BRENDA - Enzyme Database show
show all sequences of 1.1.1.91

Reduction of aromatic acids to aldehydes and alcohols in the cell-free system. 2. Purification and properties of aryl-alcohol: NADP-oxidoreductase from Neurospora crassa

Gross, G.G.; Zenk, M.H.; Eur. J. Biochem. 8, 420-425 (1969)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Iodosobenzoate
-
Neurospora crassa
p-chloromercuribenzoate
-
Neurospora crassa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADP+
-
Neurospora crassa
0.1
-
NADPH
aldehyde reduction
Neurospora crassa
0.5
-
4-methoxybenzaldehyde
-
Neurospora crassa
1.7
-
4-methoxybenzyl alcohol
-
Neurospora crassa
1.7
-
acetaldehyde
-
Neurospora crassa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Neurospora crassa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neurospora crassa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Neurospora crassa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Neurospora crassa
Storage Stability
Storage Stability
Organism
0C, potassium phosphate buffer pH 7.5, 1 mM mercaptoethanol, 2 weeks, no loss of activity
Neurospora crassa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methoxybenzyl alcohol + NADP+
i.e. anisic alcohol
389599
Neurospora crassa
4-methoxybenzaldehyde + NADPH
-
-
-
r
acetaldehyde + NADPH + H+
-
389599
Neurospora crassa
ethanol + NADP+
-
-
-
r
benzyl alcohol + NADP+
-
389599
Neurospora crassa
benzaldehyde + NADPH
-
-
-
r
cinnamic alcohol + NADP+
-
389599
Neurospora crassa
cinnamic aldehyde + NADPH
-
-
-
r
coniferyl alcohol + NADP+
i.e. 4-hydroxy-3-methoxycinnamic alcohol
389599
Neurospora crassa
coniferylaldehyde + NADPH + H+
-
-
-
r
additional information
broad substrate specificity towards both aromatic and to a lesser extent aliphatic aldehydes
389599
Neurospora crassa
?
-
-
-
-
n-butanol + NADP+
-
389599
Neurospora crassa
n-butanal + NADPH
-
-
-
r
n-pentanol + NADP+
-
389599
Neurospora crassa
n-pentanal + NADPH
-
-
-
r
n-propanol + NADP+
-
389599
Neurospora crassa
n-propanal + NADPH
-
-
-
r
salicyl alcohol + NADP+
i.e. p-hydroxybenzyl alcohol
389599
Neurospora crassa
p-hydroxybenzaldehyde + NADPH
-
-
-
r
veratryl alcohol + NADP+
i.e. 3,4-dimethoxybenzyl alcohol
389599
Neurospora crassa
veratraldehyde + NADPH
-
-
-
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Neurospora crassa
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
50
by raising the temperature of the incubation mixture a linear increase of the reaction up to 55C is noted, but the reaction over 45C stops after 60-75 s
Neurospora crassa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
aldehyde reduction
Neurospora crassa
8.8
-
alcohol oxidation
Neurospora crassa
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Neurospora crassa
NADPH
needs NADPH as cofactor, with NADH the reaction is less than 1% of the rate with NADPH
Neurospora crassa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Neurospora crassa
NADPH
needs NADPH as cofactor, with NADH the reaction is less than 1% of the rate with NADPH
Neurospora crassa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Iodosobenzoate
-
Neurospora crassa
p-chloromercuribenzoate
-
Neurospora crassa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADP+
-
Neurospora crassa
0.1
-
NADPH
aldehyde reduction
Neurospora crassa
0.5
-
4-methoxybenzaldehyde
-
Neurospora crassa
1.7
-
4-methoxybenzyl alcohol
-
Neurospora crassa
1.7
-
acetaldehyde
-
Neurospora crassa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
75000
-
gel filtration
Neurospora crassa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Neurospora crassa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Neurospora crassa
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Neurospora crassa
Storage Stability (protein specific)
Storage Stability
Organism
0C, potassium phosphate buffer pH 7.5, 1 mM mercaptoethanol, 2 weeks, no loss of activity
Neurospora crassa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-methoxybenzyl alcohol + NADP+
i.e. anisic alcohol
389599
Neurospora crassa
4-methoxybenzaldehyde + NADPH
-
-
-
r
acetaldehyde + NADPH + H+
-
389599
Neurospora crassa
ethanol + NADP+
-
-
-
r
benzyl alcohol + NADP+
-
389599
Neurospora crassa
benzaldehyde + NADPH
-
-
-
r
cinnamic alcohol + NADP+
-
389599
Neurospora crassa
cinnamic aldehyde + NADPH
-
-
-
r
coniferyl alcohol + NADP+
i.e. 4-hydroxy-3-methoxycinnamic alcohol
389599
Neurospora crassa
coniferylaldehyde + NADPH + H+
-
-
-
r
additional information
broad substrate specificity towards both aromatic and to a lesser extent aliphatic aldehydes
389599
Neurospora crassa
?
-
-
-
-
n-butanol + NADP+
-
389599
Neurospora crassa
n-butanal + NADPH
-
-
-
r
n-pentanol + NADP+
-
389599
Neurospora crassa
n-pentanal + NADPH
-
-
-
r
n-propanol + NADP+
-
389599
Neurospora crassa
n-propanal + NADPH
-
-
-
r
salicyl alcohol + NADP+
i.e. p-hydroxybenzyl alcohol
389599
Neurospora crassa
p-hydroxybenzaldehyde + NADPH
-
-
-
r
veratryl alcohol + NADP+
i.e. 3,4-dimethoxybenzyl alcohol
389599
Neurospora crassa
veratraldehyde + NADPH
-
-
-
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Neurospora crassa
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
50
by raising the temperature of the incubation mixture a linear increase of the reaction up to 55C is noted, but the reaction over 45C stops after 60-75 s
Neurospora crassa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
aldehyde reduction
Neurospora crassa
8.8
-
alcohol oxidation
Neurospora crassa
Other publictions for EC 1.1.1.91
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738738
Magomedova
Characterization of two novel ...
Cupriavidus necator, Cupriavidus necator H16
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221
78-90
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-
-
1
-
-
-
-
2
-
-
-
-
-
38
-
-
1
-
-
-
-
-
15
-
2
-
-
2
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
15
-
2
-
-
2
2
-
-
-
-
-
-
-
2
2
739695
Kovi
Global transcriptome changes i ...
Lolium perenne
Sci. Rep.
6
28702
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
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-
-
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1
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1
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-
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-
735498
Ying
Characterization of an allylic ...
Yokenella sp. WZY002
Appl. Environ. Microbiol.
80
2399-2409
2014
3
-
1
-
-
-
7
6
-
3
1
-
4
1
-
-
1
-
-
-
-
-
13
1
2
-
1
6
2
-
-
2
-
-
-
3
-
1
2
-
-
-
-
7
-
6
-
3
1
-
4
-
-
1
-
-
-
-
13
1
2
-
1
6
2
-
-
-
-
-
-
-
6
6
737956
Ken
-
Biochemical characterization o ...
Taiwanofungus camphoratus
Bot. Stud.
55
14
2014
-
-
1
-
-
-
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1
-
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1
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1
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1
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8
1
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1
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1
1
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3
-
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-
-
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1
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
8
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
737897
Lerchner
Crystallographic analysis and ...
Ralstonia sp., Ralstonia sp. DSMZ 6428
Biotechnol. Bioeng.
110
2803-2814
2013
-
-
-
1
3
-
-
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
12
-
-
-
-
-
-
-
-
12
12
724669
Yang
Cloning, expression and charac ...
Phanerochaete chrysosporium
BMC Microbiol.
12
126
2012
-
-
1
-
-
-
8
14
-
-
1
-
-
1
-
-
1
-
-
-
-
-
27
-
1
-
-
14
2
-
-
-
8
-
-
-
-
1
-
-
-
-
-
8
8
14
-
-
1
-
-
-
-
1
-
-
-
-
27
-
1
-
-
14
2
-
-
-
-
-
-
-
14
14
389607
Gutierrez
Anisaldehyde production and ar ...
Pleurotus pulmonarius
Appl. Environ. Microbiol.
60
1783-1788
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
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-
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-
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-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389606
Muheim
Purification and properties of ...
Phanerochaete chrysosporium
Eur. J. Biochem.
195
369-375
1991
-
-
-
-
-
-
5
-
-
-
2
-
-
1
-
-
1
-
-
-
-
2
11
1
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
2
-
-
-
-
1
-
-
-
2
11
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288179
Kato
-
Reductive transformation of be ...
Nocardia asteroides, Nocardia asteroides JCM3016
J. Ferment. Bioeng.
69
220-223
1990
-
-
-
-
-
-
-
-
-
1
-
3
-
2
-
-
-
-
-
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1
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3
-
1
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-
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-
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-
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-
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1
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3
-
-
-
-
-
-
1
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
389479
Dalkin
Stress responses in alfalfa (M ...
Medicago sativa
Plant Physiol.
92
440-446
1990
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
-
-
-
-
-
-
16
-
-
-
-
-
-
8
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389602
Rhodes
-
Changes in the activity of enz ...
Solanum lycopersicum
Phytochemistry
16
655-659
1977
-
-
-
-
-
-
-
-
-
-
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1
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1
1
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1
1
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-
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-
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389603
Mansell
-
Multiple forms and specificity ...
Picea abies
Phytochemistry
15
1849-1853
1976
-
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-
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1
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1
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1
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2
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2
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-
-
-
-
-
-
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1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389601
Rhodes
-
Enzymes involved in the reduct ...
Brassica napus subsp. rapifera
Phytochemistry
14
1235-1240
1975
-
-
-
-
-
-
-
4
-
-
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1
-
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1
-
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1
-
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1
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-
-
-
-
-
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2
-
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2
-
-
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4
-
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-
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-
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1
-
1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389598
Davies
-
Aromatic alcohol dehydrogenase ...
Solanum tuberosum
Phytochemistry
12
531-536
1973
-
-
-
-
-
-
-
21
-
-
-
-
-
1
-
-
1
-
-
1
-
1
15
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
21
-
-
-
-
-
-
-
1
-
1
-
1
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389605
Sloane
Metabolites of p-aminobenzoic ...
Mycobacterium tuberculosis
Biochim. Biophys. Acta
327
11-19
1973
-
-
-
-
-
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2
2
-
-
-
-
-
1
-
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1
-
-
-
-
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2
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1
3
-
3
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3
-
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-
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2
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2
-
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1
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2
-
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-
-
1
3
-
-
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-
-
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-
389599
Gross
Reduction of aromatic acids to ...
Neurospora crassa
Eur. J. Biochem.
8
420-425
1969
-
-
-
-
-
-
2
5
-
-
1
-
-
1
-
-
1
-
-
1
1
1
11
-
1
1
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
5
-
-
1
-
-
-
-
1
-
1
1
1
11
-
1
1
-
-
2
-
-
-
-
-
-
-
-
-