADH
-
287554
Bos taurus
3,5-dinitro-4-hydroxyphenyllactate + NAD+
-
287554
Bos taurus
r
3-(3,5-dibromo-4-hydroxyphenyl)pyruvate + NADH
-
287554
Bos taurus
3-(3,5-dibromo-4-hydroxyphenyl)lactate + NAD+
-
287554
Bos taurus
r
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
-
287554
Canis lupus familiaris
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
287554
Canis lupus familiaris
r
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
formation of phenyllactic acids is strongly favoured
287554
Bos taurus
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
287554
Bos taurus
r
3-bromo-4-hydroxyphenylpyruvate + NADH
-
287554
Bos taurus
3-bromo-4-hydroxyphenyllactate + NAD+
-
287554
Bos taurus
r
3-fluorophenylpyruvate + NADH
-
287554
Bos taurus
3-fluorophenyllactate + NAD+
-
287554
Bos taurus
r
4-hydroxyphenylpyruvate + NADH
-
287554
Bos taurus
4-hydroxyphenyllactate + NAD+
-
287554
Bos taurus
r
indolepyruvate + NADH
-
287554
Bos taurus
indolelactate + NAD+
-
287554
Bos taurus
r
indolepyruvate + NADH
-
287554
Canis lupus familiaris
indolelactate + NAD+
-
287554
Canis lupus familiaris
r
phenylpyruvate + NADH
-
287554
Bos taurus
phenyllactate + NAD+
-
287554
Bos taurus
r
phenylpyruvate + NADH
-
287554
Canis lupus familiaris
phenyllactate + NAD+
-
287554
Canis lupus familiaris
r
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
62
-
not completely inactivated after 2 min, completely inactivated above 72C
Bos taurus
72
-
complete inactivation in 2 min
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Bos taurus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Bos taurus
NADH
-
Canis lupus familiaris
NADPH
15% activity compared to NADH
Bos taurus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Bos taurus
NADH
-
Canis lupus familiaris
NADPH
15% activity compared to NADH
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,5-dihydroxybenzoic acid
1 mM, 44% inhibition
Bos taurus
2,5-dihydroxyphenyllactic acid
1 mM, 20% inhibition
Bos taurus
3,5-Diiodo-4-hydroxyphenyllactic acid
1 mM, 18% inhibition
Bos taurus
3,5-diiodotyrosine
1 mM, 15% inhibition
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.043
-
3,5-diiodo-4-hydroxyphenylpyruvate
-
Bos taurus
0.052
-
NADH
-
Bos taurus
1.9
-
4-hydroxyphenylpyruvate
-
Bos taurus
4.8
-
phenylpyruvate
-
Bos taurus
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate, calcium phosphate gel
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adrenal gland
-
Cavia porcellus
-
blood
-
Cavia porcellus
-
brain
-
Canis lupus familiaris
-
brain
-
Cavia porcellus
-
heart
-
Bos taurus
-
heart
-
Canis lupus familiaris
-
heart
-
Cavia porcellus
-
kidney
-
Canis lupus familiaris
-
kidney
-
Cavia porcellus
-
liver
-
Canis lupus familiaris
-
liver
-
Cavia porcellus
-
muscle
-
Cavia porcellus
-
thyroid gland
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
41.75
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
5C, 160 mM phosphate, pH 6.5, 1 month, 20% activity lost
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,5-dinitro-4-hydroxyphenylpyruvate + NADH
-
287554
Bos taurus
3,5-dinitro-4-hydroxyphenyllactate + NAD+
-
287554
Bos taurus
r
3-(3,5-dibromo-4-hydroxyphenyl)pyruvate + NADH
-
287554
Bos taurus
3-(3,5-dibromo-4-hydroxyphenyl)lactate + NAD+
-
287554
Bos taurus
r
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
-
287554
Canis lupus familiaris
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
287554
Canis lupus familiaris
r
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
formation of phenyllactic acids is strongly favoured
287554
Bos taurus
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
287554
Bos taurus
r
3-bromo-4-hydroxyphenylpyruvate + NADH
-
287554
Bos taurus
3-bromo-4-hydroxyphenyllactate + NAD+
-
287554
Bos taurus
r
3-fluorophenylpyruvate + NADH
-
287554
Bos taurus
3-fluorophenyllactate + NAD+
-
287554
Bos taurus
r
4-hydroxyphenylpyruvate + NADH
-
287554
Bos taurus
4-hydroxyphenyllactate + NAD+
-
287554
Bos taurus
r
indolepyruvate + NADH
-
287554
Bos taurus
indolelactate + NAD+
-
287554
Bos taurus
r
indolepyruvate + NADH
-
287554
Canis lupus familiaris
indolelactate + NAD+
-
287554
Canis lupus familiaris
r
phenylpyruvate + NADH
-
287554
Bos taurus
phenyllactate + NAD+
-
287554
Bos taurus
r
phenylpyruvate + NADH
-
287554
Canis lupus familiaris
phenyllactate + NAD+
-
287554
Canis lupus familiaris
r
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
62
-
not completely inactivated after 2 min, completely inactivated above 72C
Bos taurus
72
-
complete inactivation in 2 min
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Bos taurus
Other publictions for EC 1.1.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287552
Donald
A description of human aromati ...
Canis lupus familiaris, Homo sapiens
Ann. Hum. Genet.
46
299-306
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
15
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287553
Takada
Purification, characterization ...
Canis lupus familiaris
Life Sci.
20
609-616
1977
-
-
-
-
-
-
1
5
1
-
2
-
-
1
-
-
1
-
-
4
1
2
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
1
-
2
-
-
-
-
1
-
4
1
2
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287554
Zannoni
Isolation and properties of ar ...
Bos taurus, Canis lupus familiaris, Cavia porcellus
J. Biol. Chem.
241
1340-1344
1966
-
-
-
-
-
-
4
4
-
-
-
-
-
3
-
-
1
-
-
13
1
1
11
-
-
-
2
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
4
-
4
-
-
-
-
-
-
-
1
-
13
1
1
11
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-