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show all sequences of 1.1.1.94

Biosynthesis in Escherichia coli of sn-glycerol-3-phosphate, a precursor of phospholipid. Further kinetic characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase

Edgar, J.R.; Bell, R.M.; J. Biol. Chem. 255, 3492-3497 (1980)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
existence of a naturally occurring feedback inhibition-resistant mutant
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
2',5'-ADP
-
Escherichia coli
3,4-dihydroxybutyl 1-phosphonate
competitive versus dihydroxyacetone phosphate, binding to a regulatory site
Escherichia coli
ADP
-
Escherichia coli
ADP-ribose
-
Escherichia coli
AMP
-
Escherichia coli
ATP
-
Escherichia coli
ethylene glycol phosphate
binding to the active site
Escherichia coli
NAD+
-
Escherichia coli
NADP+
-
Escherichia coli
NMN
-
Escherichia coli
sn-glycerol-3-phosphate
the wild-type enzyme is feedback regulated, competitive versus dihydroxyacetone phosphate, binding to a regulatory site
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0037
-
NADPH
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.004
-
NADH
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.0041
-
NADPH
pH 7.4, 23C, wild-type mutant enzyme
Escherichia coli
0.0045
-
NADH
pH 7.4, 23C, wild-type mutant enzyme
Escherichia coli
0.005
-
reduced nicotinamide hypoxanthine dinucleotide
pH 7.4, 23C, wild-type mutant enzyme and feedback-resistant mutant enzyme
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerone phosphate + NAD(P)H
Escherichia coli
-
sn-glycerol-3-phosphate + NAD(P)+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + NAD(P)H
-
669177
Escherichia coli
sn-glycerol-3-phosphate + NAD(P)+
-
-
-
r
glycerone phosphate + NAD(P)H
i.e. dihydroxyacetone phosphate
669177
Escherichia coli
sn-glycerol-3-phosphate + NAD(P)+
product analysis
-
-
r
glycerone phosphate + NADH + H+
-
669177
Escherichia coli
sn-glycerol 3-phosphate + NAD+
-
-
-
-
glycerone phosphate + reduced nicotinamide hypoxanthine dinucleotide
-
669177
Escherichia coli
sn-glycerol-3-phosphate + oxidized nicotinamide hypoxanthine dinucleotide
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
23
-
assay at
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADP+
B-type specificity of wild-type and feedback-resistant mutant enzyme
Escherichia coli
NADPH
B-type specificity of wild-type and feedback-resistant mutant enzyme
Escherichia coli
nicotinamide hypoxanthine dinucleotide
-
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
product inhibition kinetics
Escherichia coli
0.1
-
ADP-ribose
pH 7.4, 23C, wild-type enzyme
Escherichia coli
0.19
-
NADP+
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.2
-
NAD+
pH 7.4, 23C, wild-type enzyme
Escherichia coli
0.21
-
ADP-ribose
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.22
-
NAD+
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.25
-
NADP+
pH 7.4, 23C, wild-type enzyme
Escherichia coli
0.7
-
ADP
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.8
-
ADP
pH 7.4, 23C, wild-type enzyme
Escherichia coli
1.4
-
ATP
pH 7.4, 23C, wild-type enzyme and feedback-resistant mutant enzyme
Escherichia coli
1.4
-
ethylene glycol phosphate
pH 7.4, 23C, wild-type and feedback-resistant mutant enzyme
Escherichia coli
1.8
-
2',5'-ADP
pH 7.4, 23C, wild-type enzyme
Escherichia coli
2
-
2',5'-ADP
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
4.8
-
AMP
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
5
-
AMP
pH 7.4, 23C, wild-type enzyme
Escherichia coli
10
-
NMN
pH 7.4, 23C, wild-type enzyme and feedback-resistant mutant enzyme
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADP+
B-type specificity of wild-type and feedback-resistant mutant enzyme
Escherichia coli
NADPH
B-type specificity of wild-type and feedback-resistant mutant enzyme
Escherichia coli
nicotinamide hypoxanthine dinucleotide
-
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
existence of a naturally occurring feedback inhibition-resistant mutant
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2',5'-ADP
-
Escherichia coli
3,4-dihydroxybutyl 1-phosphonate
competitive versus dihydroxyacetone phosphate, binding to a regulatory site
Escherichia coli
ADP
-
Escherichia coli
ADP-ribose
-
Escherichia coli
AMP
-
Escherichia coli
ATP
-
Escherichia coli
ethylene glycol phosphate
binding to the active site
Escherichia coli
NAD+
-
Escherichia coli
NADP+
-
Escherichia coli
NMN
-
Escherichia coli
sn-glycerol-3-phosphate
the wild-type enzyme is feedback regulated, competitive versus dihydroxyacetone phosphate, binding to a regulatory site
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
product inhibition kinetics
Escherichia coli
0.1
-
ADP-ribose
pH 7.4, 23C, wild-type enzyme
Escherichia coli
0.19
-
NADP+
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.2
-
NAD+
pH 7.4, 23C, wild-type enzyme
Escherichia coli
0.21
-
ADP-ribose
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.22
-
NAD+
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.25
-
NADP+
pH 7.4, 23C, wild-type enzyme
Escherichia coli
0.7
-
ADP
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.8
-
ADP
pH 7.4, 23C, wild-type enzyme
Escherichia coli
1.4
-
ATP
pH 7.4, 23C, wild-type enzyme and feedback-resistant mutant enzyme
Escherichia coli
1.4
-
ethylene glycol phosphate
pH 7.4, 23C, wild-type and feedback-resistant mutant enzyme
Escherichia coli
1.8
-
2',5'-ADP
pH 7.4, 23C, wild-type enzyme
Escherichia coli
2
-
2',5'-ADP
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
4.8
-
AMP
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
5
-
AMP
pH 7.4, 23C, wild-type enzyme
Escherichia coli
10
-
NMN
pH 7.4, 23C, wild-type enzyme and feedback-resistant mutant enzyme
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0037
-
NADPH
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.004
-
NADH
pH 7.4, 23C, feedback-resistant mutant enzyme
Escherichia coli
0.0041
-
NADPH
pH 7.4, 23C, wild-type mutant enzyme
Escherichia coli
0.0045
-
NADH
pH 7.4, 23C, wild-type mutant enzyme
Escherichia coli
0.005
-
reduced nicotinamide hypoxanthine dinucleotide
pH 7.4, 23C, wild-type mutant enzyme and feedback-resistant mutant enzyme
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerone phosphate + NAD(P)H
Escherichia coli
-
sn-glycerol-3-phosphate + NAD(P)+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + NAD(P)H
-
669177
Escherichia coli
sn-glycerol-3-phosphate + NAD(P)+
-
-
-
r
glycerone phosphate + NAD(P)H
i.e. dihydroxyacetone phosphate
669177
Escherichia coli
sn-glycerol-3-phosphate + NAD(P)+
product analysis
-
-
r
glycerone phosphate + NADH + H+
-
669177
Escherichia coli
sn-glycerol 3-phosphate + NAD+
-
-
-
-
glycerone phosphate + reduced nicotinamide hypoxanthine dinucleotide
-
669177
Escherichia coli
sn-glycerol-3-phosphate + oxidized nicotinamide hypoxanthine dinucleotide
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
23
-
assay at
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
Other publictions for EC 1.1.1.94
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738799
Lakshmanan
In silico model-driven cofacto ...
Escherichia coli
J. Ind. Microbiol. Biotechnol.
42
1401-1414
2015
-
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1
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1
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1
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1
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1
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1
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1
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712303
Frohlich
Rickettsia prowazekii uses an ...
Rickettsia prowazekii
J. Bacteriol.
192
4281-4288
2010
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1
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1
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2
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1
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4
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1
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3
1
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2
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1
2
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1
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2
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1
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1
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3
1
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1
1
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712449
Shen
Metabolic and transcriptional ...
Escherichia coli
J. Biol. Chem.
285
22957-22965
2010
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1
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1
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1
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-
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-
-
1
1
-
-
-
689802
Yeh
Structure of glycerol-3-phosph ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
105
3280-3285
2008
-
-
-
1
-
-
3
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
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-
1
1
-
-
-
3
-
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-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690210
Watanabe
Expression of glycerol 3-phosp ...
Candida versatilis
Yeast
25
107-116
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
1
-
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-
-
1
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
656732
Ruijter
Polyol accumulation by Aspergi ...
Aspergillus oryzae
Microbiology
150
1095-1101
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
1
4
-
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-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669177
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
255
3492-3497
1980
-
-
-
-
1
-
11
5
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
5
16
-
-
-
-
-
5
-
1
-
-
11
16
5
-
-
-
1
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
669174
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli K-12
J. Biol. Chem.
253
6348-6353
1978
-
-
-
-
1
-
1
-
-
-
7
1
-
1
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
-
4
-
1
-
-
-
-
4
-
1
-
-
1
-
-
-
-
7
1
-
-
-
1
-
-
1
1
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
669175
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
253
6354-6363
1978
-
-
-
-
1
-
4
1
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-
-
1
-
2
-
-
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1
-
-
-
-
2
-
1
-
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-
1
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-
4
3
-
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-
4
-
1
-
-
4
3
1
-
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-
1
-
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-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287523
Kito
Purification and regulatory pr ...
Escherichia coli
J. Biol. Chem.
244
3316-3323
1969
-
-
-
-
-
-
11
3
-
-
-
1
-
1
-
-
1
-
-
-
1
-
1
-
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-
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-
1
-
-
3
-
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-
3
-
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-
11
-
3
-
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1
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1
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-
1
-
1
-
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-
1
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-
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-
-
287524
Kim
Properties of the nicotinamide ...
Oryctolagus cuniculus
J. Biol. Chem.
243
3351-3356
1968
-
-
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-
16
-
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1
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1
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1
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16
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