BRENDA - Enzyme Database show
show all sequences of 1.1.1.88

Structural determinants of nucleotide coenzyme specificity in the distinctive dinucleotide binding fold of HMG-CoA reductase from Pseudomonas mevalonii

Friesen, J.A.; Lawrence, C.M.; Stauffacher, C.V.; Rodwell, V.W.; Biochemistry 35, 11945-11950 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21
Pseudomonas mevalonii
Engineering
Amino acid exchange
Commentary
Organism
D146A
6670-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146A/L148K
72200-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146A/L148R
83300-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146A/Q147K
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146A/T192K
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146A/T192R
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146G
1170-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146G/L148K
55600-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146G/L148R
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146G/T192K
3170-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146G/T192R
4500-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146N
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146S
no activity with NADP+ as cofactor
Pseudomonas mevalonii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
NAD+
wild-type enzyme, oxidative acylation of mevalonate
Pseudomonas mevalonii
52
-
NADP+
wild-type enzyme, oxidative acylation of mevalonate
Pseudomonas mevalonii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas mevalonii
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
very detailed kinetic studies of mutants concerning NAD+/NADP+-affinities, the best specificity improvement is achieved by the D146A/L148R-mutant
Pseudomonas mevalonii
NAD+
-
Pseudomonas mevalonii
NADH
reverse reaction
Pseudomonas mevalonii
NADP+
NAD+ is 600000fold more efficient than NADP+ in wild-type enzyme
Pseudomonas mevalonii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21
Pseudomonas mevalonii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
very detailed kinetic studies of mutants concerning NAD+/NADP+-affinities, the best specificity improvement is achieved by the D146A/L148R-mutant
Pseudomonas mevalonii
NAD+
-
Pseudomonas mevalonii
NADH
reverse reaction
Pseudomonas mevalonii
NADP+
NAD+ is 600000fold more efficient than NADP+ in wild-type enzyme
Pseudomonas mevalonii
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D146A
6670-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146A/L148K
72200-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146A/L148R
83300-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146A/Q147K
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146A/T192K
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146A/T192R
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146G
1170-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146G/L148K
55600-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146G/L148R
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146G/T192K
3170-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146G/T192R
4500-fold more specific for NADP+ than wild-type
Pseudomonas mevalonii
D146N
no activity with NADP+ as cofactor
Pseudomonas mevalonii
D146S
no activity with NADP+ as cofactor
Pseudomonas mevalonii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.21
-
NAD+
wild-type enzyme, oxidative acylation of mevalonate
Pseudomonas mevalonii
52
-
NADP+
wild-type enzyme, oxidative acylation of mevalonate
Pseudomonas mevalonii
Other publictions for EC 1.1.1.88
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723092
Ma
Optimization of a heterologous ...
Bordetella petrii, Delftia acidovorans, Pseudomonas mevalonii
Metab. Eng.
13
588-597
2011
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3
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3
3
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712189
Perchellet
Novel synthetic inhibitors of ...
Homo sapiens, Mus musculus
Int. J. Mol. Med.
24
633-643
2009
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712196
Yu
-
Expression of low-density lipo ...
Homo sapiens
Int. J. Ophthal.
9
284-285
2009
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1
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1
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712560
Martini
3-Hydroxy 3-methylglutaryl coe ...
Rattus norvegicus
J. Cell. Physiol.
220
524-530
2009
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1
1
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1
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712670
Slotta
Inhibition of 3-hydroxy-3-meth ...
Mus musculus
J. Investig. Med.
57
645-649
2009
-
1
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1
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1
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1
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687173
Azenabor
Chlamydia trachomatis Infectio ...
Homo sapiens
Int. J. Med. Sci.
4
223-231
2007
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688564
Shirai
Effect of docosahexaenoic acid ...
Mus musculus
J. Nutr. Biochem.
18
488-494
2007
1
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1
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2
1
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1
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1
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689467
Kevei
3-hydroxy-3-methylglutaryl coe ...
Medicago truncatula
Plant Cell
19
3974-3989
2007
-
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-
1
-
1
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1
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5
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2
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657303
Hedl
Inhibition of the Class II HMG ...
Pseudomonas mevalonii
Protein Sci.
13
1693-1697
2004
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6
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654225
Ayora-Talavera
Overexpression in Catharanthus ...
Mesocricetus auratus
Appl. Biochem. Biotechnol.
97
135-145
2002
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656615
Crane
-
Regulated accumulation of 3-hy ...
Solanum tuberosum
J. Plant Physiol.
159
1301-1307
2002
2
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2
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2
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287507
Tabernero
Substrate-induced closure of t ...
Pseudomonas mevalonii
Proc. Natl. Acad. Sci. USA
96
7167-7177
1999
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287508
Bochar
Aminoethylcysteine can replace ...
Pseudomonas mevalonii
Biochemistry
38
8879-8883
1999
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1
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287506
Rogers
Active form of Pseudomonas mev ...
Pseudomonas mevalonii
Biochem. Mol. Med.
61
114-120
1997
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1
3
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1
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287505
Friesen
Structural determinants of nuc ...
Pseudomonas mevalonii
Biochemistry
35
11945-11950
1996
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1
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13
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2
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13
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287504
Lawrence
Crystal structure of Pseudomon ...
Pseudomonas mevalonii
Science
268
1758-1762
1995
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287503
Sato
Replacement of serine-871 of h ...
Mesocricetus auratus
Proc. Natl. Acad. Sci. USA
90
9261-9265
1993
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287502
Darnay
Identification of the catalyti ...
Pseudomonas mevalonii
J. Biol. Chem.
267
15064-15070
1992
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15
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5
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1
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15
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2
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5
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287499
Jordan-Starck
Pseudomonas mevalonii 3-hydrox ...
Pseudomonas mevalonii
J. Biol. Chem.
264
17913-17918
1989
2
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8
1
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287500
Jordan-Starck
Role of cysteine residues in P ...
Pseudomonas mevalonii
J. Biol. Chem.
264
17919-17923
1989
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3
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4
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1
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287501
Beach
Cloning, sequencing, and overe ...
Pseudomonas mevalonii
J. Bacteriol.
171
2994 - 3001
1989
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1
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287498
Gill
Mevalonate utilization in Pseu ...
Pseudomonas mevalonii
J. Biol. Chem.
260
9393-9398
1985
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4
1
1
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1
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1
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4
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1
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1
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1
1
2
1
1
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1
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287497
Rodwell
S-3-Hydroxy-3-methylglutaryl-C ...
Pseudomonas mevalonii
Methods Enzymol.
71
480-486
1981
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287496
Bensch
Purification and properties of ...
Pseudomonas mevalonii
J. Biol. Chem.
245
3755-3762
1970
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287495
Fimognari
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Substrate-competitive inhibiti ...
Mycobacterium sp., Mycobacterium sp. s4, Pseudomonas mevalonii
Biochemistry
4
2086-2090
1965
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