Data extracted from this reference:
Cloned(Commentary)
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
Pyrococcus horikoshii
KM Value [mM]
0.0164
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
0.0183
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
Metals/Ions
Mn2+
Pyrococcus horikoshii
Natural Substrates/ Products (Substrates)
3-isopropylmalate + NAD+
Pyrococcus horikoshii
?
?
homoisocitrate + NAD+
Pyrococcus horikoshii
?
?
isocitrate + NAD+
Pyrococcus horikoshii
?
?
additional information
Pyrococcus horikoshii
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
?
Organism
Pyrococcus horikoshii
A4CYJ9
gene PH1722 or hdh
Purification (Commentary)
recombinnat His-tagged enzyme from Escherichia coli by metal affinity chromatography
Pyrococcus horikoshii
Specific Activity [micromol/min/mg]
additional information
Pyrococcus horikoshii
Substrates and Products (Substrate)
3-isopropylmalate + NAD+
660907
Pyrococcus horikoshii
?
?
homoisocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
isocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
additional information
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
660907
Pyrococcus horikoshii
?
Temperature Optimum [°C]
70
assay at
Pyrococcus horikoshii
Turnover Number [1/s]
13.7
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
14.8
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
pH Optimum
7.8
assay at
Pyrococcus horikoshii
Cofactor
NAD+
Pyrococcus horikoshii
Cloned(Commentary) (protein specific)
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
Pyrococcus horikoshii
Cofactor (protein specific)
NAD+
Pyrococcus horikoshii
KM Value [mM] (protein specific)
0.0164
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
0.0183
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
Metals/Ions (protein specific)
Mn2+
Pyrococcus horikoshii
Natural Substrates/ Products (Substrates) (protein specific)
3-isopropylmalate + NAD+
Pyrococcus horikoshii
?
?
homoisocitrate + NAD+
Pyrococcus horikoshii
?
?
isocitrate + NAD+
Pyrococcus horikoshii
?
?
additional information
Pyrococcus horikoshii
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
?
Purification (Commentary) (protein specific)
recombinnat His-tagged enzyme from Escherichia coli by metal affinity chromatography
Pyrococcus horikoshii
Specific Activity [micromol/min/mg] (protein specific)
additional information
Pyrococcus horikoshii
Substrates and Products (Substrate) (protein specific)
3-isopropylmalate + NAD+
660907
Pyrococcus horikoshii
?
?
homoisocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
isocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
additional information
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
660907
Pyrococcus horikoshii
?
Temperature Optimum [°C] (protein specific)
70
assay at
Pyrococcus horikoshii
Turnover Number [1/s] (protein specific)
13.7
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
14.8
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
pH Optimum (protein specific)
7.8
assay at
Pyrococcus horikoshii
Other publictions for EC 1.1.1.87
739958
Hsu
Evidence for an induced confor ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
584
20-27
2015
-
-
1
-
3
-
2
1
-
2
-
1
-
3
-
-
1
1
-
-
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2
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1
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1
1
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2
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1
2
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2
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1
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2
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1
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1
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-
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2
-
1
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-
1
1
-
-
-
3
3
-
-
-
722303
Gabriel
Homoisocitrate dehydrogenase f ...
Candida albicans, Candida albicans ATCC 10231
FEMS Yeast Res.
13
143-155
2013
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1
-
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-
3
4
-
2
3
2
-
8
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1
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6
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1
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4
1
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1
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1
1
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3
3
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2
3
2
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6
2
1
-
-
4
1
-
-
-
-
1
1
-
4
4
722569
Nango
Structure of Thermus thermophi ...
Thermus thermophilus
J. Biochem.
150
607-614
2011
-
-
-
1
-
-
2
1
-
1
-
1
-
5
-
-
-
-
-
-
-
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2
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1
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-
-
1
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1
1
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1
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1
1
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-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
696350
Lin
Site-directed mutagenesis as a ...
Saccharomyces cerevisiae
Biochemistry
48
7305-7312
2009
-
-
-
-
2
-
-
1
-
2
-
1
-
2
-
-
-
-
-
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1
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1
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1
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1
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2
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1
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-
-
2
1
-
-
-
-
-
-
-
-
685232
Lin
Chemical mechanism of homoisoc ...
Saccharomyces cerevisiae
Biochemistry
47
4169-4180
2008
-
-
-
-
-
-
2
5
-
1
-
-
-
2
-
-
-
1
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4
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3
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1
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1
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1
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2
1
5
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1
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4
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3
-
-
-
1
-
1
-
-
-
-
-
-
-
685539
Yamamoto
Thiahomoisocitrate: a highly p ...
Saccharomyces cerevisiae
Bioorg. Med. Chem.
16
3372-3376
2008
-
-
-
-
-
-
4
3
-
1
-
-
-
4
-
-
-
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3
-
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3
1
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3
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4
3
3
-
1
-
-
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-
-
-
-
-
-
-
3
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
687988
Romand
Dynamic expression of the reti ...
Mus musculus
J. Comp. Neurol.
508
879-892
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
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9
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-
-
-
-
-
-
-
-
-
-
-
-
-
696220
Lin
Potassium is an activator of h ...
Saccharomyces cerevisiae
Biochemistry
47
10809-10815
2008
-
-
-
-
-
-
4
3
-
5
-
1
-
2
-
-
-
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-
-
-
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3
-
1
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-
-
1
1
-
1
1
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-
-
1
-
-
-
-
4
1
3
-
5
-
1
-
-
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-
-
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-
-
3
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
667934
Yamamoto
Substrate specificity analysis ...
Deinococcus radiodurans, Saccharomyces cerevisiae
Bioorg. Med. Chem.
15
1346-1355
2007
-
-
1
-
-
-
11
20
-
2
-
-
-
7
-
-
1
-
-
-
2
-
24
-
2
-
-
20
2
-
-
2
9
-
-
-
-
1
2
-
-
-
-
11
9
20
-
2
-
-
-
-
-
1
-
-
2
-
24
-
2
-
-
20
2
-
-
-
-
-
-
-
-
-
686950
Sandell
RDH10 is essential for synthes ...
Mus musculus
Genes Dev.
21
1113-1124
2007
-
-
-
-
1
-
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-
-
-
-
1
-
1
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1
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1
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1
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1
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-
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-
-
-
660907
Miyazaki
Bifunctional isocitrate-homois ...
Pyrococcus horikoshii
Biochem. Biophys. Res. Commun.
331
341-346
2005
-
-
1
-
-
-
-
2
-
1
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4
-
3
-
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1
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1
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1
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2
1
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1
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1
1
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1
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1
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1
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4
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1
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2
1
-
-
-
-
-
-
-
-
-
660918
Miyazaki
Identification of a novel trif ...
Deinococcus radiodurans
Biochem. Biophys. Res. Commun.
336
596-602
2005
-
-
1
-
4
-
-
7
-
2
2
-
-
4
-
-
1
-
-
-
1
-
3
1
1
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7
1
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1
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1
1
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4
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7
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2
2
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1
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1
-
3
1
1
-
-
7
1
-
-
-
-
-
-
-
-
-
669109
Miyazaki
Crystal structure of tetrameri ...
Thermus thermophilus
J. Bacteriol.
187
6779-6788
2005
-
-
-
1
1
-
-
2
-
1
-
-
-
2
-
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1
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3
1
1
-
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2
1
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1
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1
1
1
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2
-
1
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1
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3
1
1
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-
2
1
-
-
-
-
-
-
-
-
-
656127
Miyazaki
Characterization of homoisocit ...
Thermus thermophilus
J. Biol. Chem.
278
1864-1871
2003
-
-
1
-
1
-
-
2
-
-
-
1
-
3
-
-
1
-
-
-
2
-
4
-
-
-
2
2
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
2
-
-
-
1
-
-
-
1
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2
-
4
-
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2
2
-
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-
-
-
-
-
-
-
-
644538
Garrad
Lysine biosynthesis in selecte ...
Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Saccharomyces cerevisiae
J. Bacteriol.
174
7379-7384
1992
-
3
1
-
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-
-
-
-
-
-
4
-
11
-
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7
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1
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7
-
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-
-
-
-
-
-
-
-
-
-
-
-
288190
Ye
Lysine biosynthesis pathway an ...
Schizosaccharomyces pombe
J. Bacteriol.
170
5968-5970
1988
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
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-
-
-
-
-
-
-
-
644540
Gaillardin
Wild-type and mutant forms of ...
Saccharomycopsis lipolytica
Eur. J. Biochem.
128
489-494
1982
-
-
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-
-
2
3
-
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3
1
1
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3
-
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1
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1
2
2
2
1
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2
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2
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2
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3
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3
1
1
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1
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1
2
2
2
1
-
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-
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-
-
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-
-
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-
644541
Broquist
-
Homoisocitric Dehydrogenase ...
Saccharomyces cerevisiae
Methods Enzymol.
17B
118-119
1971
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1
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1
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1
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1
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-
-
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-
-
644539
Rowley
Homoisocitric dehydrogenase fr ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
141
499-510
1970
2
-
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1
4
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3
1
1
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2
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1
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1
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1
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2
2
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2
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1
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1
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2
2
-
-
-
-
-
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-
-
644543
Strassmann
Enzymatic formation of alpha-k ...
Saccharomyces cerevisiae
J. Biol. Chem.
240
4357-4361
1965
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-
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1
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1
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