BRENDA - Enzyme Database show
show all sequences of 1.1.1.90

Correlation of biological activity and reactor performance in biofiltration of toluene with the fungus Paecilomyces variotii CBS115145

Garcia-Pena, I.; Hernandez, S.; Auria, R.; Revah, S.; Appl. Environ. Microbiol. 71, 4280-4285 (2005)

Data extracted from this reference:

Application
Application
Commentary
Organism
degradation
the enzyme is active in toluene degradtion in a reactor, containing the fungus Paecilomyces variotii strain CBS115145, for biofiltration of toluene
Paecilomyces variotii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Paecilomyces variotii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NAD(P)H + H+
Paecilomyces variotii
-
benzyl alcohol + NAD(P)+
-
-
?
benzaldehyde + NAD(P)H + H+
Paecilomyces variotii CBS115145
-
benzyl alcohol + NAD(P)+
-
-
?
additional information
Paecilomyces variotii
the enzyme is involved in the toluene metabolic pathway, overview
?
-
-
-
additional information
Paecilomyces variotii CBS115145
the enzyme is involved in the toluene metabolic pathway, overview
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Paecilomyces variotii
-
strain CBS115145
-
Paecilomyces variotii CBS115145
-
strain CBS115145
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
liquid culture grown on toluene or intermediates of the toluene metabolic pathway, growth efficiency overview
Paecilomyces variotii
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0002
-
biofilter culture cell extract, cofactor NADP+
Paecilomyces variotii
0.016
-
liquid culture cell extract, cofactor NAD+
Paecilomyces variotii
0.036
-
biofilter culture cell extract, cofactor NAD+
Paecilomyces variotii
0.05
-
liquid culture cell extract, cofactor NADP+
Paecilomyces variotii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + NAD(P)H + H+
-
667256
Paecilomyces variotii
benzyl alcohol + NAD(P)+
-
-
-
?
benzaldehyde + NAD(P)H + H+
-
667256
Paecilomyces variotii CBS115145
benzyl alcohol + NAD(P)+
-
-
-
?
additional information
the enzyme is involved in the toluene metabolic pathway, overview
667256
Paecilomyces variotii
?
-
-
-
-
additional information
the enzyme is involved in the toluene metabolic pathway, overview
667256
Paecilomyces variotii CBS115145
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Paecilomyces variotii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Paecilomyces variotii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred cofactor
Paecilomyces variotii
NADP+
-
Paecilomyces variotii
Application (protein specific)
Application
Commentary
Organism
degradation
the enzyme is active in toluene degradtion in a reactor, containing the fungus Paecilomyces variotii strain CBS115145, for biofiltration of toluene
Paecilomyces variotii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred cofactor
Paecilomyces variotii
NADP+
-
Paecilomyces variotii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Paecilomyces variotii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzaldehyde + NAD(P)H + H+
Paecilomyces variotii
-
benzyl alcohol + NAD(P)+
-
-
?
benzaldehyde + NAD(P)H + H+
Paecilomyces variotii CBS115145
-
benzyl alcohol + NAD(P)+
-
-
?
additional information
Paecilomyces variotii
the enzyme is involved in the toluene metabolic pathway, overview
?
-
-
-
additional information
Paecilomyces variotii CBS115145
the enzyme is involved in the toluene metabolic pathway, overview
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
liquid culture grown on toluene or intermediates of the toluene metabolic pathway, growth efficiency overview
Paecilomyces variotii
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0002
-
biofilter culture cell extract, cofactor NADP+
Paecilomyces variotii
0.016
-
liquid culture cell extract, cofactor NAD+
Paecilomyces variotii
0.036
-
biofilter culture cell extract, cofactor NAD+
Paecilomyces variotii
0.05
-
liquid culture cell extract, cofactor NADP+
Paecilomyces variotii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzaldehyde + NAD(P)H + H+
-
667256
Paecilomyces variotii
benzyl alcohol + NAD(P)+
-
-
-
?
benzaldehyde + NAD(P)H + H+
-
667256
Paecilomyces variotii CBS115145
benzyl alcohol + NAD(P)+
-
-
-
?
additional information
the enzyme is involved in the toluene metabolic pathway, overview
667256
Paecilomyces variotii
?
-
-
-
-
additional information
the enzyme is involved in the toluene metabolic pathway, overview
667256
Paecilomyces variotii CBS115145
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Paecilomyces variotii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Paecilomyces variotii
Other publictions for EC 1.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735498
Ying
Characterization of an allylic ...
Yokenella sp. WZY002
Appl. Environ. Microbiol.
80
2399-2409
2014
-
-
1
-
-
-
3
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
5
1
2
-
1
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
3
-
-
-
1
-
-
1
-
-
-
-
-
-
-
5
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
724027
Uthoff
Purification and characterizat ...
Acinetobacter baylyi
Appl. Environ. Microbiol.
78
8743-8752
2012
-
-
1
-
-
-
2
2
-
7
2
-
-
1
-
-
1
-
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
7
2
-
-
-
-
1
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
1
1
-
2
2
724592
Nishimura
Characterization of two isozym ...
Streptomyces sp., Streptomyces sp. NL15-2K
Biosci. Biotechnol. Biochem.
75
1770-1777
2011
-
-
-
-
-
-
4
4
-
-
2
-
-
3
-
-
1
-
-
-
-
-
8
1
1
-
1
4
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
4
-
4
-
-
2
-
-
-
-
1
-
-
-
-
8
1
1
-
1
4
1
-
1
-
-
-
-
-
4
4
692723
Landete
Characterization of a benzyl a ...
Lactobacillus plantarum, Lactobacillus plantarum WCFS1, Lactobacillus plantarum WCFS1 NCIMB 8826
J. Agric. Food Chem.
56
4497-4503
2008
-
-
1
-
-
-
12
2
-
11
1
9
-
7
-
-
1
-
-
-
-
-
24
1
2
2
2
1
2
1
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
2
-
11
1
9
-
-
-
1
-
-
-
-
24
1
2
2
2
1
2
1
-
1
-
-
-
-
-
-
699815
Matsuzaki
Proteomic and metabolomic anal ...
Phanerochaete chrysosporium
J. Proteome Res.
7
2342-2350
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
667287
Peng
Characterization of four Rhodo ...
Rhodococcus erythropolis, Rhodococcus erythropolis PR4
Appl. Microbiol. Biotechnol.
71
824-832
2006
-
-
1
-
-
-
-
-
-
-
-
39
-
6
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
39
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667256
Garcia-Pena
Correlation of biological acti ...
Paecilomyces variotii, Paecilomyces variotii CBS115145
Appl. Environ. Microbiol.
71
4280-4285
2005
-
1
-
-
-
-
-
-
-
1
-
4
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670853
Shimizu
Metabolic regulation at the tr ...
Phanerochaete chrysosporium
Proteomics
5
3919-3931
2005
1
-
-
-
-
-
-
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
657089
Millanes
-
Histochemical detection of an ...
Evernia prunastri
Plant Physiol. Biochem.
41
786-791
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Cupriavidus necator, Pseudomonas putida
Appl. Environ. Microbiol.
68
4315-4321
2002
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655896
Ichinose
Molecular analysis of arylalco ...
Trametes versicolor
J. Basic Microbiol.
42
327-336
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389593
Curtis
Allylic or benzylic stabilizat ...
Acinetobacter calcoaceticus, Pseudomonas putida
Biochem. Biophys. Res. Commun.
259
220-223
1999
-
-
2
-
-
-
-
4
-
-
-
2
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
389595
Inoue
Proton transfer in benzyl alco ...
Pseudomonas putida
Biochemistry
37
3305-3311
1998
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389596
Biegert
Purification and properties of ...
Thauera sp., Thauera sp. K172
Arch. Microbiol.
163
418-423
1995
-
-
-
-
-
-
3
3
-
-
2
2
-
7
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
2
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
-
-
-
-
-
-
-
389594
Shaw
Kinetic studies on benzyl alco ...
Pseudomonas putida
J. Biol. Chem.
268
10842-10850
1993
-
-
-
-
-
-
2
4
-
6
1
2
-
5
-
-
-
1
-
-
-
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
-
3
-
4
-
12
1
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
-
-
10
-
-
2
3
-
2
-
-
1
-
-
-
1
1
9
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
10
-
-
2
3
-
-
-
1
-
-
1
1
9
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389581
Shaw
Purification and characterisat ...
Pseudomonas putida
Eur. J. Biochem.
191
705-714
1990
-
-
-
-
-
4
6
4
-
6
2
1
-
2
-
-
1
-
-
-
1
1
16
1
1
-
2
2
1
1
-
3
1
-
-
-
-
-
3
-
-
4
-
6
1
4
-
6
2
1
-
-
-
1
-
-
1
1
16
1
1
-
2
2
1
1
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
-
-
-
-
-
-
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2
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2
1
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3
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1
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1
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1
1
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-
2
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-
2
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2
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-
-
-
2
-
-
2
1
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-
-
1
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-
1
-
1
1
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-
-
-
2
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-
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389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
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653-661
1988
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11
12
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1
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2
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15
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7
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2
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2
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11
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12
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1
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15
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7
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389590
Collins
-
Benzyl alcohol metabolism by P ...
Pseudomonas putida
Arch. Microbiol.
138
153-160
1984
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-
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1
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2
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2
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389591
Yamanaka
-
Identification and characteriz ...
Rhodoblastus acidophilus
Agric. Biol. Chem.
48
1161-1171
1984
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-
-
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1
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1
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1
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1
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2
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2
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2
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1
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1
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1
-
2
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-
389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
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16
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8
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8
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1
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16
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16
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16
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8
-
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1
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389585
Inouye
Molecular cloning of TOL genes ...
Pseudomonas putida
J. Bacteriol.
145
1137-1143
1981
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1
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4
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2
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1
2
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389588
Poh
Evidence for isofunctional enz ...
Pseudomonas alcaligenes
J. Bacteriol.
143
59-69
1980
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1
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2
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389589
Keat
The aromatic alcohol dehydroge ...
Pseudomonas putida
Biochem. J.
175
659-667
1978
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1
1
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3
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1
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1
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1
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1
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1
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2
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2
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1
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1
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3
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1
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1
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1
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1
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