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show all sequences of 1.1.1.87

Substrate specificity analysis and inhibitor design of homoisocitrate dehydrogenase

Yamamoto, T.; Miyazaki, K.; Eguchi, T.; Bioorg. Med. Chem. 15, 1346-1355 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
(R,E)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,E)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
3-vinylmalate
-
Deinococcus radiodurans
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview
Deinococcus radiodurans
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview, no inhibition by 3-vinylmalate
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
homoisocitrate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.083
-
3-vinylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.211
-
homoisocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.29
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.291
-
isocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.41
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.44
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.47
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.51
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.58
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.59
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.6
-
ethylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.78
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
1
-
ethylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
1.33
-
isopropylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
2.1
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
2.7
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
2.8
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
3.4
-
3-vinylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
9.4
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Deinococcus radiodurans
Mg2+
-
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Deinococcus radiodurans
-
-
-
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
substrate specificity, recombinant enzyme, overview
Deinococcus radiodurans
additional information
-
substrate specificity, recombinant enzyme, overview
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
3-vinylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
3-vinylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
ethylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
ethylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
homoisocitrate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
homoisocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
isocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
isopropylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
additional information
no activity with isocitrate, isopropylmalate, and tartrate
667934
Saccharomyces cerevisiae
?
-
-
-
-
additional information
no activity with tartrate
667934
Deinococcus radiodurans
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
assay at
Deinococcus radiodurans
36
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.14
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.34
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.37
-
isopropylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.5
-
3-vinylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.61
-
ethylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.7
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.77
-
3-vinylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.78
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
2
8
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
2.1
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
3
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
4.8
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
17
-
homoisocitrate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
25
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
29
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
37
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
43
-
ethylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
43
-
isocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
46
-
homoisocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
53
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Deinococcus radiodurans
7.8
-
assay at
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Deinococcus radiodurans
NAD+
-
Saccharomyces cerevisiae
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.072
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.088
-
3-vinylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.26
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.51
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.79
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
1.4
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
3.1
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
5.2
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
15.3
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Deinococcus radiodurans
NAD+
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R,E)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,E)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,E)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,Z)-(3-carboxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Deinococcus radiodurans
(R,Z)-(3-hydroxypropylidene)malic acid
competitive
Saccharomyces cerevisiae
3-vinylmalate
-
Deinococcus radiodurans
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview
Deinococcus radiodurans
additional information
synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview, no inhibition by 3-vinylmalate
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.072
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.088
-
3-vinylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.26
-
(R,Z)-(3-carboxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.51
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.79
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
1.4
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
3.1
-
(R,E)-(3-carboxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
5.2
-
(R,E)-(3-hydroxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
15.3
-
(R,Z)-(3-hydroxypropylidene)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
homoisocitrate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.083
-
3-vinylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.211
-
homoisocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.29
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.291
-
isocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.41
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.44
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.47
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.51
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.58
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.59
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.6
-
ethylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.78
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
1
-
ethylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
1.33
-
isopropylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
2.1
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
2.7
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
2.8
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
3.4
-
3-vinylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
9.4
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Deinococcus radiodurans
Mg2+
-
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
substrate specificity, recombinant enzyme, overview
Deinococcus radiodurans
additional information
-
substrate specificity, recombinant enzyme, overview
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-allylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
(2R,3S)-3-propylmalic acid + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
3-vinylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
3-vinylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
ethylmalate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
ethylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
homoisocitrate + NAD+
-
667934
Saccharomyces cerevisiae
?
-
-
-
?
homoisocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
isocitrate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
isopropylmalate + NAD+
-
667934
Deinococcus radiodurans
?
-
-
-
?
additional information
no activity with isocitrate, isopropylmalate, and tartrate
667934
Saccharomyces cerevisiae
?
-
-
-
-
additional information
no activity with tartrate
667934
Deinococcus radiodurans
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
28
-
assay at
Deinococcus radiodurans
36
-
assay at
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.14
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.34
-
(2R,3S)-3-allylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.37
-
isopropylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.5
-
3-vinylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.61
-
ethylmalate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.7
-
(2R,3S)-3-propylmalic acid
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
0.77
-
3-vinylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
0.78
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
2
8
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
2.1
-
(2R,3S)-3-(4-hydroxybutyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
3
-
(2R,3S)-3-(4-pentenyl)malic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
4.8
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
17
-
homoisocitrate
pH 7.8, 36C, recombinant enzyme
Saccharomyces cerevisiae
25
-
(2R,3S)-3-(2-hydroxyethyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
29
-
(2R,3S)-3-(3-hydroxypropyl)malate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
37
-
(2R,3S)-3-allylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
43
-
ethylmalate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
43
-
isocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
46
-
homoisocitrate
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
53
-
(2R,3S)-3-propylmalic acid
pH 7.8, 28C, recombinant enzyme
Deinococcus radiodurans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Deinococcus radiodurans
7.8
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722303
Gabriel
Homoisocitrate dehydrogenase f ...
Candida albicans, Candida albicans ATCC 10231
FEMS Yeast Res.
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143-155
2013
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4
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690842
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Characterization of key residu ...
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692705
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RDH10 has 11-cis-retinol dehyd ...
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1
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696350
Lin
Site-directed mutagenesis as a ...
Saccharomyces cerevisiae
Biochemistry
48
7305-7312
2009
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2
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2
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2
1
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685232
Lin
Chemical mechanism of homoisoc ...
Saccharomyces cerevisiae
Biochemistry
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2
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1
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2
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1
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1
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4
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1
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1
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685539
Yamamoto
Thiahomoisocitrate: a highly p ...
Saccharomyces cerevisiae
Bioorg. Med. Chem.
16
3372-3376
2008
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1
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3
3
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1
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3
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3
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687735
Belyaeva
Kinetic analysis of human enzy ...
Homo sapiens
J. Biol. Chem.
283
20299-20308
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9
1
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3
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9
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1
9
1
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687988
Romand
Dynamic expression of the reti ...
Mus musculus
J. Comp. Neurol.
508
879-892
2008
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9
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9
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696220
Lin
Potassium is an activator of h ...
Saccharomyces cerevisiae
Biochemistry
47
10809-10815
2008
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3
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5
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1
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2
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1
1
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1
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1
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1
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3
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1
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1
1
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667934
Yamamoto
Substrate specificity analysis ...
Deinococcus radiodurans, Saccharomyces cerevisiae
Bioorg. Med. Chem.
15
1346-1355
2007
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1
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11
20
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2
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7
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1
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2
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24
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2
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20
2
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2
9
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1
2
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11
9
20
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2
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2
-
24
-
2
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20
2
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-
686287
Cammas
Expression of the murine retin ...
Mus musculus
Dev. Dyn.
236
2899-2908
2007
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686950
Sandell
RDH10 is essential for synthes ...
Mus musculus
Genes Dev.
21
1113-1124
2007
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1
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1
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669670
Szamtari
PPARgamma controls CD1d expres ...
Homo sapiens
J. Exp. Med.
203
2351-2362
2006
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-
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660907
Miyazaki
Bifunctional isocitrate-homois ...
Pyrococcus horikoshii
Biochem. Biophys. Res. Commun.
331
341-346
2005
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1
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1
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Miyazaki
Identification of a novel trif ...
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Miyazaki
Crystal structure of tetrameri ...
Thermus thermophilus
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1
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Miyazaki
Characterization of homoisocit ...
Thermus thermophilus
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4
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692332
Picozzi
Genomic organization and trans ...
Homo sapiens
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692700
Wu
Cloning and characterization o ...
Bos taurus, Homo sapiens, Mus musculus
Invest. Ophthalmol. Vis. Sci.
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3365-3372
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12
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9
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9
1
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1
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Garrad
Lysine biosynthesis in selecte ...
Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Saccharomyces cerevisiae
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1992
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7
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Lysine biosynthesis pathway an ...
Schizosaccharomyces pombe
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Gaillardin
Wild-type and mutant forms of ...
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2
2
1
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644541
Broquist
-
Homoisocitric Dehydrogenase ...
Saccharomyces cerevisiae
Methods Enzymol.
17B
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1971
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Homoisocitric dehydrogenase fr ...
Saccharomyces cerevisiae
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2
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2
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1
1
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1
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3
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1
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2
2
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644543
Strassmann
Enzymatic formation of alpha-k ...
Saccharomyces cerevisiae
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240
4357-4361
1965
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1
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2
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1
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1
1
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1
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1
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1
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1
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2
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1
1
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