BRENDA - Enzyme Database show
show all sequences of 1.1.1.94

Expression of glycerol 3-phosphate dehydrogenase gene (CvGPD1) in salt-tolerant yeast Candida versatilis is stimulated by high concentrations of NaCl

Watanabe, Y.; Nagayama, K.; Tamai, Y.; Yeast 25, 107-116 (2008)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
glycerol 3-phosphate dehydrogenase gene (CvGPD1) from salt-tolerant yeast Candida versatilisis is expressed in glycerol synthesis-deficient Saccharomyces cerevisiae cells, the salt tolerance of the recombinant strain is enhanced, and NADP+-dependent GPDH, Cvgpd1p synthesis and recovery of glycerol synthesis are confirmed. The transcription of CvGPD1 in Candida versatilis cells is stimulated by high concentrations of NaCl
Candida versatilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida versatilis
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol 3-phosphate + NAD(P)+
-
690210
Candida versatilis
glycerone phosphate + NAD(P)H + H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Candida versatilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
glycerol 3-phosphate dehydrogenase gene (CvGPD1) from salt-tolerant yeast Candida versatilisis is expressed in glycerol synthesis-deficient Saccharomyces cerevisiae cells, the salt tolerance of the recombinant strain is enhanced, and NADP+-dependent GPDH, Cvgpd1p synthesis and recovery of glycerol synthesis are confirmed. The transcription of CvGPD1 in Candida versatilis cells is stimulated by high concentrations of NaCl
Candida versatilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol 3-phosphate + NAD(P)+
-
690210
Candida versatilis
glycerone phosphate + NAD(P)H + H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Candida versatilis
Other publictions for EC 1.1.1.94
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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689802
Yeh
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Proc. Natl. Acad. Sci. USA
105
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1
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Expression of glycerol 3-phosp ...
Candida versatilis
Yeast
25
107-116
2008
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Aspergillus oryzae
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150
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2004
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669177
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
255
3492-3497
1980
-
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1
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11
5
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1
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2
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16
5
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1
-
-
-
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-
-
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669174
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli K-12
J. Biol. Chem.
253
6348-6353
1978
-
-
-
-
1
-
1
-
-
-
7
1
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1
-
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669175
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
253
6354-6363
1978
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-
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-
1
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4
1
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287523
Kito
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Escherichia coli
J. Biol. Chem.
244
3316-3323
1969
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-
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-
-
11
3
-
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-
1
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1
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287524
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Oryctolagus cuniculus
J. Biol. Chem.
243
3351-3356
1968
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-
-
-
16
-
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1
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