BRENDA - Enzyme Database show
show all sequences of 1.1.1.90

Purification and properties of benzyl alcohol dehydrogenase from a denitrifying Thauera sp

Biegert, T.; Altenschmidt, U.; Eckerskorn, C.; Fuchs, G.; Arch. Microbiol. 163, 418-423 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-(2-nitrobenzoate)
-
Thauera sp.
additional information
no inhibition by O2, EDTA, 2,2'bipyridy, pyrazole
Thauera sp.
p-chloromercuribenzoate
-
Thauera sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NAD+
-
Thauera sp.
0.3
-
4-Hydroxybenzyl alcohol
-
Thauera sp.
0.32
-
benzyl alcohol
-
Thauera sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
4 * 40000, SDS-PAGE
Thauera sp.
160000
-
gel filtration
Thauera sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzyl alcohol + NAD+
Thauera sp.
-
benzaldehyde + NADH
-
Thauera sp.
r
benzyl alcohol + NAD+
Thauera sp. K172
-
benzaldehyde + NADH
-
Thauera sp. K172
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thauera sp.
-
strain K172
-
Thauera sp. K172
-
strain K172
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.106
-
-
Thauera sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-fluorobenzyl alcohol + NAD+
-
389596
Thauera sp.
2-fluorobenzaldehyde + NADH
-
-
-
r
2-fluorobenzyl alcohol + NAD+
-
389596
Thauera sp. K172
2-fluorobenzaldehyde + NADH
-
-
-
r
4-hydroxybenzyl alcohol + NAD+
125% of benzyl alcohol activity
389596
Thauera sp.
4-hydroxybenzaldehyde + NADH
-
-
-
r
4-hydroxybenzyl alcohol + NAD+
125% of benzyl alcohol activity
389596
Thauera sp. K172
4-hydroxybenzaldehyde + NADH
-
-
-
r
benzyl alcohol + NAD+
-
389596
Thauera sp.
benzaldehyde + NADH
-
389596
Thauera sp.
r
benzyl alcohol + NAD+
-
389596
Thauera sp. K172
benzaldehyde + NADH
-
389596
Thauera sp. K172
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 40000, SDS-PAGE
Thauera sp.
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
85
-
benzyl alcohol
-
Thauera sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of benzaldehyde
Thauera sp.
9.2
-
benzyl alcohol + NAD+
Thauera sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.7
7.6
less than half-maximal activity above and below, reduction of benzaldehyde
Thauera sp.
8.5
9.7
less than half-maximal activity above and below, oxidation of benzyl alcohol
Thauera sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Thauera sp.
NADH
-
Thauera sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Thauera sp.
NADH
-
Thauera sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis-(2-nitrobenzoate)
-
Thauera sp.
additional information
no inhibition by O2, EDTA, 2,2'bipyridy, pyrazole
Thauera sp.
p-chloromercuribenzoate
-
Thauera sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NAD+
-
Thauera sp.
0.3
-
4-Hydroxybenzyl alcohol
-
Thauera sp.
0.32
-
benzyl alcohol
-
Thauera sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
4 * 40000, SDS-PAGE
Thauera sp.
160000
-
gel filtration
Thauera sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzyl alcohol + NAD+
Thauera sp.
-
benzaldehyde + NADH
-
Thauera sp.
r
benzyl alcohol + NAD+
Thauera sp. K172
-
benzaldehyde + NADH
-
Thauera sp. K172
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.106
-
-
Thauera sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-fluorobenzyl alcohol + NAD+
-
389596
Thauera sp.
2-fluorobenzaldehyde + NADH
-
-
-
r
2-fluorobenzyl alcohol + NAD+
-
389596
Thauera sp. K172
2-fluorobenzaldehyde + NADH
-
-
-
r
4-hydroxybenzyl alcohol + NAD+
125% of benzyl alcohol activity
389596
Thauera sp.
4-hydroxybenzaldehyde + NADH
-
-
-
r
4-hydroxybenzyl alcohol + NAD+
125% of benzyl alcohol activity
389596
Thauera sp. K172
4-hydroxybenzaldehyde + NADH
-
-
-
r
benzyl alcohol + NAD+
-
389596
Thauera sp.
benzaldehyde + NADH
-
389596
Thauera sp.
r
benzyl alcohol + NAD+
-
389596
Thauera sp. K172
benzaldehyde + NADH
-
389596
Thauera sp. K172
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 40000, SDS-PAGE
Thauera sp.
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
85
-
benzyl alcohol
-
Thauera sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of benzaldehyde
Thauera sp.
9.2
-
benzyl alcohol + NAD+
Thauera sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.7
7.6
less than half-maximal activity above and below, reduction of benzaldehyde
Thauera sp.
8.5
9.7
less than half-maximal activity above and below, oxidation of benzyl alcohol
Thauera sp.
Other publictions for EC 1.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735498
Ying
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2399-2409
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-
-
1
-
-
-
3
-
-
1
-
-
1
1
-
-
-
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-
-
-
-
5
1
2
-
1
-
2
-
-
2
-
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1
2
-
-
-
-
3
-
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1
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1
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-
5
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
724027
Uthoff
Purification and characterizat ...
Acinetobacter baylyi
Appl. Environ. Microbiol.
78
8743-8752
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-
-
1
-
-
-
2
2
-
7
2
-
-
1
-
-
1
-
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
7
2
-
-
-
-
1
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
1
1
-
2
2
724592
Nishimura
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1770-1777
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-
-
-
-
-
-
4
4
-
-
2
-
-
3
-
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1
-
-
-
-
-
8
1
1
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1
4
1
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1
3
-
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3
-
-
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-
4
-
4
-
-
2
-
-
-
-
1
-
-
-
-
8
1
1
-
1
4
1
-
1
-
-
-
-
-
4
4
692723
Landete
Characterization of a benzyl a ...
Lactobacillus plantarum, Lactobacillus plantarum WCFS1, Lactobacillus plantarum WCFS1 NCIMB 8826
J. Agric. Food Chem.
56
4497-4503
2008
-
-
1
-
-
-
12
2
-
11
1
9
-
7
-
-
1
-
-
-
-
-
24
1
2
2
2
1
2
1
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
2
-
11
1
9
-
-
-
1
-
-
-
-
24
1
2
2
2
1
2
1
-
1
-
-
-
-
-
-
699815
Matsuzaki
Proteomic and metabolomic anal ...
Phanerochaete chrysosporium
J. Proteome Res.
7
2342-2350
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-
-
-
-
-
-
-
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1
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2
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1
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1
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1
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1
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1
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1
-
-
1
-
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-
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-
-
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-
-
1
1
-
-
-
667287
Peng
Characterization of four Rhodo ...
Rhodococcus erythropolis, Rhodococcus erythropolis PR4
Appl. Microbiol. Biotechnol.
71
824-832
2006
-
-
1
-
-
-
-
-
-
-
-
39
-
6
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
39
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667256
Garcia-Pena
Correlation of biological acti ...
Paecilomyces variotii, Paecilomyces variotii CBS115145
Appl. Environ. Microbiol.
71
4280-4285
2005
-
1
-
-
-
-
-
-
-
1
-
4
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
2
-
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-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670853
Shimizu
Metabolic regulation at the tr ...
Phanerochaete chrysosporium
Proteomics
5
3919-3931
2005
1
-
-
-
-
-
-
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
657089
Millanes
-
Histochemical detection of an ...
Evernia prunastri
Plant Physiol. Biochem.
41
786-791
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
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2
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-
2
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1
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1
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1
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2
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2
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-
-
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-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Cupriavidus necator, Pseudomonas putida
Appl. Environ. Microbiol.
68
4315-4321
2002
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
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2
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2
1
2
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2
-
2
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-
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-
-
-
-
-
655896
Ichinose
Molecular analysis of arylalco ...
Trametes versicolor
J. Basic Microbiol.
42
327-336
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
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1
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1
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1
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-
1
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-
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-
-
-
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-
-
-
-
-
389593
Curtis
Allylic or benzylic stabilizat ...
Acinetobacter calcoaceticus, Pseudomonas putida
Biochem. Biophys. Res. Commun.
259
220-223
1999
-
-
2
-
-
-
-
4
-
-
-
2
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
389595
Inoue
Proton transfer in benzyl alco ...
Pseudomonas putida
Biochemistry
37
3305-3311
1998
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
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-
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-
-
2
-
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2
-
2
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-
1
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-
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-
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-
2
-
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-
389596
Biegert
Purification and properties of ...
Thauera sp., Thauera sp. K172
Arch. Microbiol.
163
418-423
1995
-
-
-
-
-
-
3
3
-
-
2
2
-
7
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
2
-
-
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-
-
-
2
-
-
-
-
3
-
3
-
-
2
2
-
-
-
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-
-
1
-
6
1
-
-
-
1
2
2
-
-
-
-
-
-
-
-
389594
Shaw
Kinetic studies on benzyl alco ...
Pseudomonas putida
J. Biol. Chem.
268
10842-10850
1993
-
-
-
-
-
-
2
4
-
6
1
2
-
5
-
-
-
1
-
-
-
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
-
3
-
4
-
12
1
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
-
-
10
-
-
2
3
-
2
-
-
1
-
-
-
1
1
9
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
10
-
-
2
3
-
-
-
1
-
-
1
1
9
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389581
Shaw
Purification and characterisat ...
Pseudomonas putida
Eur. J. Biochem.
191
705-714
1990
-
-
-
-
-
4
6
4
-
6
2
1
-
2
-
-
1
-
-
-
1
1
16
1
1
-
2
2
1
1
-
3
1
-
-
-
-
-
3
-
-
4
-
6
1
4
-
6
2
1
-
-
-
1
-
-
1
1
16
1
1
-
2
2
1
1
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
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-
1
-
-
-
-
-
-
-
-
-
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3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
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-
-
-
-
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-
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-
-
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1
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-
-
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389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
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1
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3
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1
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9
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2
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2
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1
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1
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9
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389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
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-
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2
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2
1
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3
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1
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1
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1
1
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2
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2
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2
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2
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2
1
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1
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1
-
1
1
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2
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389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
255
653-661
1988
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11
12
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1
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2
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15
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7
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2
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2
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11
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12
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1
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15
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7
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389590
Collins
-
Benzyl alcohol metabolism by P ...
Pseudomonas putida
Arch. Microbiol.
138
153-160
1984
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1
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2
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2
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389591
Yamanaka
-
Identification and characteriz ...
Rhodoblastus acidophilus
Agric. Biol. Chem.
48
1161-1171
1984
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1
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1
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1
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1
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2
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2
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2
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1
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1
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1
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2
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389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
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16
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8
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8
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1
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16
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16
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16
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8
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1
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389585
Inouye
Molecular cloning of TOL genes ...
Pseudomonas putida
J. Bacteriol.
145
1137-1143
1981
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1
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4
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2
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1
2
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389588
Poh
Evidence for isofunctional enz ...
Pseudomonas alcaligenes
J. Bacteriol.
143
59-69
1980
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1
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2
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2
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389589
Keat
The aromatic alcohol dehydroge ...
Pseudomonas putida
Biochem. J.
175
659-667
1978
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1
1
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3
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1
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1
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1
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-
1
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-
1
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2
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-
2
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1
-
1
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3
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1
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1
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1
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1
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