BRENDA - Enzyme Database show
show all sequences of 1.1.1.90

Characterization of an allylic/benzyl alcohol dehydrogenase from Yokenella sp. strain WZY002, an organism potentially useful for the synthesis of alpha,beta-unsaturated alcohols from allylic aldehydes and ketones

Ying, X.; Wang, Y.; Xiong, B.; Wu, T.; Xie, L.; Yu, M.; Wang, Z.; Appl. Environ. Microbiol. 80, 2399-2409 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Yokenella sp. WZY002
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
-
Yokenella sp. WZY002
Cu2+
-
Yokenella sp. WZY002
Zn2+
-
Yokenella sp. WZY002
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
dependent on
Yokenella sp. WZY002
Organic Solvent Stability
Organic Solvent
Commentary
Organism
DMSO
the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide
Yokenella sp. WZY002
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Yokenella sp. WZY002
W6CX26
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + NADPH + H+
highest reductive activity with benzaldehyde
735498
Yokenella sp. WZY002
benzyl alcohol + NADP+
-
-
-
r
benzyl alcohol + NADP+
-
735498
Yokenella sp. WZY002
benzaldehyde + NADPH + H+
-
-
-
r
crotonaldehyde + NADPH + H+
-
735498
Yokenella sp. WZY002
crotonyl alcohol + NADP+
-
-
-
r
crotonyl alcohol + NADP+
highest oxidative activity with crotyl alcohol
735498
Yokenella sp. WZY002
crotonaldehyde + NADPH + H+
-
-
-
r
additional information
no activity with NADH or NAD+
735498
Yokenella sp. WZY002
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 36411, calculated from amino acid sequence
Yokenella sp. WZY002
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
for crotonyl alcohol oxidation
Yokenella sp. WZY002
65
-
for crotonaldehyde reduction
Yokenella sp. WZY002
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55C
Yokenella sp. WZY002
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
for crotonaldehyde reduction
Yokenella sp. WZY002
8
-
for crotonyl alcohol oxidation
Yokenella sp. WZY002
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Yokenella sp. WZY002
NADPH
-
Yokenella sp. WZY002
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Yokenella sp. WZY002
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Yokenella sp. WZY002
NADPH
-
Yokenella sp. WZY002
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
-
Yokenella sp. WZY002
Cu2+
-
Yokenella sp. WZY002
Zn2+
-
Yokenella sp. WZY002
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
dependent on
Yokenella sp. WZY002
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
DMSO
the enzyme retains 87.5% of the initial activity after 24 h of incubation with 20% (v/v) dimethyl sulfoxide
Yokenella sp. WZY002
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + NADPH + H+
highest reductive activity with benzaldehyde
735498
Yokenella sp. WZY002
benzyl alcohol + NADP+
-
-
-
r
benzyl alcohol + NADP+
-
735498
Yokenella sp. WZY002
benzaldehyde + NADPH + H+
-
-
-
r
crotonaldehyde + NADPH + H+
-
735498
Yokenella sp. WZY002
crotonyl alcohol + NADP+
-
-
-
r
crotonyl alcohol + NADP+
highest oxidative activity with crotyl alcohol
735498
Yokenella sp. WZY002
crotonaldehyde + NADPH + H+
-
-
-
r
additional information
no activity with NADH or NAD+
735498
Yokenella sp. WZY002
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36411, calculated from amino acid sequence
Yokenella sp. WZY002
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
for crotonyl alcohol oxidation
Yokenella sp. WZY002
65
-
for crotonaldehyde reduction
Yokenella sp. WZY002
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
the enzyme shows moderate thermostability with a half-life of 6.2 h at 55C
Yokenella sp. WZY002
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
for crotonaldehyde reduction
Yokenella sp. WZY002
8
-
for crotonyl alcohol oxidation
Yokenella sp. WZY002
Other publictions for EC 1.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735498
Ying
Characterization of an allylic ...
Yokenella sp. WZY002
Appl. Environ. Microbiol.
80
2399-2409
2014
-
-
1
-
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-
3
-
-
1
-
-
1
1
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5
1
2
-
1
-
2
-
-
2
-
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-
-
-
1
2
-
-
-
-
3
-
-
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1
-
-
1
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-
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-
-
5
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
724027
Uthoff
Purification and characterizat ...
Acinetobacter baylyi
Appl. Environ. Microbiol.
78
8743-8752
2012
-
-
1
-
-
-
2
2
-
7
2
-
-
1
-
-
1
-
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
7
2
-
-
-
-
1
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
1
1
-
2
2
724592
Nishimura
Characterization of two isozym ...
Streptomyces sp., Streptomyces sp. NL15-2K
Biosci. Biotechnol. Biochem.
75
1770-1777
2011
-
-
-
-
-
-
4
4
-
-
2
-
-
3
-
-
1
-
-
-
-
-
8
1
1
-
1
4
1
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1
3
-
-
-
-
-
-
3
-
-
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-
4
-
4
-
-
2
-
-
-
-
1
-
-
-
-
8
1
1
-
1
4
1
-
1
-
-
-
-
-
4
4
692723
Landete
Characterization of a benzyl a ...
Lactobacillus plantarum, Lactobacillus plantarum WCFS1, Lactobacillus plantarum WCFS1 NCIMB 8826
J. Agric. Food Chem.
56
4497-4503
2008
-
-
1
-
-
-
12
2
-
11
1
9
-
7
-
-
1
-
-
-
-
-
24
1
2
2
2
1
2
1
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
2
-
11
1
9
-
-
-
1
-
-
-
-
24
1
2
2
2
1
2
1
-
1
-
-
-
-
-
-
699815
Matsuzaki
Proteomic and metabolomic anal ...
Phanerochaete chrysosporium
J. Proteome Res.
7
2342-2350
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
667287
Peng
Characterization of four Rhodo ...
Rhodococcus erythropolis, Rhodococcus erythropolis PR4
Appl. Microbiol. Biotechnol.
71
824-832
2006
-
-
1
-
-
-
-
-
-
-
-
39
-
6
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
39
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667256
Garcia-Pena
Correlation of biological acti ...
Paecilomyces variotii, Paecilomyces variotii CBS115145
Appl. Environ. Microbiol.
71
4280-4285
2005
-
1
-
-
-
-
-
-
-
1
-
4
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670853
Shimizu
Metabolic regulation at the tr ...
Phanerochaete chrysosporium
Proteomics
5
3919-3931
2005
1
-
-
-
-
-
-
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
657089
Millanes
-
Histochemical detection of an ...
Evernia prunastri
Plant Physiol. Biochem.
41
786-791
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Cupriavidus necator, Pseudomonas putida
Appl. Environ. Microbiol.
68
4315-4321
2002
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655896
Ichinose
Molecular analysis of arylalco ...
Trametes versicolor
J. Basic Microbiol.
42
327-336
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389593
Curtis
Allylic or benzylic stabilizat ...
Acinetobacter calcoaceticus, Pseudomonas putida
Biochem. Biophys. Res. Commun.
259
220-223
1999
-
-
2
-
-
-
-
4
-
-
-
2
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
389595
Inoue
Proton transfer in benzyl alco ...
Pseudomonas putida
Biochemistry
37
3305-3311
1998
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389596
Biegert
Purification and properties of ...
Thauera sp., Thauera sp. K172
Arch. Microbiol.
163
418-423
1995
-
-
-
-
-
-
3
3
-
-
2
2
-
7
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
2
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
-
-
-
-
-
-
-
389594
Shaw
Kinetic studies on benzyl alco ...
Pseudomonas putida
J. Biol. Chem.
268
10842-10850
1993
-
-
-
-
-
-
2
4
-
6
1
2
-
5
-
-
-
1
-
-
-
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
-
3
-
4
-
12
1
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
-
-
10
-
-
2
3
-
2
-
-
1
-
-
-
1
1
9
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
10
-
-
2
3
-
-
-
1
-
-
1
1
9
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389581
Shaw
Purification and characterisat ...
Pseudomonas putida
Eur. J. Biochem.
191
705-714
1990
-
-
-
-
-
4
6
4
-
6
2
1
-
2
-
-
1
-
-
-
1
1
16
1
1
-
2
2
1
1
-
3
1
-
-
-
-
-
3
-
-
4
-
6
1
4
-
6
2
1
-
-
-
1
-
-
1
1
16
1
1
-
2
2
1
1
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
-
-
-
-
-
-
-
2
-
-
2
1
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
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389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
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653-661
1988
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11
12
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15
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7
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389590
Collins
-
Benzyl alcohol metabolism by P ...
Pseudomonas putida
Arch. Microbiol.
138
153-160
1984
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389591
Yamanaka
-
Identification and characteriz ...
Rhodoblastus acidophilus
Agric. Biol. Chem.
48
1161-1171
1984
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2
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389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
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16
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8
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8
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1
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16
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16
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16
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8
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1
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389585
Inouye
Molecular cloning of TOL genes ...
Pseudomonas putida
J. Bacteriol.
145
1137-1143
1981
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1
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4
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389588
Poh
Evidence for isofunctional enz ...
Pseudomonas alcaligenes
J. Bacteriol.
143
59-69
1980
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389589
Keat
The aromatic alcohol dehydroge ...
Pseudomonas putida
Biochem. J.
175
659-667
1978
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1
1
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3
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