BRENDA - Enzyme Database show
show all sequences of 1.1.1.94

Biosynthesis in Escherichia coli of sn-glycerol 3-phosphate, a precursor of phospholipid. Purification and physical characterization of wild type and feedback-resistant forms of the biosynthetic sn-glycerol-3-phosphate dehydrogenase

Edgar, J.R.; Bell, R.M.; J. Biol. Chem. 253, 6348-6353 (1978)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
existence of a naturally occurring feedback inhibition-resistant mutant in strain BB26-36-R2
Escherichia coli K-12
Inhibitors
Inhibitors
Commentary
Organism
Structure
sn-glycerol-3-phosphate
the wild-type enzyme is feedback inhibited
Escherichia coli K-12
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25900
-
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
26000
-
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
32500
-
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
48300
-
feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
49000
-
wild-type enzyme, gel filtration
Escherichia coli K-12
50000
-
feedback inhibition-resistant mutant, gel filtration
Escherichia coli K-12
50700
-
wild-type enzyme, sedimentation equilibrium analysis
Escherichia coli K-12
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerone phosphate + NAD(P)H
Escherichia coli K-12
the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview
sn-glycerol-3-phosphate + NAD(P)+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli K-12
-
and strain BB26-36-R2
-
Purification (Commentary)
Commentary
Organism
native wild-type enzyme from Escherichia coli K12 12000fold by streptomycin and ammonum sulfate fractionation, adsorption, hydroxy apatite, and anion exchange chromatography, and gel filtration to over 95% purity, feedback inhibition-resistant mutant from strain BB26-36-R2
Escherichia coli K-12
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
70
-
purified wild-type enzyme
Escherichia coli K-12
Storage Stability
Storage Stability
Organism
-20C, purified concentrated wild-type enzyme, 50% glycerol, 25 mM Tris-HCl, pH 7.4, 0.5 mM DTT, 0.2 M NaCl, 5 mM glycerol-3-phosphate, 1 month, over 90% remaining activity
Escherichia coli K-12
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + NAD(P)H
i.e. dihydroxyacetone phosphate
669174
Escherichia coli K-12
sn-glycerol-3-phosphate + NAD(P)+
-
-
-
r
glycerone phosphate + NAD(P)H
the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview
669174
Escherichia coli K-12
sn-glycerol-3-phosphate + NAD(P)+
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
23
-
assay at
Escherichia coli K-12
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli K-12
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli K-12
NADH
-
Escherichia coli K-12
NADP+
-
Escherichia coli K-12
NADPH
-
Escherichia coli K-12
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Escherichia coli K-12
isoelectric focusing
-
6
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli K-12
NADH
-
Escherichia coli K-12
NADP+
-
Escherichia coli K-12
NADPH
-
Escherichia coli K-12
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
existence of a naturally occurring feedback inhibition-resistant mutant in strain BB26-36-R2
Escherichia coli K-12
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
sn-glycerol-3-phosphate
the wild-type enzyme is feedback inhibited
Escherichia coli K-12
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25900
-
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
26000
-
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
32500
-
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
48300
-
feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
49000
-
wild-type enzyme, gel filtration
Escherichia coli K-12
50000
-
feedback inhibition-resistant mutant, gel filtration
Escherichia coli K-12
50700
-
wild-type enzyme, sedimentation equilibrium analysis
Escherichia coli K-12
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glycerone phosphate + NAD(P)H
Escherichia coli K-12
the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview
sn-glycerol-3-phosphate + NAD(P)+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
native wild-type enzyme from Escherichia coli K12 12000fold by streptomycin and ammonum sulfate fractionation, adsorption, hydroxy apatite, and anion exchange chromatography, and gel filtration to over 95% purity, feedback inhibition-resistant mutant from strain BB26-36-R2
Escherichia coli K-12
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
70
-
purified wild-type enzyme
Escherichia coli K-12
Storage Stability (protein specific)
Storage Stability
Organism
-20C, purified concentrated wild-type enzyme, 50% glycerol, 25 mM Tris-HCl, pH 7.4, 0.5 mM DTT, 0.2 M NaCl, 5 mM glycerol-3-phosphate, 1 month, over 90% remaining activity
Escherichia coli K-12
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glycerone phosphate + NAD(P)H
i.e. dihydroxyacetone phosphate
669174
Escherichia coli K-12
sn-glycerol-3-phosphate + NAD(P)+
-
-
-
r
glycerone phosphate + NAD(P)H
the enzyme is required for biosynthesis of sn-glycerol-3-phosphate, overview
669174
Escherichia coli K-12
sn-glycerol-3-phosphate + NAD(P)+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 32500, native wild-type enzyme, SDS-PAGE, 2 * 25900, wild-type enzyme, denaturing sedimentation equilibrium analysis, 2 * 26000, feedback inhibition-resistant mutant, sedimentation equilibrium analysis
Escherichia coli K-12
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
23
-
assay at
Escherichia coli K-12
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli K-12
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Escherichia coli K-12
isoelectric focusing
-
6
Other publictions for EC 1.1.1.94
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738799
Lakshmanan
In silico model-driven cofacto ...
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J. Ind. Microbiol. Biotechnol.
42
1401-1414
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1
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1
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1
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Rickettsia prowazekii uses an ...
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J. Bacteriol.
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1
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2
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1
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4
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1
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3
1
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2
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1
2
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1
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2
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1
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1
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3
1
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1
1
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712449
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Metabolic and transcriptional ...
Escherichia coli
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285
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2010
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1
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1
1
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-
689802
Yeh
Structure of glycerol-3-phosph ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
105
3280-3285
2008
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1
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3
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4
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4
1
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1
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3
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4
1
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690210
Watanabe
Expression of glycerol 3-phosp ...
Candida versatilis
Yeast
25
107-116
2008
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1
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3
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1
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1
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1
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656732
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Polyol accumulation by Aspergi ...
Aspergillus oryzae
Microbiology
150
1095-1101
2004
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1
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1
4
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1
4
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669177
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
255
3492-3497
1980
-
-
-
-
1
-
11
5
-
-
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
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1
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-
5
16
-
-
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-
-
5
-
1
-
-
11
16
5
-
-
-
1
-
-
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-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
669174
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli K-12
J. Biol. Chem.
253
6348-6353
1978
-
-
-
-
1
-
1
-
-
-
7
1
-
1
-
-
1
-
-
-
1
1
2
1
1
-
-
-
1
-
-
4
-
1
-
-
-
-
4
-
1
-
-
1
-
-
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-
7
1
-
-
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1
-
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1
1
2
1
1
-
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1
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-
1
-
-
-
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-
-
669175
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
253
6354-6363
1978
-
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1
-
4
1
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1
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2
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1
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2
-
1
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1
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4
3
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4
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1
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4
3
1
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1
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-
2
-
1
-
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-
1
-
-
-
-
-
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-
-
-
287523
Kito
Purification and regulatory pr ...
Escherichia coli
J. Biol. Chem.
244
3316-3323
1969
-
-
-
-
-
-
11
3
-
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-
1
-
1
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1
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1
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1
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1
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3
-
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3
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11
-
3
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1
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1
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1
-
1
-
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1
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287524
Kim
Properties of the nicotinamide ...
Oryctolagus cuniculus
J. Biol. Chem.
243
3351-3356
1968
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16
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1
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1
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1
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16
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