BRENDA - Enzyme Database show
show all sequences of 1.1.1.94

Rickettsia prowazekii uses an sn-glycerol-3-phosphate dehydrogenase and a novel dihydroxyacetone phosphate transport system to supply triose phosphate for phospholipid biosynthesis

Frohlich, K.M.; Roberts, R.A.; Housley, N.A.; Audia, J.P.; J. Bacteriol. 192, 4281-4288 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Rickettsia prowazekii
Engineering
Amino acid exchange
Commentary
Organism
additional information
heterologous expression in Escherichia coli complements an Escherichia coli gpsA mutant
Rickettsia prowazekii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.048
-
NADPH
pH 7.4, temperature not specified in the publication
Rickettsia prowazekii
0.528
-
glycerone phosphate
cosubstrate NADPH, pH 7.4, temperature not specified in the publication
Rickettsia prowazekii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36600
-
x * 36600, SDS-PAGE and calculated for His-tagged protein
Rickettsia prowazekii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rickettsia prowazekii
Q9ZDA0
-
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Rickettsia prowazekii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + NADH + H+
-
712303
Rickettsia prowazekii
sn-glycerol 3-phosphate + NAD+
-
-
-
r
glycerone phosphate + NADPH + H+
favoured reaction direction
712303
Rickettsia prowazekii
sn-glycerol 3-phosphate + NADP+
-
-
-
r
sn-glycerol 3-phosphate + NADP+
-
712303
Rickettsia prowazekii
glycerone phosphate + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
?
x * 36600, SDS-PAGE and calculated for His-tagged protein
Rickettsia prowazekii
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADPH is preferred over NADH as the cofactor
Rickettsia prowazekii
NADPH
NADPH is preferred over NADH as the cofactor
Rickettsia prowazekii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Rickettsia prowazekii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADPH is preferred over NADH as the cofactor
Rickettsia prowazekii
NADPH
NADPH is preferred over NADH as the cofactor
Rickettsia prowazekii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
heterologous expression in Escherichia coli complements an Escherichia coli gpsA mutant
Rickettsia prowazekii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.048
-
NADPH
pH 7.4, temperature not specified in the publication
Rickettsia prowazekii
0.528
-
glycerone phosphate
cosubstrate NADPH, pH 7.4, temperature not specified in the publication
Rickettsia prowazekii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36600
-
x * 36600, SDS-PAGE and calculated for His-tagged protein
Rickettsia prowazekii
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Rickettsia prowazekii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + NADH + H+
-
712303
Rickettsia prowazekii
sn-glycerol 3-phosphate + NAD+
-
-
-
r
glycerone phosphate + NADPH + H+
favoured reaction direction
712303
Rickettsia prowazekii
sn-glycerol 3-phosphate + NADP+
-
-
-
r
sn-glycerol 3-phosphate + NADP+
-
712303
Rickettsia prowazekii
glycerone phosphate + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36600, SDS-PAGE and calculated for His-tagged protein
Rickettsia prowazekii
General Information
General Information
Commentary
Organism
physiological function
Rickettsia prowazekii is unable to synthesize glycerone phosphate as a substrate for the GpsA enzymatic reaction. Purified Rickettsia prowazekii transports and incorporates glycerone phosphate into phospholipids, implicating a role for GpsA in vivo as part of a rickettsial G3P acquisition pathway for phospholipid biosynthesis
Rickettsia prowazekii
General Information (protein specific)
General Information
Commentary
Organism
physiological function
Rickettsia prowazekii is unable to synthesize glycerone phosphate as a substrate for the GpsA enzymatic reaction. Purified Rickettsia prowazekii transports and incorporates glycerone phosphate into phospholipids, implicating a role for GpsA in vivo as part of a rickettsial G3P acquisition pathway for phospholipid biosynthesis
Rickettsia prowazekii
Other publictions for EC 1.1.1.94
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738799
Lakshmanan
In silico model-driven cofacto ...
Escherichia coli
J. Ind. Microbiol. Biotechnol.
42
1401-1414
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
712303
Frohlich
Rickettsia prowazekii uses an ...
Rickettsia prowazekii
J. Bacteriol.
192
4281-4288
2010
-
-
1
-
1
-
-
2
-
-
1
-
-
4
-
-
1
-
-
-
-
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
2
-
-
1
-
-
-
-
1
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712449
Shen
Metabolic and transcriptional ...
Escherichia coli
J. Biol. Chem.
285
22957-22965
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
689802
Yeh
Structure of glycerol-3-phosph ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
105
3280-3285
2008
-
-
-
1
-
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690210
Watanabe
Expression of glycerol 3-phosp ...
Candida versatilis
Yeast
25
107-116
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
656732
Ruijter
Polyol accumulation by Aspergi ...
Aspergillus oryzae
Microbiology
150
1095-1101
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669177
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
255
3492-3497
1980
-
-
-
-
1
-
11
5
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
5
16
-
-
-
-
-
5
-
1
-
-
11
16
5
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
669174
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli K-12
J. Biol. Chem.
253
6348-6353
1978
-
-
-
-
1
-
1
-
-
-
7
1
-
1
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
-
4
-
1
-
-
-
-
4
-
1
-
-
1
-
-
-
-
7
1
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
669175
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
253
6354-6363
1978
-
-
-
-
1
-
4
1
-
-
-
1
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
4
3
-
-
-
-
-
4
-
1
-
-
4
3
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287523
Kito
Purification and regulatory pr ...
Escherichia coli
J. Biol. Chem.
244
3316-3323
1969
-
-
-
-
-
-
11
3
-
-
-
1
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
11
-
3
-
-
-
1
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287524
Kim
Properties of the nicotinamide ...
Oryctolagus cuniculus
J. Biol. Chem.
243
3351-3356
1968
-
-
-
-
-
-
16
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-