BRENDA - Enzyme Database show
show all sequences of 1.1.1.96

Purification, characterization and identification of aromatic 2-oxo acid reductase

Takada, Y.; Noguchi, T.; Kido, R.; Life Sci. 20, 609-616 (1977)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,5-dihydroxybenzoic acid
0.5 mM, 60% inhibition
Canis lupus familiaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
NADH
-
Canis lupus familiaris
0.04
-
3,5-diiodo-4-hydroxyphenylpyruvate
-
Canis lupus familiaris
0.14
-
oxaloacetate
-
Canis lupus familiaris
0.2
-
Indolepyruvate
-
Canis lupus familiaris
1.6
-
phenylpyruvate
-
Canis lupus familiaris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Canis lupus familiaris
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Canis lupus familiaris
75000
-
sucrose-density gradient centrifugation
Canis lupus familiaris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Canis lupus familiaris
-
-
-
Purification (Commentary)
Commentary
Organism
DEAE-cellulose, hydroxyapatite, isoelectric focusing, Sephadex G-150
Canis lupus familiaris
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Canis lupus familiaris
-
heart
-
Canis lupus familiaris
-
kidney
-
Canis lupus familiaris
-
liver
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1200
-
-
Canis lupus familiaris
Storage Stability
Storage Stability
Organism
-20C, 50 mM potassium phosphate, pH 7.5, 2 months, no loss of activity
Canis lupus familiaris
4C, 50 mM potassium phosphate, pH 7.5, 2 weeks, no loss of activity
Canis lupus familiaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
-
287553
Canis lupus familiaris
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
287553
Canis lupus familiaris
r
indolepyruvate + NADH
-
287553
Canis lupus familiaris
indolelactate + NAD+
-
287553
Canis lupus familiaris
r
oxaloacetate + NADH
order of activity, oxaloacetate, 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate, indolepyruvate, phenylpyruvate
287553
Canis lupus familiaris
malate + NAD+
-
287553
Canis lupus familiaris
r
phenylpyruvate + NADH
-
287553
Canis lupus familiaris
phenyllactate + NAD+
-
287553
Canis lupus familiaris
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Canis lupus familiaris
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Canis lupus familiaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Canis lupus familiaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,5-dihydroxybenzoic acid
0.5 mM, 60% inhibition
Canis lupus familiaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
NADH
-
Canis lupus familiaris
0.04
-
3,5-diiodo-4-hydroxyphenylpyruvate
-
Canis lupus familiaris
0.14
-
oxaloacetate
-
Canis lupus familiaris
0.2
-
Indolepyruvate
-
Canis lupus familiaris
1.6
-
phenylpyruvate
-
Canis lupus familiaris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Canis lupus familiaris
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Canis lupus familiaris
75000
-
sucrose-density gradient centrifugation
Canis lupus familiaris
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-cellulose, hydroxyapatite, isoelectric focusing, Sephadex G-150
Canis lupus familiaris
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Canis lupus familiaris
-
heart
-
Canis lupus familiaris
-
kidney
-
Canis lupus familiaris
-
liver
-
Canis lupus familiaris
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
1200
-
-
Canis lupus familiaris
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 50 mM potassium phosphate, pH 7.5, 2 months, no loss of activity
Canis lupus familiaris
4C, 50 mM potassium phosphate, pH 7.5, 2 weeks, no loss of activity
Canis lupus familiaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-(3,5-diiodo-4-hydroxyphenyl)pyruvate + NADH
-
287553
Canis lupus familiaris
3-(3,5-diiodo-4-hydroxyphenyl)lactate + NAD+
-
287553
Canis lupus familiaris
r
indolepyruvate + NADH
-
287553
Canis lupus familiaris
indolelactate + NAD+
-
287553
Canis lupus familiaris
r
oxaloacetate + NADH
order of activity, oxaloacetate, 3-(3,5-diiodo-4-hydroxyphenyl)pyruvate, indolepyruvate, phenylpyruvate
287553
Canis lupus familiaris
malate + NAD+
-
287553
Canis lupus familiaris
r
phenylpyruvate + NADH
-
287553
Canis lupus familiaris
phenyllactate + NAD+
-
287553
Canis lupus familiaris
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 40000, SDS-PAGE
Canis lupus familiaris
Other publictions for EC 1.1.1.96
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287552
Donald
A description of human aromati ...
Canis lupus familiaris, Homo sapiens
Ann. Hum. Genet.
46
299-306
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
15
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
15
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287553
Takada
Purification, characterization ...
Canis lupus familiaris
Life Sci.
20
609-616
1977
-
-
-
-
-
-
1
5
1
-
2
-
-
1
-
-
1
-
-
4
1
2
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
5
1
-
2
-
-
-
-
1
-
4
1
2
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287554
Zannoni
Isolation and properties of ar ...
Bos taurus, Canis lupus familiaris, Cavia porcellus
J. Biol. Chem.
241
1340-1344
1966
-
-
-
-
-
-
4
4
-
-
-
-
-
3
-
-
1
-
-
13
1
1
11
-
-
-
2
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
4
-
4
-
-
-
-
-
-
-
1
-
13
1
1
11
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-