Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Hydroxy-3-methylbutyrate | - |
Mycobacterium sp. | |
3-Hydroxy-3-methylbutyrate | - |
Pseudomonas mevalonii | |
3-hydroxy-3-methylcarboxylic acid | competitive inhibitor | Mycobacterium sp. | |
3-hydroxy-3-methylcarboxylic acid | competitive inhibitor | Pseudomonas mevalonii | |
3-hydroxy-3-methylglutarate | - |
Mycobacterium sp. | |
3-hydroxy-3-methylglutarate | - |
Pseudomonas mevalonii | |
3-hydroxybutyrate | - |
Mycobacterium sp. | |
3-hydroxybutyrate | - |
Pseudomonas mevalonii | |
deoxycholate | competitive inhibitor | Mycobacterium sp. | |
deoxycholate | competitive inhibitor | Pseudomonas mevalonii | |
additional information | no inhibition citrate, succinate, glutarate, 3,3-dimethylacrylate, 2,2-dimethylpropionate, acetoacetate | Mycobacterium sp. | |
additional information | no inhibition citrate, succinate, glutarate, 3,3-dimethylacrylate, 2,2-dimethylpropionate, acetoacetate | Pseudomonas mevalonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
CoA | oxidative acylation of mevalonate | Mycobacterium sp. | |
0.1 | - |
CoA | oxidative acylation of mevalonate | Pseudomonas mevalonii | |
0.35 | - |
NAD+ | oxidative acylation of mevalonate | Pseudomonas mevalonii | |
0.35 | - |
DL-mevalonate | oxidative acylation of mevalonate | Pseudomonas mevalonii | |
0.49 | - |
DL-mevalonate | oxidative acylation of mevalonate | Mycobacterium sp. | |
0.6 | - |
NAD+ | oxidative acylation of mevalonate | Mycobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mycobacterium sp. | 5829 | - |
cytosol | - |
Pseudomonas mevalonii | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | - |
S4 | - |
Mycobacterium sp. s4 | - |
S4 | - |
Pseudomonas mevalonii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.163 | - |
mevalonate acylation | Pseudomonas mevalonii |
0.17 | - |
mevalonate acylation | Mycobacterium sp. |
Storage Stability | Organism |
---|---|
-20°C stable to repeated freezing and thawing | Pseudomonas mevalonii |
-20°C unstable to repeated freezing and thawing | Mycobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-mevalonate + CoA + 2 NAD+ | - |
Mycobacterium sp. | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
? | |
(R)-mevalonate + CoA + 2 NAD+ | - |
Pseudomonas mevalonii | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
? | |
(R)-mevalonate + CoA + 2 NAD+ | - |
Mycobacterium sp. s4 | (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + NADH + H+ | - |
Pseudomonas mevalonii | mevalonate + CoA + NAD+ | - |
? | |
DL-mevalonate + CoA + NADP+ | - |
Mycobacterium sp. | 3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ | - |
r | |
DL-mevalonate + CoA + NADP+ | - |
Mycobacterium sp. s4 | 3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ | - |
r | |
additional information | oxidative acylation of mevalonate: the carboxy-, 3-methyl-, and 3-hydroxy groups are essential for substrate binding to the enzyme | Mycobacterium sp. | ? | - |
? | |
additional information | oxidative acylation of mevalonate: the carboxy-, 3-methyl-, and 3-hydroxy groups are essential for substrate binding to the enzyme | Pseudomonas mevalonii | ? | - |
? | |
additional information | oxidative acylation of mevalonate: the carboxy-, 3-methyl-, and 3-hydroxy groups are essential for substrate binding to the enzyme | Mycobacterium sp. s4 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
assay conditions | Mycobacterium sp. |
7.1 | - |
assay conditions | Pseudomonas mevalonii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mycobacterium sp. | |
NAD+ | - |
Pseudomonas mevalonii | |
NADH | reverse reaction | Pseudomonas mevalonii |