BRENDA - Enzyme Database show
show all sequences of 1.1.1.92

Tartaric acid metabolism. VI. Crystalline oxaloglcolate reductive decarboxylase

Kohn, L.D.; Jakoby W., B.; J. Biol. Chem. 243, 2486-2493 (1968)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADPH
2 mM 2,3-dihydroxysuccinate as substrate
Pseudomonas putida
0.024
-
NADPH
2 mM hydroxypyruvate as substrate
Pseudomonas putida
0.14
-
NADH
2 mM 2,3-dihydroxysuccinate as substrate
Pseudomonas putida
0.17
-
NADH
2 mM hydroxypyruvate as substrate
Pseudomonas putida
1
-
Hydroxypyruvate
cofactor NADH
Pseudomonas putida
1.2
-
2,3-dihydroxysuccinate
cofactor NADH
Pseudomonas putida
2.5
-
2,3-dihydroxysuccinate
cofactor NADPH
Pseudomonas putida
2.5
-
Hydroxypyruvate
cofactor NADPH
Pseudomonas putida
3.3
-
glyoxylate
cofactor NADH
Pseudomonas putida
8
-
glyoxylate
cofactor NADPH
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
sedimentation equilibrium
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-hydroxy-3-oxosuccinate + NAD(P)H
Pseudomonas putida
-
D-glycerate + NAD(P)+ + CO2
-
Pseudomonas putida
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-100, disc electrophoresis, Sephadex G-100, crystallization
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6
-
substrate 2,3-dihydroxy-succinate
Pseudomonas putida
7.7
-
substrate hydroxypyruvate
Pseudomonas putida
Storage Stability
Storage Stability
Organism
-15░C, 40 mM Tris-HCl, 30% glycerol, 5 mM mercaptoethanol, 6 months, 10% loss of activity
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxysuccinate + NAD(P)H
-
287510
Pseudomonas putida
D-glycerate + NAD(P)+ + CO2
-
287510
Pseudomonas putida
ir
2-hydroxy-3-oxosuccinate + NAD(P)H
-
287510
Pseudomonas putida
D-glycerate + NAD(P)+ + CO2
-
287510
Pseudomonas putida
r
2-hydroxy-3-oxosuccinate + NAD(P)H
enzyme is inhibited at higher levels of ionic strength
287510
Pseudomonas putida
D-glycerate + NAD(P)+ + CO2
-
287510
Pseudomonas putida
r
glyoxylate + NAD(P)H
-
287510
Pseudomonas putida
glycolic acid + NAD(P)+
-
287510
Pseudomonas putida
ir
hydroxypyruvate + NAD(P)H
-
287510
Pseudomonas putida
D-glycerate + NAD(P)+
-
287510
Pseudomonas putida
ir
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
NADPH
2 mM 2,3-dihydroxysuccinate as substrate
Pseudomonas putida
0.024
-
NADPH
2 mM hydroxypyruvate as substrate
Pseudomonas putida
0.14
-
NADH
2 mM 2,3-dihydroxysuccinate as substrate
Pseudomonas putida
0.17
-
NADH
2 mM hydroxypyruvate as substrate
Pseudomonas putida
1
-
Hydroxypyruvate
cofactor NADH
Pseudomonas putida
1.2
-
2,3-dihydroxysuccinate
cofactor NADH
Pseudomonas putida
2.5
-
2,3-dihydroxysuccinate
cofactor NADPH
Pseudomonas putida
2.5
-
Hydroxypyruvate
cofactor NADPH
Pseudomonas putida
3.3
-
glyoxylate
cofactor NADH
Pseudomonas putida
8
-
glyoxylate
cofactor NADPH
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63000
-
sedimentation equilibrium
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-hydroxy-3-oxosuccinate + NAD(P)H
Pseudomonas putida
-
D-glycerate + NAD(P)+ + CO2
-
Pseudomonas putida
r
Purification (Commentary) (protein specific)
Commentary
Organism
protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-100, disc electrophoresis, Sephadex G-100, crystallization
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.6
-
substrate 2,3-dihydroxy-succinate
Pseudomonas putida
7.7
-
substrate hydroxypyruvate
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
-15░C, 40 mM Tris-HCl, 30% glycerol, 5 mM mercaptoethanol, 6 months, 10% loss of activity
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxysuccinate + NAD(P)H
-
287510
Pseudomonas putida
D-glycerate + NAD(P)+ + CO2
-
287510
Pseudomonas putida
ir
2-hydroxy-3-oxosuccinate + NAD(P)H
-
287510
Pseudomonas putida
D-glycerate + NAD(P)+ + CO2
-
287510
Pseudomonas putida
r
2-hydroxy-3-oxosuccinate + NAD(P)H
enzyme is inhibited at higher levels of ionic strength
287510
Pseudomonas putida
D-glycerate + NAD(P)+ + CO2
-
287510
Pseudomonas putida
r
glyoxylate + NAD(P)H
-
287510
Pseudomonas putida
glycolic acid + NAD(P)+
-
287510
Pseudomonas putida
ir
hydroxypyruvate + NAD(P)H
-
287510
Pseudomonas putida
D-glycerate + NAD(P)+
-
287510
Pseudomonas putida
ir
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Pseudomonas putida
Other publictions for EC 1.1.1.92
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287509
Do Nascimento
The stereospecificity of seque ...
Pseudomonas putida
Biochem. J.
149
553-557
1975
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-
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-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287510
Kohn
Tartaric acid metabolism. VI. ...
Pseudomonas putida
J. Biol. Chem.
243
2486-2493
1968
-
-
-
-
-
-
-
10
-
-
1
1
-
1
-
-
1
-
-
-
2
1
5
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
10
-
-
1
1
-
-
-
1
-
-
2
1
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-