Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.92 extracted from

  • Kohn, L.D.; Jakoby W., B.
    Tartaric acid metabolism. VI. Crystalline oxaloglcolate reductive decarboxylase (1968), J. Biol. Chem., 243, 2486-2493.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
NADPH 2 mM 2,3-dihydroxysuccinate as substrate Pseudomonas putida
0.024
-
NADPH 2 mM hydroxypyruvate as substrate Pseudomonas putida
0.14
-
NADH 2 mM 2,3-dihydroxysuccinate as substrate Pseudomonas putida
0.17
-
NADH 2 mM hydroxypyruvate as substrate Pseudomonas putida
1
-
Hydroxypyruvate cofactor NADH Pseudomonas putida
1.2
-
2,3-dihydroxysuccinate cofactor NADH Pseudomonas putida
2.5
-
Hydroxypyruvate cofactor NADPH Pseudomonas putida
2.5
-
2,3-dihydroxysuccinate cofactor NADPH Pseudomonas putida
3.3
-
glyoxylate cofactor NADH Pseudomonas putida
8
-
glyoxylate cofactor NADPH Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
sedimentation equilibrium Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxy-3-oxosuccinate + NAD(P)H Pseudomonas putida
-
D-glycerate + NAD(P)+ + CO2
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-100, disc electrophoresis, Sephadex G-100, crystallization Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
substrate 2,3-dihydroxy-succinate Pseudomonas putida
7.7
-
substrate hydroxypyruvate Pseudomonas putida

Storage Stability

Storage Stability Organism
-15°C, 40 mM Tris-HCl, 30% glycerol, 5 mM mercaptoethanol, 6 months, 10% loss of activity Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxysuccinate + NAD(P)H
-
Pseudomonas putida D-glycerate + NAD(P)+ + CO2
-
ir
2-hydroxy-3-oxosuccinate + NAD(P)H
-
Pseudomonas putida D-glycerate + NAD(P)+ + CO2
-
r
2-hydroxy-3-oxosuccinate + NAD(P)H enzyme is inhibited at higher levels of ionic strength Pseudomonas putida D-glycerate + NAD(P)+ + CO2
-
r
2-hydroxy-3-oxosuccinate + NADH + H+
-
Pseudomonas putida D-glycerate + NAD+ + CO2
-
r
glyoxylate + NAD(P)H
-
Pseudomonas putida glycolic acid + NAD(P)+
-
ir
hydroxypyruvate + NAD(P)H
-
Pseudomonas putida D-glycerate + NAD(P)+ + H+
-
ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Pseudomonas putida