BRENDA - Enzyme Database show
show all sequences of 1.1.1.91

Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767

Yang, D.D.; Francois, J.M.; de Billerbeck, G.M.; BMC Microbiol. 12, 126 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as a His-tagged fusion protein
Phanerochaete chrysosporium
Inhibitors
Inhibitors
Commentary
Organism
Structure
3,4-dimethoxybenzaldehyde
-
Phanerochaete chrysosporium
3,4-dimethoxybenzyl alcohol
-
Phanerochaete chrysosporium
3,5-dimethoxybenzaldehyde
-
Phanerochaete chrysosporium
3-hydroxy-4-methoxy-benzaldehyde
-
Phanerochaete chrysosporium
4-hydroxy-3-methoxybenzaldehyde
-
Phanerochaete chrysosporium
5-(hydroxymethyl)-2-furaldehyde
-
Phanerochaete chrysosporium
benzaldehyde
-
Phanerochaete chrysosporium
trans-cinnamaldehyde
-
Phanerochaete chrysosporium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.022
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.038
-
NADP+
pH 6.1, 30°C
Phanerochaete chrysosporium
0.039
-
NADPH
pH 6.1, 30°C
Phanerochaete chrysosporium
0.09
-
4-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.22
-
NADH
pH 6.1, 30°C
Phanerochaete chrysosporium
0.27
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.37
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
0.53
-
phenylacetaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
1.4
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
1.4
-
4-Hydroxy-3-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
1.7
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.4
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
25
-
4-hydroxy-3-methoxy-benzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE
Phanerochaete chrysosporium
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phanerochaete chrysosporium
-
-
-
Purification (Commentary)
Commentary
Organism
using Ni-NTA chromatography
Phanerochaete chrysosporium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-fluorobenzaldehyde + NADPH
relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
2-fluorobenzyl alcohol + NADP+
-
-
-
r
2-methoxybenzaldehyde + NADPH
relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
2-methoxybenzyl alcohol + NADP+
-
-
-
r
2-nitrobenzaldehyde + NADPH
relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
2-nitrobenzyl alcohol + NADP+
-
-
-
r
3,4-dimethoxybenzaldehyde + NADH
-
724669
Phanerochaete chrysosporium
3,4-dimethoxybenzyl alcohol + NAD+
-
-
-
r
3,4-dimethoxybenzaldehyde + NADPH
relative activity: 100% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3,4-dimethoxybenzyl alcohol + NADP+
-
-
-
r
3,5-dimethoxybenzaldehyde + NADPH
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3,5-dimethoxybenzyl alcohol + NADP+
-
-
-
r
3-chlorobenzaldehyde + NADPH
relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-chlorobenzyl alcohol + NADP+
-
-
-
r
3-fluorobenzaldehyde + NADPH
relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-fluorobenzyl alcohol + NADP+
-
-
-
r
3-hydroxy-4-methoxy-benzaldehyde + NADPH
relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-hydroxy-4-methoxy-benzyl alcohol + NADP+
-
-
-
r
3-methoxybenzaldehyde + NADPH
relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-methoxybenzyl alcohol + NADP+
-
-
-
r
3-nitrobenzaldehyde + NADPH
relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-nitrobenzyl alcohol + NADP+
-
-
-
r
4-chlorobenzaldehyde + NADPH
relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-chlorobenzyl alcohol + NADP+
-
-
-
r
4-fluorobenzaldehyde + NADPH
relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-fluorobenzyl alcohol + NADP+
-
-
-
r
4-hydroxy-3-methoxy-benzaldehyde + NADPH
relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-hydroxy-3-methoxy-benzyl alcohol + NADP+
-
-
-
r
4-hydroxybenzaldehyde + NADPH
relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-hydroxybenzyl alcohol + NADP+
-
-
-
r
4-methoxybenzaldehyde + NADPH
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-methoxybenzyl alcohol + NADP+
-
-
-
r
4-nitrobenzaldehyde + NADPH
relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-nitrobenzyl alcohol + NADP+
-
-
-
r
5-(hydroxymethyl)-2-furaldehyde + NADPH + H+
relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
r
benzaldehyde + NADPH
relative activity: 42% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
benzyl alcohol + NADP+
-
-
-
r
decanal + NADPH
relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
decanol + NADP+
-
-
-
r
heptaldehyde + NADPH
relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
?
-
-
-
r
hexanal + NADPH
relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
hexanol + NADP+
-
-
-
r
nonanal + NADPH
relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
nonanol + NADP+
-
-
-
r
octanal + NADPH
relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
octanol + NADP+
-
-
-
r
pentanal + NADPH
relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
pentanol + NADP+
-
-
-
r
phenylacetaldehyde + NADPH
relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
phenylethanol + NADP+
-
-
-
r
trans-cinnamaldehyde + NADPH
relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
?
-
-
-
r
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Phanerochaete chrysosporium
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
NADH
pH 6.1, 30°C
Phanerochaete chrysosporium
2.55
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
2.56
-
NADP+
pH 6.1, 30°C
Phanerochaete chrysosporium
2.93
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.33
-
4-Hydroxy-3-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.83
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
4.33
-
4-hydroxy-3-methoxy-benzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
7.16
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
8.16
-
4-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
8.833
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
9.83
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
11.16
-
phenylacetaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
11.16
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
11.33
-
NADPH
pH 6.1, 30°C
Phanerochaete chrysosporium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
reduction activity
Phanerochaete chrysosporium
10.4
-
oxidation activity
Phanerochaete chrysosporium
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
2.3
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.4
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.5
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
5.1
-
4-hydroxy-3-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
81
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
136
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
165
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as a His-tagged fusion protein
Phanerochaete chrysosporium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,4-dimethoxybenzaldehyde
-
Phanerochaete chrysosporium
3,4-dimethoxybenzyl alcohol
-
Phanerochaete chrysosporium
3,5-dimethoxybenzaldehyde
-
Phanerochaete chrysosporium
3-hydroxy-4-methoxy-benzaldehyde
-
Phanerochaete chrysosporium
4-hydroxy-3-methoxybenzaldehyde
-
Phanerochaete chrysosporium
5-(hydroxymethyl)-2-furaldehyde
-
Phanerochaete chrysosporium
benzaldehyde
-
Phanerochaete chrysosporium
trans-cinnamaldehyde
-
Phanerochaete chrysosporium
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.1
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
2.3
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.4
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.5
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
5.1
-
4-hydroxy-3-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
81
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
136
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
165
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.022
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.038
-
NADP+
pH 6.1, 30°C
Phanerochaete chrysosporium
0.039
-
NADPH
pH 6.1, 30°C
Phanerochaete chrysosporium
0.09
-
4-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.22
-
NADH
pH 6.1, 30°C
Phanerochaete chrysosporium
0.27
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
0.37
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
0.53
-
phenylacetaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
1.4
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
1.4
-
4-Hydroxy-3-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
1.7
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.4
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
25
-
4-hydroxy-3-methoxy-benzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE
Phanerochaete chrysosporium
Purification (Commentary) (protein specific)
Commentary
Organism
using Ni-NTA chromatography
Phanerochaete chrysosporium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-fluorobenzaldehyde + NADPH
relative activity: 27% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
2-fluorobenzyl alcohol + NADP+
-
-
-
r
2-methoxybenzaldehyde + NADPH
relative activity: 58% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
2-methoxybenzyl alcohol + NADP+
-
-
-
r
2-nitrobenzaldehyde + NADPH
relative activity: 57% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
2-nitrobenzyl alcohol + NADP+
-
-
-
r
3,4-dimethoxybenzaldehyde + NADH
-
724669
Phanerochaete chrysosporium
3,4-dimethoxybenzyl alcohol + NAD+
-
-
-
r
3,4-dimethoxybenzaldehyde + NADPH
relative activity: 100% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3,4-dimethoxybenzyl alcohol + NADP+
-
-
-
r
3,5-dimethoxybenzaldehyde + NADPH
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3,5-dimethoxybenzyl alcohol + NADP+
-
-
-
r
3-chlorobenzaldehyde + NADPH
relative activity: 73% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-chlorobenzyl alcohol + NADP+
-
-
-
r
3-fluorobenzaldehyde + NADPH
relative activity: 69% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-fluorobenzyl alcohol + NADP+
-
-
-
r
3-hydroxy-4-methoxy-benzaldehyde + NADPH
relative activity: 17% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-hydroxy-4-methoxy-benzyl alcohol + NADP+
-
-
-
r
3-methoxybenzaldehyde + NADPH
relative activity: 117% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-methoxybenzyl alcohol + NADP+
-
-
-
r
3-nitrobenzaldehyde + NADPH
relative activity: 25% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
3-nitrobenzyl alcohol + NADP+
-
-
-
r
4-chlorobenzaldehyde + NADPH
relative activity: 61% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-chlorobenzyl alcohol + NADP+
-
-
-
r
4-fluorobenzaldehyde + NADPH
relative activity: 29% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-fluorobenzyl alcohol + NADP+
-
-
-
r
4-hydroxy-3-methoxy-benzaldehyde + NADPH
relative activity: 19% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-hydroxy-3-methoxy-benzyl alcohol + NADP+
-
-
-
r
4-hydroxybenzaldehyde + NADPH
relative activity: 34% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-hydroxybenzyl alcohol + NADP+
-
-
-
r
4-methoxybenzaldehyde + NADPH
relative activity: 110% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-methoxybenzyl alcohol + NADP+
-
-
-
r
4-nitrobenzaldehyde + NADPH
relative activity: 31% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
4-nitrobenzyl alcohol + NADP+
-
-
-
r
5-(hydroxymethyl)-2-furaldehyde + NADPH + H+
relative activity: 36% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
(furan-2,5-diyl)dimethanol + NADP+
-
-
-
r
benzaldehyde + NADPH
relative activity: 42% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
benzyl alcohol + NADP+
-
-
-
r
decanal + NADPH
relative activity: 2% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
decanol + NADP+
-
-
-
r
heptaldehyde + NADPH
relative activity: 26% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
?
-
-
-
r
hexanal + NADPH
relative activity: 47% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
hexanol + NADP+
-
-
-
r
nonanal + NADPH
relative activity: 3% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
nonanol + NADP+
-
-
-
r
octanal + NADPH
relative activity: 37% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
octanol + NADP+
-
-
-
r
pentanal + NADPH
relative activity: 8% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
pentanol + NADP+
-
-
-
r
phenylacetaldehyde + NADPH
relative activity: 109 (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
phenylethanol + NADP+
-
-
-
r
trans-cinnamaldehyde + NADPH
relative activity: 39% (reference substrate: 3,4-dimethoxybenzaldehyde)
724669
Phanerochaete chrysosporium
?
-
-
-
r
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Phanerochaete chrysosporium
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2
-
NADH
pH 6.1, 30°C
Phanerochaete chrysosporium
2.55
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
2.56
-
NADP+
pH 6.1, 30°C
Phanerochaete chrysosporium
2.93
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.33
-
4-Hydroxy-3-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.83
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
4.33
-
4-hydroxy-3-methoxy-benzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
7.16
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
8.16
-
4-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
8.833
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
9.83
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
11.16
-
phenylacetaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
11.16
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
11.33
-
NADPH
pH 6.1, 30°C
Phanerochaete chrysosporium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
reduction activity
Phanerochaete chrysosporium
10.4
-
oxidation activity
Phanerochaete chrysosporium
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.166
-
4-hydroxy-3-methoxy-benzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
2.33
-
4-Hydroxy-3-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
2.66
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.33
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
5
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
6.8
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
10
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
10
-
NADH
pH 6.1, 30°C
Phanerochaete chrysosporium
11.16
-
phenylacetaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
68
-
NADP+
pH 6.1, 30°C
Phanerochaete chrysosporium
90
-
4-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
283
-
NADPH
pH 6.1, 30°C
Phanerochaete chrysosporium
450
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
733
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.166
-
4-hydroxy-3-methoxy-benzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
2.33
-
4-Hydroxy-3-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
2.66
-
3-hydroxy-4-methoxy-benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
3.33
-
trans-cinnamaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
5
-
benzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
6.8
-
3,4-dimethoxybenzyl alcohol
pH 6.1, 30°C
Phanerochaete chrysosporium
10
-
5-(hydroxymethyl)-2-furaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
10
-
NADH
pH 6.1, 30°C
Phanerochaete chrysosporium
11.16
-
phenylacetaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
68
-
NADP+
pH 6.1, 30°C
Phanerochaete chrysosporium
90
-
4-methoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
283
-
NADPH
pH 6.1, 30°C
Phanerochaete chrysosporium
450
-
3,5-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
733
-
3,4-dimethoxybenzaldehyde
pH 6.1, 30°C
Phanerochaete chrysosporium
Other publictions for EC 1.1.1.91
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738738
Magomedova
Characterization of two novel ...
Cupriavidus necator, Cupriavidus necator H16
J. Biotechnol.
221
78-90
2016
-
-
1
-
-
-
-
2
-
-
-
-
-
38
-
-
1
-
-
-
-
-
15
-
2
-
-
2
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
15
-
2
-
-
2
2
-
-
-
-
-
-
-
2
2
739695
Kovi
Global transcriptome changes i ...
Lolium perenne
Sci. Rep.
6
28702
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735498
Ying
Characterization of an allylic ...
Yokenella sp. WZY002
Appl. Environ. Microbiol.
80
2399-2409
2014
3
-
1
-
-
-
7
6
-
3
1
-
4
1
-
-
1
-
-
-
-
-
13
1
2
-
1
6
2
-
-
2
-
-
-
3
-
1
2
-
-
-
-
7
-
6
-
3
1
-
4
-
-
1
-
-
-
-
13
1
2
-
1
6
2
-
-
-
-
-
-
-
6
6
737956
Ken
-
Biochemical characterization o ...
Taiwanofungus camphoratus
Bot. Stud.
55
14
2014
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
1
-
-
8
1
-
-
1
-
1
1
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
8
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
737897
Lerchner
Crystallographic analysis and ...
Ralstonia sp., Ralstonia sp. DSMZ 6428
Biotechnol. Bioeng.
110
2803-2814
2013
-
-
-
1
3
-
-
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
724669
Yang
Cloning, expression and charac ...
Phanerochaete chrysosporium
BMC Microbiol.
12
126
2012
-
-
1
-
-
-
8
14
-
-
1
-
-
1
-
-
1
-
-
-
-
-
27
-
1
-
-
14
2
-
-
-
8
-
-
-
-
1
-
-
-
-
-
8
8
14
-
-
1
-
-
-
-
1
-
-
-
-
27
-
1
-
-
14
2
-
-
-
-
-
-
-
14
14
389607
Gutierrez
Anisaldehyde production and ar ...
Pleurotus pulmonarius
Appl. Environ. Microbiol.
60
1783-1788
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389606
Muheim
Purification and properties of ...
Phanerochaete chrysosporium
Eur. J. Biochem.
195
369-375
1991
-
-
-
-
-
-
5
-
-
-
2
-
-
1
-
-
1
-
-
-
-
2
11
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
2
-
-
-
-
1
-
-
-
2
11
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288179
Kato
-
Reductive transformation of be ...
Nocardia asteroides, Nocardia asteroides JCM3016
J. Ferment. Bioeng.
69
220-223
1990
-
-
-
-
-
-
-
-
-
1
-
3
-
2
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
389479
Dalkin
Stress responses in alfalfa (M ...
Medicago sativa
Plant Physiol.
92
440-446
1990
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
-
-
-
-
-
-
16
-
-
-
-
-
-
8
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389602
Rhodes
-
Changes in the activity of enz ...
Solanum lycopersicum
Phytochemistry
16
655-659
1977
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389603
Mansell
-
Multiple forms and specificity ...
Picea abies
Phytochemistry
15
1849-1853
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389601
Rhodes
-
Enzymes involved in the reduct ...
Brassica napus subsp. rapifera
Phytochemistry
14
1235-1240
1975
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389598
Davies
-
Aromatic alcohol dehydrogenase ...
Solanum tuberosum
Phytochemistry
12
531-536
1973
-
-
-
-
-
-
-
21
-
-
-
-
-
1
-
-
1
-
-
1
-
1
15
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
21
-
-
-
-
-
-
-
1
-
1
-
1
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389605
Sloane
Metabolites of p-aminobenzoic ...
Mycobacterium tuberculosis
Biochim. Biophys. Acta
327
11-19
1973
-
-
-
-
-
-
2
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
3
-
3
-
-
-
-
-
-
3
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
389599
Gross
Reduction of aromatic acids to ...
Neurospora crassa
Eur. J. Biochem.
8
420-425
1969
-
-
-
-
-
-
2
5
-
-
1
-
-
1
-
-
1
-
-
1
1
1
11
-
1
1
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
5
-
-
1
-
-
-
-
1
-
1
1
1
11
-
1
1
-
-
2
-
-
-
-
-
-
-
-
-