Any feedback?
Literature summary for 1.1.1.94 extracted from
- Biosynthesis in Escherichia coli of sn-glycerol 3-phosphate, a precursor of phospholipid. Purification and physical characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase (1978), J. Biol. Chem., 253, 6348-6353.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | existence of a naturally occurring feedback inhibition-resistant mutant in strain BB26-36-R2 | Escherichia coli K-12 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| sn-glycerol-3-phosphate | the wild-type enzyme is feedback inhibited | Escherichia coli K-12 |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25900 | - |
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
| 26000 | - |
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
| 32500 | - |
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
| 48300 | - |
feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
| 49000 | - |
wild-type enzyme, gel filtration | Escherichia coli K-12 |
| 50000 | - |
feedback inhibition-resistant mutant, gel filtration | Escherichia coli K-12 |
| 50700 | - |
wild-type enzyme, sedimentation equilibrium analysis | Escherichia coli K-12 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate + NAD(P)H | Escherichia coli K-12 | the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview | sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | P0A6S7 | and strain BB26-36-R2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native wild-type enzyme from Escherichia coli K12 12000fold by streptomycin and ammonum sulfate fractionation, adsorption, hydroxy apatite, and anion exchange chromatography, and gel filtration to over 95% purity, feedback inhibition-resistant mutant from strain BB26-36-R2 | Escherichia coli K-12 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
purified wild-type enzyme | Escherichia coli K-12 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified concentrated wild-type enzyme, 50% glycerol, 25 mM Tris-HCl, pH 7.4, 0.5 mM DTT, 0.2 M NaCl, 5 mM glycerol-3-phosphate, 1 month, over 90% remaining activity | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerone phosphate + NAD(P)H | i.e. dihydroxyacetone phosphate | Escherichia coli K-12 | sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
| glycerone phosphate + NAD(P)H | the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview | Escherichia coli K-12 | sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis | Escherichia coli K-12 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sn-glycerol-3-phosphate dehydrogenase | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Escherichia coli K-12 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli K-12 |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli K-12 | |
| NADH | - |
Escherichia coli K-12 | |
| NADP+ | - |
Escherichia coli K-12 | |
| NADPH | - |
Escherichia coli K-12 | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli K-12 | isoelectric focusing | - |
6 |
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