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show all sequences of 1.1.1.94

Purification and regulatory properties of the biosynthetic L-glycerol 3-phosphate dehydrogenase from Escherichia coli

Kito, M.; Pizer, L.I.; J. Biol. Chem. 244, 3316-3323 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
100 mM, 68% inhibition, 10 mM, 8% inhibition
Escherichia coli
DL-glycerol 3-phosphate
0.05 mM, 26% inhibition
Escherichia coli
glycerol 3-phosphate
50% inhibition with 0.035 mM, sigmoid inhibition curve, competitive inhibition vs. glycerone phosphate, uncompetitive vs. NADPH
Escherichia coli
K2PO4-
100 mM, 95% inhibition, 10 mM, 27% inhibition
Escherichia coli
K2SO4
100 mM, 60% inhibition
Escherichia coli
KCl
100 mM, 47% inhibition
Escherichia coli
Na2PO4-
100 mM, 92% inhibition, 10 mM, 27% inhibition
Escherichia coli
Na2SO4
100 mM, 63% inhibition
Escherichia coli
NaCl
100 mM, 47% inhibition
Escherichia coli
NH4Cl
100 mM, 37% inhibition
Escherichia coli
palmitoyl-CoA
0.0015 mM, 52% inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
-
Escherichia coli
0.17
-
glycerone phosphate
-
Escherichia coli
0.21
-
glycerol-3-phosphate
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sn-glycerol 3-phosphate + NAD(P)+
Escherichia coli
-
glycerone phosphate + NAD(P)H + H+
-
Escherichia coli
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-150, DEAE-Sephadex
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4800
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol 3-phosphate + NAD(P)+
-
287523
Escherichia coli
glycerone phosphate + NAD(P)H + H+
-
287523
Escherichia coli
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
deamino-NAD+
2fold activity compared to NAD+
Escherichia coli
NAD+
-
Escherichia coli
NADP+
2fold activity compared to NAD+
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
deamino-NAD+
2fold activity compared to NAD+
Escherichia coli
NAD+
-
Escherichia coli
NADP+
2fold activity compared to NAD+
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(NH4)2SO4
100 mM, 68% inhibition, 10 mM, 8% inhibition
Escherichia coli
DL-glycerol 3-phosphate
0.05 mM, 26% inhibition
Escherichia coli
glycerol 3-phosphate
50% inhibition with 0.035 mM, sigmoid inhibition curve, competitive inhibition vs. glycerone phosphate, uncompetitive vs. NADPH
Escherichia coli
K2PO4-
100 mM, 95% inhibition, 10 mM, 27% inhibition
Escherichia coli
K2SO4
100 mM, 60% inhibition
Escherichia coli
KCl
100 mM, 47% inhibition
Escherichia coli
Na2PO4-
100 mM, 92% inhibition, 10 mM, 27% inhibition
Escherichia coli
Na2SO4
100 mM, 63% inhibition
Escherichia coli
NaCl
100 mM, 47% inhibition
Escherichia coli
NH4Cl
100 mM, 37% inhibition
Escherichia coli
palmitoyl-CoA
0.0015 mM, 52% inhibition
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADPH
-
Escherichia coli
0.17
-
glycerone phosphate
-
Escherichia coli
0.21
-
glycerol-3-phosphate
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
sn-glycerol 3-phosphate + NAD(P)+
Escherichia coli
-
glycerone phosphate + NAD(P)H + H+
-
Escherichia coli
r
Purification (Commentary) (protein specific)
Commentary
Organism
streptomycin, ammonium sulfate, DEAE-Sephadex, Sephadex G-150, DEAE-Sephadex
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4800
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
sn-glycerol 3-phosphate + NAD(P)+
-
287523
Escherichia coli
glycerone phosphate + NAD(P)H + H+
-
287523
Escherichia coli
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
-
Escherichia coli
Other publictions for EC 1.1.1.94
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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689802
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669177
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Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
255
3492-3497
1980
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1
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11
5
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1
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2
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5
16
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5
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669174
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J. Biol. Chem.
253
6348-6353
1978
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1
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1
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7
1
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1
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1
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1
1
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669175
Edgar
Biosynthesis in Escherichia co ...
Escherichia coli
J. Biol. Chem.
253
6354-6363
1978
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1
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4
1
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1
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3
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3
1
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1
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287523
Kito
Purification and regulatory pr ...
Escherichia coli
J. Biol. Chem.
244
3316-3323
1969
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-
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11
3
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1
-
1
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1
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1
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1
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1
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3
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3
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11
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3
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1
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1
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1
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1
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287524
Kim
Properties of the nicotinamide ...
Oryctolagus cuniculus
J. Biol. Chem.
243
3351-3356
1968
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16
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1
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16
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