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Literature summary for 1.1.1.93 extracted from

  • Malik, R.; Viola, R.E.
    Structural characterization of tartrate dehydrogenase: a versatile enzyme catalyzing multiple reactions (2010), Acta Crystallogr. Sect. D, 66, 673-684.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with the intermediate analog oxalate, Mg2+ and NADH, to 2.0 A resolution Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
D225A mutation in metal ion-binding ligand, 20fold decrease in metal ion-binding affinity and a two-orders-of-magnitude decrease in catalysis Pseudomonas putida
D250A mutation in metal ion-binding ligand, 10fold decrease in metal ion-binding affinity and a two-orders-of-magnitude decrease in catalysis Pseudomonas putida
R108L 1.3% of wild-type catalytic rate Pseudomonas putida
R108Q 8.7% of wild-type catalytic rate Pseudomonas putida
R98L mutant is not expressed in stable form Pseudomonas putida
R98Q 2.6% of wild-type catalytic rate Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NAD+ wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.079
-
D-malate wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.093
-
D-malate mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.096
-
NAD+ mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.098
-
D-malate mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.135
-
NAD+ mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.18
-
D-malate mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.24
-
NAD+ mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pseudomonas putida

Reaction

Reaction Comment Organism Reaction ID
tartrate + NAD+ = oxaloglycolate + NADH + H+ lysyl amino group, Lys192 is the base responsible for the water-mediated proton abstraction from the C2 hydroxyl group of the substrate that begins the catalytic reaction, followed by hydride transfer to NAD. The hydroxyl group of Tyr141 functions as a general acid to protonate the enolate intermediate. Each substrate undergoes the initial hydride transfer Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-malate + NAD+
-
Pseudomonas putida pyruvate + CO2 + NADH + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
NAD+ mutant D225A, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.06
-
NAD+ mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.087
-
NAD+ mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.088
-
NAD+ mutant D250A, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.175
-
NAD+ mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
6.5
-
NAD+ wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.36
-
NAD+ mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
0.48
-
D-malate mutant R108L, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.8
-
NAD+ mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
1.8
-
D-malate mutant R98Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
4.3
-
NAD+ mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
6.2
-
D-malate mutant R108Q, pH 7.5, temperature not specified in the publication Pseudomonas putida
82
-
D-malate wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida
260
-
NAD+ wild-type, pH 7.5, temperature not specified in the publication Pseudomonas putida