BRENDA - Enzyme Database show
show all sequences of 1.1.1.90

Kinetic studies on benzyl alcohol dehydrogenase encoded by TOL plasmid pWWO. A member of the zinc-containing long chain alcohol dehydrogenase family

Shaw, J.P.; Rekik, M.; Schwager, F.; Harayama, S.; J. Biol. Chem. 268, 10842-10850 (1993)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
;
Pseudomonas putida
additional information
product inhibition
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
0.065
benzaldehyde
-
Pseudomonas putida
0.145
0.155
benzyl alcohol
-
Pseudomonas putida
0.195
0.225
NAD+
-
Pseudomonas putida
0.46
1.49
NADH
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KBr
activity nearly doubled in presence of salts; activity nearly doubled in presence of salts
Pseudomonas putida
KCl
; activity nearly doubled in presence of salts
Pseudomonas putida
KNO3
activity nearly doubled in presence of salts; activity nearly doubled in presence of salts
Pseudomonas putida
NaCl
activity nearly doubled in presence of salts; activity nearly doubled in presence of salts
Pseudomonas putida
NH4Cl
activity nearly doubled in presence of salts; activity nearly doubled in presence of salts
Pseudomonas putida
Zn2+
1.9 mol of Zn2+ per mol subunit; 1.9 mol of Zn2+ per mol subunit
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38519
-
2 * 38519, sequence of cDNA
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzyl alcohol + NAD+
Pseudomonas putida
-
benzaldehyde + NADH
-
Pseudomonas putida
r
benzyl alcohol + NAD+
Pseudomonas putida
-
benzaldehyde + NADH
-
Pseudomonas putida
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
P39849
-
-
Pseudomonas putida
-
-
-
Reaction
Reaction
Commentary
Organism
an aromatic alcohol + NAD+ = an aromatic aldehyde + NADH + H+
ordered bi-bi kinetic mechanism
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chlorobenzyl alcohol + NAD+
-
389594
Pseudomonas putida
3-chlorobenzaldehyde + NADH
-
-
-
r
3-methoxybenzyl alcohol + NAD+
-
389594
Pseudomonas putida
3-methoxybenzaldehyde + NADH
-
-
-
r
3-methylbenzyl alcohol + NAD+
-
389594
Pseudomonas putida
3-methylbenzaldehyde + NADH
-
-
-
-
benzyl alcohol + NAD+
-
389594
Pseudomonas putida
benzaldehyde + NADH
-
389594
Pseudomonas putida
r
benzyl alcohol + NAD+
-
389594
Pseudomonas putida
benzaldehyde + NADH
-
389594
Pseudomonas putida
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 38519, sequence of cDNA
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.7
-
benzaldehyde + NADH
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
;
Pseudomonas putida
NADH
;
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADH
-
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Pseudomonas putida
additional information
product inhibition
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
0.065
benzaldehyde
-
Pseudomonas putida
0.145
0.155
benzyl alcohol
-
Pseudomonas putida
0.195
0.225
NAD+
-
Pseudomonas putida
0.46
1.49
NADH
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KBr
activity nearly doubled in presence of salts
Pseudomonas putida
KCl
activity nearly doubled in presence of salts
Pseudomonas putida
KCl
-
Pseudomonas putida
KNO3
activity nearly doubled in presence of salts
Pseudomonas putida
NaCl
activity nearly doubled in presence of salts
Pseudomonas putida
NH4Cl
activity nearly doubled in presence of salts
Pseudomonas putida
Zn2+
1.9 mol of Zn2+ per mol subunit
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38519
-
2 * 38519, sequence of cDNA
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
benzyl alcohol + NAD+
Pseudomonas putida
-
benzaldehyde + NADH
-
Pseudomonas putida
r
benzyl alcohol + NAD+
Pseudomonas putida
-
benzaldehyde + NADH
-
Pseudomonas putida
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-chlorobenzyl alcohol + NAD+
-
389594
Pseudomonas putida
3-chlorobenzaldehyde + NADH
-
-
-
r
3-methoxybenzyl alcohol + NAD+
-
389594
Pseudomonas putida
3-methoxybenzaldehyde + NADH
-
-
-
r
3-methylbenzyl alcohol + NAD+
-
389594
Pseudomonas putida
3-methylbenzaldehyde + NADH
-
-
-
-
benzyl alcohol + NAD+
-
389594
Pseudomonas putida
benzaldehyde + NADH
-
389594
Pseudomonas putida
r
benzyl alcohol + NAD+
-
389594
Pseudomonas putida
benzaldehyde + NADH
-
389594
Pseudomonas putida
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 38519, sequence of cDNA
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.7
-
benzaldehyde + NADH
Pseudomonas putida
Other publictions for EC 1.1.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735498
Ying
Characterization of an allylic ...
Yokenella sp. WZY002
Appl. Environ. Microbiol.
80
2399-2409
2014
-
-
1
-
-
-
3
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
5
1
2
-
1
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
3
-
-
-
1
-
-
1
-
-
-
-
-
-
-
5
1
2
-
1
-
2
-
-
-
-
-
-
-
-
-
724027
Uthoff
Purification and characterizat ...
Acinetobacter baylyi
Appl. Environ. Microbiol.
78
8743-8752
2012
-
-
1
-
-
-
2
2
-
7
2
-
-
1
-
-
1
-
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
2
-
7
2
-
-
-
-
1
-
-
-
-
16
1
1
-
2
2
1
-
-
-
-
1
1
-
2
2
724592
Nishimura
Characterization of two isozym ...
Streptomyces sp., Streptomyces sp. NL15-2K
Biosci. Biotechnol. Biochem.
75
1770-1777
2011
-
-
-
-
-
-
4
4
-
-
2
-
-
3
-
-
1
-
-
-
-
-
8
1
1
-
1
4
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
4
-
4
-
-
2
-
-
-
-
1
-
-
-
-
8
1
1
-
1
4
1
-
1
-
-
-
-
-
4
4
692723
Landete
Characterization of a benzyl a ...
Lactobacillus plantarum, Lactobacillus plantarum WCFS1, Lactobacillus plantarum WCFS1 NCIMB 8826
J. Agric. Food Chem.
56
4497-4503
2008
-
-
1
-
-
-
12
2
-
11
1
9
-
7
-
-
1
-
-
-
-
-
24
1
2
2
2
1
2
1
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
2
-
11
1
9
-
-
-
1
-
-
-
-
24
1
2
2
2
1
2
1
-
1
-
-
-
-
-
-
699815
Matsuzaki
Proteomic and metabolomic anal ...
Phanerochaete chrysosporium
J. Proteome Res.
7
2342-2350
2008
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
667287
Peng
Characterization of four Rhodo ...
Rhodococcus erythropolis, Rhodococcus erythropolis PR4
Appl. Microbiol. Biotechnol.
71
824-832
2006
-
-
1
-
-
-
-
-
-
-
-
39
-
6
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
39
-
-
-
-
-
-
-
-
45
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667256
Garcia-Pena
Correlation of biological acti ...
Paecilomyces variotii, Paecilomyces variotii CBS115145
Appl. Environ. Microbiol.
71
4280-4285
2005
-
1
-
-
-
-
-
-
-
1
-
4
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
4
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670853
Shimizu
Metabolic regulation at the tr ...
Phanerochaete chrysosporium
Proteomics
5
3919-3931
2005
1
-
-
-
-
-
-
-
1
-
1
1
-
3
-
-
1
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
657089
Millanes
-
Histochemical detection of an ...
Evernia prunastri
Plant Physiol. Biochem.
41
786-791
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654264
Overhage
Biotransformation of eugenol t ...
Cupriavidus necator, Pseudomonas putida
Appl. Environ. Microbiol.
68
4315-4321
2002
-
2
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
2
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655896
Ichinose
Molecular analysis of arylalco ...
Trametes versicolor
J. Basic Microbiol.
42
327-336
2002
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389593
Curtis
Allylic or benzylic stabilizat ...
Acinetobacter calcoaceticus, Pseudomonas putida
Biochem. Biophys. Res. Commun.
259
220-223
1999
-
-
2
-
-
-
-
4
-
-
-
2
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
14
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
389595
Inoue
Proton transfer in benzyl alco ...
Pseudomonas putida
Biochemistry
37
3305-3311
1998
-
-
-
-
2
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389596
Biegert
Purification and properties of ...
Thauera sp., Thauera sp. K172
Arch. Microbiol.
163
418-423
1995
-
-
-
-
-
-
3
3
-
-
2
2
-
7
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
2
2
-
-
-
-
-
-
1
-
6
1
-
-
-
1
2
2
-
-
-
-
-
-
-
-
389594
Shaw
Kinetic studies on benzyl alco ...
Pseudomonas putida
J. Biol. Chem.
268
10842-10850
1993
-
-
-
-
-
-
2
4
-
6
1
2
-
5
-
-
-
1
-
-
-
-
8
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
4
-
-
-
-
3
-
4
-
12
1
2
-
-
-
-
-
-
-
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288328
Chalmers
-
Purification of the benzyl alc ...
Acinetobacter calcoaceticus, Pseudomonas putida
J. Gen. Microbiol.
136
637-643
1990
-
-
-
-
-
-
-
10
-
-
2
3
-
2
-
-
1
-
-
-
1
1
9
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
10
-
-
2
3
-
-
-
1
-
-
1
1
9
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
389581
Shaw
Purification and characterisat ...
Pseudomonas putida
Eur. J. Biochem.
191
705-714
1990
-
-
-
-
-
4
6
4
-
6
2
1
-
2
-
-
1
-
-
-
1
1
16
1
1
-
2
2
1
1
-
3
1
-
-
-
-
-
3
-
-
4
-
6
1
4
-
6
2
1
-
-
-
1
-
-
1
1
16
1
1
-
2
2
1
1
-
-
-
-
-
-
-
-
288329
Harayama
Characterization of five genes ...
Pseudomonas putida
J. Bacteriol.
171
5048-5055
1989
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389583
Abril
Regulator and enzyme specifici ...
Pseudomonas putida
J. Bacteriol.
171
6782-6790
1989
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389586
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
250
743-751
1988
-
-
-
-
-
-
-
2
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2
1
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3
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1
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1
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1
1
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-
2
-
-
2
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-
2
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-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
2
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-
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389587
MacKintosh
Benzyl alcohol dehydrogenase a ...
Acinetobacter calcoaceticus
Biochem. J.
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653-661
1988
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11
12
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1
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2
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15
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7
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2
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2
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11
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12
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1
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-
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15
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7
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389590
Collins
-
Benzyl alcohol metabolism by P ...
Pseudomonas putida
Arch. Microbiol.
138
153-160
1984
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-
-
-
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1
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2
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2
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-
389591
Yamanaka
-
Identification and characteriz ...
Rhodoblastus acidophilus
Agric. Biol. Chem.
48
1161-1171
1984
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-
-
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1
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1
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1
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1
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2
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2
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2
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1
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1
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1
-
2
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-
389592
Jaaska
-
Isoenzymes of aromatic alcohol ...
Dasypyrum villosum, Elymus repens, Secale cereale, Secale montanum, Secale sylvestre, Triticale turgidocereale, Triticum monococcum subsp. aegilopoides, Triticum turgidum
Biochem. Physiol. Pflanz.
179
21-30
1984
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16
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8
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8
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1
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16
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16
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16
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8
-
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1
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389585
Inouye
Molecular cloning of TOL genes ...
Pseudomonas putida
J. Bacteriol.
145
1137-1143
1981
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-
1
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4
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2
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1
2
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389588
Poh
Evidence for isofunctional enz ...
Pseudomonas alcaligenes
J. Bacteriol.
143
59-69
1980
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1
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2
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2
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389589
Keat
The aromatic alcohol dehydroge ...
Pseudomonas putida
Biochem. J.
175
659-667
1978
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1
1
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3
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1
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1
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1
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1
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1
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2
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2
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1
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1
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3
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1
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1
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1
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1
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