BRENDA - Enzyme Database show
show all sequences of 1.1.1.93

Tartaric acid metabolism. V. Crystalline tartrate dehydrogenase

Kohn, L.D.; Packman, P.M.; Allen, R.H.; Jakoby, W.B.; J. Biol. Chem. 243, 2479-2485 (1968)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NAD+
-
Pseudomonas putida
0.83
-
meso-tartrate
-
Pseudomonas putida
1.4
-
NADH
in presence of 1.5 mM dihydroxyfumarate
Pseudomonas putida
11
-
Dihydroxyfumarate
in presence of 0.19 mM NADH
Pseudomonas putida
55
-
L-Tartrate
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36800
-
4 * 36800, sedimentation study after dialysis with guanidine-mercaptoethanol solution
Pseudomonas putida
145000
-
diffusion and sedimentation data
Pseudomonas putida
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.71
-
-
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tartrate + NAD+
-
389610
Pseudomonas putida
oxaloglycolate + NADH + H+
-
389610
Pseudomonas putida
r
meso-tartrate + NAD+
-
389610
Pseudomonas putida
oxaloglycolate + NADH + H+
-
389610
Pseudomonas putida
r
oxaloglycolate + NADH
-
389610
Pseudomonas putida
tartrate + NAD+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 36800, sedimentation study after dialysis with guanidine-mercaptoethanol solution
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
dihydroxyfumarate + NADH
Pseudomonas putida
8.5
-
meso-tartrate + NAD+
Pseudomonas putida
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.8
pH 6: about 40% of activity maximum, pH 7.8: about 80% of activity maximum, dihydroxyfumarate + NADH
Pseudomonas putida
7
9
at pH 7 and pH 9: about 50% of activity maximum, mesotartrate + NAD+
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
cofactor
Pseudomonas putida
NADH
cofactor
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
cofactor
Pseudomonas putida
NADH
cofactor
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.09
-
NAD+
-
Pseudomonas putida
0.83
-
meso-tartrate
-
Pseudomonas putida
1.4
-
NADH
in presence of 1.5 mM dihydroxyfumarate
Pseudomonas putida
11
-
Dihydroxyfumarate
in presence of 0.19 mM NADH
Pseudomonas putida
55
-
L-Tartrate
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
required
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36800
-
4 * 36800, sedimentation study after dialysis with guanidine-mercaptoethanol solution
Pseudomonas putida
145000
-
diffusion and sedimentation data
Pseudomonas putida
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.71
-
-
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tartrate + NAD+
-
389610
Pseudomonas putida
oxaloglycolate + NADH + H+
-
389610
Pseudomonas putida
r
meso-tartrate + NAD+
-
389610
Pseudomonas putida
oxaloglycolate + NADH + H+
-
389610
Pseudomonas putida
r
oxaloglycolate + NADH
-
389610
Pseudomonas putida
tartrate + NAD+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 36800, sedimentation study after dialysis with guanidine-mercaptoethanol solution
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
dihydroxyfumarate + NADH
Pseudomonas putida
8.5
-
meso-tartrate + NAD+
Pseudomonas putida
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.8
pH 6: about 40% of activity maximum, pH 7.8: about 80% of activity maximum, dihydroxyfumarate + NADH
Pseudomonas putida
7
9
at pH 7 and pH 9: about 50% of activity maximum, mesotartrate + NAD+
Pseudomonas putida
Other publictions for EC 1.1.1.93
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
710720
Malik
Structural characterization of ...
Pseudomonas putida
Acta Crystallogr. Sect. D
66
673-684
2010
-
-
1
1
6
-
-
8
-
-
-
-
-
2
-
-
1
1
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
1
6
-
-
-
-
8
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
8
8
667741
Karsten
An isothermal titration calori ...
Pseudomonas putida
Biochemistry
45
9000-9006
2006
-
-
-
-
-
-
-
1
-
2
-
-
-
2
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654622
Karsten
Tartrate dehydrogenase catalyz ...
Escherichia coli
Biochemistry
41
12193-12199
2002
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
389612
Tipton
-
Tartrate dehydrogenase, an enz ...
Pseudomonas putida
Protein Pept. Lett.
7
323-332
2000
1
-
-
-
-
-
3
4
-
4
-
1
-
1
-
-
-
1
-
-
-
-
5
-
-
-
-
4
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
4
-
4
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
389619
Tsukatani
-
Quantification of L-tartrate i ...
Escherichia coli
Anal. Sci.
16
265-268
2000
-
1
-
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389613
Harve
Production and purification of ...
Pseudomonas putida
Appl. Biochem. Biotechnol.
70-72
677-686
1998
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389614
Tipton
Transient-state kinetic analys ...
Pseudomonas putida
Biochemistry
35
3108-3114
1996
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
349359
Serfozo
Substrate determinants of the ...
Pseudomonas putida
Biochemistry
34
7517-7524
1995
-
-
-
-
-
-
-
14
-
-
-
-
-
1
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349358
Beecher
Tartrate dehydrogenase-oxalate ...
Pseudomonas putida
Arch. Biochem. Biophys.
315
255-261
1994
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389617
Tipton
Tartrate dehydrogenase, a new ...
Pseudomonas putida
Arch. Biochem. Biophys.
313
15-21
1994
-
-
1
-
-
-
-
3
-
-
1
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
389618
Tipton
Characterization of the multip ...
Pseudomonas putida
Biochemistry
29
1749-1756
1990
-
-
-
-
-
-
1
-
-
2
2
-
-
2
-
-
1
1
-
-
1
1
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
2
2
-
-
-
-
1
-
-
1
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389611
Gifforn
-
L-(+)-Tartrate ...
Rhodobacter sphaeroides
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
7
78-85
1985
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
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-
-
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287429
Ebbighausen
-
A novel mechanism involved in ...
Rhodobacter sphaeroides
Arch. Microbiol.
138
338-344
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389609
Giffhorn
Purification and characterizat ...
Rhodobacter sphaeroides
J. Bacteriol.
155
281-290
1983
1
-
-
-
-
1
4
4
-
6
3
-
-
2
-
-
1
-
-
-
1
1
2
1
1
-
1
-
1
1
-
1
-
-
-
1
-
-
1
-
-
1
-
4
-
4
-
6
3
-
-
-
-
1
-
-
1
1
2
1
1
-
1
-
1
1
-
-
-
-
-
-
-
-
389610
Kohn
Tartaric acid metabolism. V. C ...
Pseudomonas putida
J. Biol. Chem.
243
2479-2485
1968
-
-
-
1
-
-
-
5
-
1
2
-
-
1
-
-
1
-
-
-
1
-
3
1
-
-
-
-
2
2
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
5
-
1
2
-
-
-
-
1
-
-
1
-
3
1
-
-
-
-
2
2
-
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-
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-
-
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