BRENDA - Enzyme Database show
show all sequences of 1.1.1.90

Characterization of a benzyl alcohol dehydrogenase from Lactobacillus plantarum WCFS1

Landete, J.M.; Rodriguez, H.; de Las Rivas, B.; Munoz, R.; J. Agric. Food Chem. 56, 4497-4503 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene lp_3054, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109
Lactobacillus plantarum WCFS1
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
10 mM, 76% inhibition
Lactobacillus plantarum
2-mercaptoethanol
inhibits 76% at 10 mM
Lactobacillus plantarum WCFS1
Ag+
inhibits 58% at 10 mM
Lactobacillus plantarum WCFS1
AgNO3
10 mM, 58% inhibition
Lactobacillus plantarum
DMSO
10 mM, 20% inhibition
Lactobacillus plantarum
DMSO
inhibits 20% at 10 mM
Lactobacillus plantarum WCFS1
DTT
10 mM, 84% inhibition
Lactobacillus plantarum
DTT
inhibits 84% at 10 mM
Lactobacillus plantarum WCFS1
EDTA
10 mM, 22% inhibition
Lactobacillus plantarum
EDTA
inhibits 22% at 10 mM
Lactobacillus plantarum WCFS1
SDS
10 mM, 86% inhibition
Lactobacillus plantarum
SDS
inhibits 86% at 10 mM
Lactobacillus plantarum WCFS1
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
benzyl alcohol
-
Lactobacillus plantarum
0.23
-
benzyl alcohol
pH 5.0, 30C
Lactobacillus plantarum WCFS1
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
CaCl2
10 mM, 1.3fold increase in activity
Lactobacillus plantarum
CaCl2
increases the enzyme activity by 27% at 10 mM
Lactobacillus plantarum WCFS1
CdCl2
10 mM, 1.3fold increase in activity
Lactobacillus plantarum
CdCl2
increases the enzyme activity by 25% at 10 mM
Lactobacillus plantarum WCFS1
KBr
10 mM, 1.1fold increase in activity
Lactobacillus plantarum
KBr
increases the enzyme activity by 11% at 10 mM
Lactobacillus plantarum WCFS1
MgCl2
10 mM, 1.2fold increase in activity
Lactobacillus plantarum
MgCl2
increases the enzyme activity by 22% at 10 mM
Lactobacillus plantarum WCFS1
additional information
no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80
Lactobacillus plantarum WCFS1
Zn
zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved
Lactobacillus plantarum
Zn2+
dependent on, a catalytic zinc atom bound at the active site to the ligands Cys46, Asp49, His67, and Cys174 and a structural zinc atom bound to cysteine residues 97, 100, 103, and 111. All of the residues involved in zinc binding are conserved
Lactobacillus plantarum WCFS1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, recombinant enzyme, SDS-PAGE
Lactobacillus plantarum WCFS1
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
benzaldehyde + NAD(P)H + H+
-
-
?
benzyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1 NCIMB 8826
-
benzaldehyde + NAD(P)H + H+
-
-
?
cinnamyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
cinnamyl aldehyde + NAD(P)H + H+
-
-
?
cinnamyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1 NCIMB 8826
-
cinnamyl aldehyde + NAD(P)H + H+
-
-
?
coniferyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
coniferyl aldehyde + NAD(P)H + H+
-
-
?
coniferyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1 NCIMB 8826
-
coniferyl aldehyde + NAD(P)H + H+
-
-
?
geraniol + NAD(P)+
Lactobacillus plantarum WCFS1
-
geranial + NAD(P)H + H+
-
-
?
nerol + NAD(P)+
Lactobacillus plantarum WCFS1
-
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
-
?
phenethyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
phenylacetaldehyde + NAD(P)H + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus plantarum
-
strain WCFS1
-
Lactobacillus plantarum WCFS1
-
strain NCIMB 8826, gene gene lp_3054
-
Lactobacillus plantarum WCFS1 NCIMB 8826
-
strain NCIMB 8826, gene gene lp_3054
-
Purification (Commentary)
Commentary
Organism
recombinant lp_3054 from Escherichia coli strain JM109
Lactobacillus plantarum WCFS1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
benzaldehyde + NAD(P)H + H+
-
-
-
?
benzyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
benzaldehyde + NAD(P)H + H+
-
-
-
?
benzyl alcohol + NAD+
less active in the presence of NADP+ than in the presence of NAD+
692723
Lactobacillus plantarum
benzaldehyde + NADH
-
-
-
?
benzyl alcohol + NADP+
less active in the presence of NADP+ than in the presence of NAD+
692723
Lactobacillus plantarum
benzaldehyde + NADPH
-
-
-
?
cinnamyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD(P)+
67% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD(P)+
67% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD+
67% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
cinnamyl aldehyde + NADH + H+
-
-
-
?
coniferyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
coniferyl aldehyde + NAD(P)H + H+
-
-
-
?
coniferyl alcohol + NAD(P)+
59% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
coniferyl aldehyde + NAD(P)H + H+
-
-
-
?
coniferyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
coniferyl aldehyde + NAD(P)H + H+
-
-
-
?
coniferyl alcohol + NAD+
59% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
coniferyl aldehyde + NADH + H+
-
-
-
?
geraniol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
geranial + NAD(P)H + H+
-
-
-
?
geraniol + NAD(P)+
105% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
geranial + NAD(P)H + H+
-
-
-
?
geraniol + NAD+
105% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
(2Z)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
-
-
?
additional information
no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid
692723
Lactobacillus plantarum WCFS1
?
-
-
-
-
additional information
no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
?
-
-
-
-
nerol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
-
-
?
nerol + NAD(P)+
116% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
-
-
?
nerol + NAD+
116% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
(2E)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
-
-
?
phenethyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
phenethyl alcohol + NAD(P)+
73% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
phenethyl alcohol + NAD+
73% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
phenylacetaldehyde + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, recombinant enzyme, SDS-PAGE
Lactobacillus plantarum WCFS1
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Lactobacillus plantarum
30
-
-
Lactobacillus plantarum WCFS1
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
50
20C: about 60% of maximal activity, 50C: about 70% of maximal activity
Lactobacillus plantarum
25
50
activity range
Lactobacillus plantarum WCFS1
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
50
the purified recombinant enzyme remains quite stable
Lactobacillus plantarum WCFS1
70
-
purified recombinant enzyme, BADH activity significantly decreases to 16% of maximal activity
Lactobacillus plantarum WCFS1
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4
-
benzyl alcohol
pH 5.0, 30C
Lactobacillus plantarum WCFS1
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Lactobacillus plantarum
5
-
-
Lactobacillus plantarum WCFS1
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3.5
6
pH 3.5: about 60% of maximal activity, pH 6.0: about 45% of maximal activity
Lactobacillus plantarum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred cofactor compared to NADP+, all of the residues involved in cofactor binding are conserved. Residues Thr178 and Val203 interact with the nicotinamide ring, polypeptide backbone nitrogen atoms of residues Gly201 and Gly202 interact with an oxygen of the phosphate proximal to the nicotinamide group, and His47 binds the diphosphate of the NAD+ coenzyme. An Asp residue determines the specificity for NAD+
Lactobacillus plantarum WCFS1
NADP+
preferred cofactor is NAD+, all of the residues involved in cofactor binding are conserved. An Asp residue determines the specificity for NAD+
Lactobacillus plantarum WCFS1
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Lactobacillus plantarum WCFS1
sequence calculation
-
5.04
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lp_3054, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109
Lactobacillus plantarum WCFS1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred cofactor compared to NADP+, all of the residues involved in cofactor binding are conserved. Residues Thr178 and Val203 interact with the nicotinamide ring, polypeptide backbone nitrogen atoms of residues Gly201 and Gly202 interact with an oxygen of the phosphate proximal to the nicotinamide group, and His47 binds the diphosphate of the NAD+ coenzyme. An Asp residue determines the specificity for NAD+
Lactobacillus plantarum WCFS1
NADP+
preferred cofactor is NAD+, all of the residues involved in cofactor binding are conserved. An Asp residue determines the specificity for NAD+
Lactobacillus plantarum WCFS1
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
10 mM, 76% inhibition
Lactobacillus plantarum
2-mercaptoethanol
inhibits 76% at 10 mM
Lactobacillus plantarum WCFS1
Ag+
inhibits 58% at 10 mM
Lactobacillus plantarum WCFS1
AgNO3
10 mM, 58% inhibition
Lactobacillus plantarum
DMSO
10 mM, 20% inhibition
Lactobacillus plantarum
DMSO
inhibits 20% at 10 mM
Lactobacillus plantarum WCFS1
DTT
10 mM, 84% inhibition
Lactobacillus plantarum
DTT
inhibits 84% at 10 mM
Lactobacillus plantarum WCFS1
EDTA
10 mM, 22% inhibition
Lactobacillus plantarum
EDTA
inhibits 22% at 10 mM
Lactobacillus plantarum WCFS1
SDS
10 mM, 86% inhibition
Lactobacillus plantarum
SDS
inhibits 86% at 10 mM
Lactobacillus plantarum WCFS1
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.23
-
benzyl alcohol
-
Lactobacillus plantarum
0.23
-
benzyl alcohol
pH 5.0, 30C
Lactobacillus plantarum WCFS1
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
CaCl2
10 mM, 1.3fold increase in activity
Lactobacillus plantarum
CaCl2
increases the enzyme activity by 27% at 10 mM
Lactobacillus plantarum WCFS1
CdCl2
10 mM, 1.3fold increase in activity
Lactobacillus plantarum
CdCl2
increases the enzyme activity by 25% at 10 mM
Lactobacillus plantarum WCFS1
KBr
10 mM, 1.1fold increase in activity
Lactobacillus plantarum
KBr
increases the enzyme activity by 11% at 10 mM
Lactobacillus plantarum WCFS1
MgCl2
10 mM, 1.2fold increase in activity
Lactobacillus plantarum
MgCl2
increases the enzyme activity by 22% at 10 mM
Lactobacillus plantarum WCFS1
additional information
no effects on the enzyme activity by KCl, NaCl, and NiCl2, and by 10 mM urea and Tween-80
Lactobacillus plantarum WCFS1
Zn
zinc-dependent alcohol dehydrogenase. All of the residues involved in zinc binding are conserved
Lactobacillus plantarum
Zn2+
dependent on, a catalytic zinc atom bound at the active site to the ligands Cys46, Asp49, His67, and Cys174 and a structural zinc atom bound to cysteine residues 97, 100, 103, and 111. All of the residues involved in zinc binding are conserved
Lactobacillus plantarum WCFS1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
x * 40000, recombinant enzyme, SDS-PAGE
Lactobacillus plantarum WCFS1
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
benzaldehyde + NAD(P)H + H+
-
-
?
benzyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1 NCIMB 8826
-
benzaldehyde + NAD(P)H + H+
-
-
?
cinnamyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
cinnamyl aldehyde + NAD(P)H + H+
-
-
?
cinnamyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1 NCIMB 8826
-
cinnamyl aldehyde + NAD(P)H + H+
-
-
?
coniferyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
coniferyl aldehyde + NAD(P)H + H+
-
-
?
coniferyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1 NCIMB 8826
-
coniferyl aldehyde + NAD(P)H + H+
-
-
?
geraniol + NAD(P)+
Lactobacillus plantarum WCFS1
-
geranial + NAD(P)H + H+
-
-
?
nerol + NAD(P)+
Lactobacillus plantarum WCFS1
-
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
-
?
phenethyl alcohol + NAD(P)+
Lactobacillus plantarum WCFS1
-
phenylacetaldehyde + NAD(P)H + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant lp_3054 from Escherichia coli strain JM109
Lactobacillus plantarum WCFS1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
benzaldehyde + NAD(P)H + H+
-
-
-
?
benzyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
benzaldehyde + NAD(P)H + H+
-
-
-
?
benzyl alcohol + NAD+
less active in the presence of NADP+ than in the presence of NAD+
692723
Lactobacillus plantarum
benzaldehyde + NADH
-
-
-
?
benzyl alcohol + NADP+
less active in the presence of NADP+ than in the presence of NAD+
692723
Lactobacillus plantarum
benzaldehyde + NADPH
-
-
-
?
cinnamyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD(P)+
67% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD(P)+
67% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
cinnamyl aldehyde + NAD(P)H + H+
-
-
-
?
cinnamyl alcohol + NAD+
67% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
cinnamyl aldehyde + NADH + H+
-
-
-
?
coniferyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
coniferyl aldehyde + NAD(P)H + H+
-
-
-
?
coniferyl alcohol + NAD(P)+
59% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
coniferyl aldehyde + NAD(P)H + H+
-
-
-
?
coniferyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
coniferyl aldehyde + NAD(P)H + H+
-
-
-
?
coniferyl alcohol + NAD+
59% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
coniferyl aldehyde + NADH + H+
-
-
-
?
geraniol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
geranial + NAD(P)H + H+
-
-
-
?
geraniol + NAD(P)+
105% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
geranial + NAD(P)H + H+
-
-
-
?
geraniol + NAD+
105% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
(2Z)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
-
-
?
additional information
no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid
692723
Lactobacillus plantarum WCFS1
?
-
-
-
-
additional information
no activity with tyrosol, tryptophol, pyrogallol, catechol, phloroglucinol, ethanol, p-coumaric acid, and caffeic acid
692723
Lactobacillus plantarum WCFS1 NCIMB 8826
?
-
-
-
-
nerol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
-
-
?
nerol + NAD(P)+
116% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
(2E)-3,7-dimethylocta-2,6-dienal + NAD(P)H + H+
-
-
-
?
nerol + NAD+
116% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
(2E)-3,7-dimethylocta-2,6-dienal + NADH + H+
-
-
-
?
phenethyl alcohol + NAD(P)+
-
692723
Lactobacillus plantarum WCFS1
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
phenethyl alcohol + NAD(P)+
73% activity compared to activity with benzyl alcohol
692723
Lactobacillus plantarum WCFS1
phenylacetaldehyde + NAD(P)H + H+
-
-
-
?
phenethyl alcohol + NAD+
73% of the activity with benzyl alcohol
692723
Lactobacillus plantarum
phenylacetaldehyde + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, recombinant enzyme, SDS-PAGE
Lactobacillus plantarum WCFS1
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
-
Lactobacillus plantarum
30
-
-
Lactobacillus plantarum WCFS1
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
20
50
20C: about 60% of maximal activity, 50C: about 70% of maximal activity
Lactobacillus plantarum
25
50
activity range
Lactobacillus plantarum WCFS1
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
50
the purified recombinant enzyme remains quite stable
Lactobacillus plantarum WCFS1
70
-
purified recombinant enzyme, BADH activity significantly decreases to 16% of maximal activity
Lactobacillus plantarum WCFS1
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4
-
benzyl alcohol
pH 5.0, 30C
Lactobacillus plantarum WCFS1
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Lactobacillus plantarum
5
-
-
Lactobacillus plantarum WCFS1
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3.5
6
pH 3.5: about 60% of maximal activity, pH 6.0: about 45% of maximal activity
Lactobacillus plantarum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Lactobacillus plantarum WCFS1
sequence calculation
-
5.04
Other publictions for EC 1.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735498
Ying
Characterization of an allylic ...
Yokenella sp. WZY002
Appl. Environ. Microbiol.
80
2399-2409
2014
-
-
1
-
-
-
3
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
5
1
2
-
1
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
3
-
-
-
1
-
-
1
-
-
-
-
-
-
-
5
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
724027
Uthoff
Purification and characterizat ...
Acinetobacter baylyi
Appl. Environ. Microbiol.
78
8743-8752
2012
-
-
1
-
-
-
2
2
-
7
2
-
-
1
-
-
1
-
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
7
2
-
-
-
-
1
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
1
1
-
2
2
724592
Nishimura
Characterization of two isozym ...
Streptomyces sp., Streptomyces sp. NL15-2K
Biosci. Biotechnol. Biochem.
75
1770-1777
2011
-
-
-
-
-
-
4
4
-
-
2
-
-
3
-
-
1
-
-
-
-
-
8
1
1
-
1
4
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
4
-
4
-
-
2
-
-
-
-
1
-
-
-
-
8
1
1
-
1
4
1
-
1
-
-
-
-
-
4
4
692723
Landete
Characterization of a benzyl a ...
Lactobacillus plantarum, Lactobacillus plantarum WCFS1, Lactobacillus plantarum WCFS1 NCIMB 8826
J. Agric. Food Chem.
56
4497-4503
2008
-
-
1
-
-
-
12
2
-
11
1
9
-
7
-
-
1
-
-
-
-
-
24
1
2
2
2
1
2
1
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
2
-
11
1
9
-
-
-
1
-
-
-
-
24
1
2
2
2
1
2
1
-
1
-
-
-
-
-
-
699815
Matsuzaki
Proteomic and metabolomic anal ...
Phanerochaete chrysosporium
J. Proteome Res.
7
2342-2350
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
667287
Peng
Characterization of four Rhodo ...
Rhodococcus erythropolis, Rhodococcus erythropolis PR4
Appl. Microbiol. Biotechnol.
71
824-832
2006
-
-
1
-
-
-
-
-
-
-
-
39
-
6
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
39
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667256
Garcia-Pena
Correlation of biological acti ...
Paecilomyces variotii, Paecilomyces variotii CBS115145
Appl. Environ. Microbiol.
71
4280-4285
2005
-
1
-
-
-
-
-
-
-
1
-
4
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670853
Shimizu
Metabolic regulation at the tr ...
Phanerochaete chrysosporium
Proteomics
5
3919-3931
2005
1
-
-
-
-
-
-
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
657089
Millanes
-
Histochemical detection of an ...
Evernia prunastri
Plant Physiol. Biochem.
41
786-791
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Cupriavidus necator, Pseudomonas putida
Appl. Environ. Microbiol.
68
4315-4321
2002
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655896
Ichinose
Molecular analysis of arylalco ...
Trametes versicolor
J. Basic Microbiol.
42
327-336
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389593
Curtis
Allylic or benzylic stabilizat ...
Acinetobacter calcoaceticus, Pseudomonas putida
Biochem. Biophys. Res. Commun.
259
220-223
1999
-
-
2
-
-
-
-
4
-
-
-
2
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
389595
Inoue
Proton transfer in benzyl alco ...
Pseudomonas putida
Biochemistry
37
3305-3311
1998
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389596
Biegert
Purification and properties of ...
Thauera sp., Thauera sp. K172
Arch. Microbiol.
163
418-423
1995
-
-
-
-
-
-
3
3
-
-
2
2
-
7
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
2
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
-
-
-
-
-
-
-
389594
Shaw
Kinetic studies on benzyl alco ...
Pseudomonas putida
J. Biol. Chem.
268
10842-10850
1993
-
-
-
-
-
-
2
4
-
6
1
2
-
5
-
-
-
1
-
-
-
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
-
3
-
4
-
12
1
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
-
-
10
-
-
2
3
-
2
-
-
1
-
-
-
1
1
9
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
10
-
-
2
3
-
-
-
1
-
-
1
1
9
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389581
Shaw
Purification and characterisat ...
Pseudomonas putida
Eur. J. Biochem.
191
705-714
1990
-
-
-
-
-
4
6
4
-
6
2
1
-
2
-
-
1
-
-
-
1
1
16
1
1
-
2
2
1
1
-
3
1
-
-
-
-
-
3
-
-
4
-
6
1
4
-
6
2
1
-
-
-
1
-
-
1
1
16
1
1
-
2
2
1
1
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
-
-
-
-
-
-
-
2
-
-
2
1
-
3
-
-
1
-
-
-
1
-
1
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
255
653-661
1988
-
-
-
-
-
-
11
12
-
-
-
1
-
2
-
-
-
-
-
-
-
-
15
-
-
-
-
7
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
11
-
12
-
-
-
1
-
-
-
-
-
-
-
-
15
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
389590
Collins
-
Benzyl alcohol metabolism by P ...
Pseudomonas putida
Arch. Microbiol.
138
153-160
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389591
Yamanaka
-
Identification and characteriz ...
Rhodoblastus acidophilus
Agric. Biol. Chem.
48
1161-1171
1984
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
-
-
-
-
-
-
16
-
-
-
-
-
-
8
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
16
-
-
-
-
-
-
16
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389585
Inouye
Molecular cloning of TOL genes ...
Pseudomonas putida
J. Bacteriol.
145
1137-1143
1981
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389588
Poh
Evidence for isofunctional enz ...
Pseudomonas alcaligenes
J. Bacteriol.
143
59-69
1980
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389589
Keat
The aromatic alcohol dehydroge ...
Pseudomonas putida
Biochem. J.
175
659-667
1978
-
-
-
-
-
1
1
-
-
-
3
-
-
1
-
-
1
-
-
-
1
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
3
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-