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Literature summary for 1.1.1.90 extracted from
- Biotransformation of eugenol to ferulic acid by a recombinant strain of Ralstonia eutropha H16 (2002), Appl. Environ. Microbiol., 68, 4315-4321.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | biotechnological production of vanillin | Pseudomonas putida |
| synthesis | biotechnological production of vanillin | Cupriavidus necator |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli XL1-Blue | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
recombinant strain H16(pBBR1-JO2ehyABcalAcalB) | - |
| Pseudomonas putida | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.22 | - |
recombinant strain | Cupriavidus necator |
| 0.24 | - |
comparison to recombinant enzymes | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl alcohol + NAD+ | biotransformation of eugenol to ferulic acid | Pseudomonas putida | coniferyl aldehyde + NADH + H+ | - |
? |  |
| coniferyl alcohol + NAD+ | biotransformation of eugenol to ferulic acid | Cupriavidus necator | coniferyl aldehyde + NADH + H+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas putida | |
| NAD+ | - |
Cupriavidus necator | |
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Release 2023.1 (January 2023)
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