BRENDA - Enzyme Database show
show all sequences of 1.1.1.87

Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans

Garrad, R.C.; Bhattacharjee, J.K.; J. Bacteriol. 174, 7379-7384 (1992)

Data extracted from this reference:

Application
Application
Commentary
Organism
medicine
detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo
Aspergillus fumigatus
medicine
detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo
Candida albicans
medicine
detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo
Cryptococcus neoformans
Cloned(Commentary)
Commentary
Organism
LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli
Candida albicans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Saccharomyces cerevisiae
-
2-oxoadipate + CO2 + NADH
-
Saccharomyces cerevisiae
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Candida albicans
-
2-oxoadipate + CO2 + NADH
-
Candida albicans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Cryptococcus neoformans
-
2-oxoadipate + CO2 + NADH
-
Cryptococcus neoformans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Aspergillus fumigatus
-
2-oxoadipate + CO2 + NADH
-
Aspergillus fumigatus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
-
-
-
Candida albicans
-
-
-
Cryptococcus neoformans
-
-
-
Saccharomyces cerevisiae
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Aspergillus fumigatus
additional information
-
mutant 18804 1.4 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Candida albicans
additional information
-
several mutant with about 0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Cryptococcus neoformans
additional information
-
1.2 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Saccharomyces cerevisiae
2-oxoadipate + CO2 + NADH
-
644538
Saccharomyces cerevisiae
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Candida albicans
2-oxoadipate + CO2 + NADH
-
644538
Candida albicans
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Candida albicans
2-oxoadipate + CO2 + NADH
-
644538
Candida albicans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Cryptococcus neoformans
2-oxoadipate + CO2 + NADH
-
644538
Cryptococcus neoformans
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Cryptococcus neoformans
2-oxoadipate + CO2 + NADH
-
644538
Cryptococcus neoformans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Aspergillus fumigatus
2-oxoadipate + CO2 + NADH
-
644538
Aspergillus fumigatus
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Aspergillus fumigatus
2-oxoadipate + CO2 + NADH
-
644538
Aspergillus fumigatus
?
Application (protein specific)
Application
Commentary
Organism
medicine
detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo
Aspergillus fumigatus
medicine
detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo
Candida albicans
medicine
detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo
Cryptococcus neoformans
Cloned(Commentary) (protein specific)
Commentary
Organism
LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli
Candida albicans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Saccharomyces cerevisiae
-
2-oxoadipate + CO2 + NADH
-
Saccharomyces cerevisiae
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Candida albicans
-
2-oxoadipate + CO2 + NADH
-
Candida albicans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Cryptococcus neoformans
-
2-oxoadipate + CO2 + NADH
-
Cryptococcus neoformans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
Aspergillus fumigatus
-
2-oxoadipate + CO2 + NADH
-
Aspergillus fumigatus
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Aspergillus fumigatus
additional information
-
mutant 18804 1.4 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Candida albicans
additional information
-
several mutant with about 0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Cryptococcus neoformans
additional information
-
1.2 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Saccharomyces cerevisiae
2-oxoadipate + CO2 + NADH
-
644538
Saccharomyces cerevisiae
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Candida albicans
2-oxoadipate + CO2 + NADH
-
644538
Candida albicans
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Candida albicans
2-oxoadipate + CO2 + NADH
-
644538
Candida albicans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Cryptococcus neoformans
2-oxoadipate + CO2 + NADH
-
644538
Cryptococcus neoformans
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Cryptococcus neoformans
2-oxoadipate + CO2 + NADH
-
644538
Cryptococcus neoformans
?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Aspergillus fumigatus
2-oxoadipate + CO2 + NADH
-
644538
Aspergillus fumigatus
-
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
644538
Aspergillus fumigatus
2-oxoadipate + CO2 + NADH
-
644538
Aspergillus fumigatus
?
Other publictions for EC 1.1.1.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Candida albicans, Candida albicans ATCC 10231
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1
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3
4
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2
3
2
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8
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1
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6
2
1
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4
1
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1
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1
1
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3
3
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6
2
1
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4
1
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1
1
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4
4
722569
Nango
Structure of Thermus thermophi ...
Thermus thermophilus
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150
607-614
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1
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2
1
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1
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1
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5
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1
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1
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1
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1
1
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690842
Takahashi
Characterization of key residu ...
Homo sapiens
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419
113-122
2009
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1
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8
1
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692705
Farjo
RDH10 has 11-cis-retinol dehyd ...
Homo sapiens
Invest. Ophthalmol. Vis. Sci.
50
5089-5097
2009
1
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1
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696350
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Saccharomyces cerevisiae
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48
7305-7312
2009
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2
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1
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2
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1
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2
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1
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2
1
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685232
Lin
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Saccharomyces cerevisiae
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47
4169-4180
2008
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2
5
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1
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2
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1
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4
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3
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1
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1
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1
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2
1
5
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1
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4
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3
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1
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1
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685539
Yamamoto
Thiahomoisocitrate: a highly p ...
Saccharomyces cerevisiae
Bioorg. Med. Chem.
16
3372-3376
2008
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-
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4
3
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1
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4
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3
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3
1
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4
3
3
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1
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3
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3
1
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687735
Belyaeva
Kinetic analysis of human enzy ...
Homo sapiens
J. Biol. Chem.
283
20299-20308
2008
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1
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3
6
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1
1
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2
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1
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9
1
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2
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9
1
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687988
Romand
Dynamic expression of the reti ...
Mus musculus
J. Comp. Neurol.
508
879-892
2008
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696220
Lin
Potassium is an activator of h ...
Saccharomyces cerevisiae
Biochemistry
47
10809-10815
2008
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3
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5
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1
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2
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3
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1
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1
1
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667934
Yamamoto
Substrate specificity analysis ...
Deinococcus radiodurans, Saccharomyces cerevisiae
Bioorg. Med. Chem.
15
1346-1355
2007
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1
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11
20
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2
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7
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1
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2
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24
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2
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20
2
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2
9
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1
2
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11
9
20
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2
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1
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2
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24
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2
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20
2
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686287
Cammas
Expression of the murine retin ...
Mus musculus
Dev. Dyn.
236
2899-2908
2007
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686950
Sandell
RDH10 is essential for synthes ...
Mus musculus
Genes Dev.
21
1113-1124
2007
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669670
Szamtari
PPARgamma controls CD1d expres ...
Homo sapiens
J. Exp. Med.
203
2351-2362
2006
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660907
Miyazaki
Bifunctional isocitrate-homois ...
Pyrococcus horikoshii
Biochem. Biophys. Res. Commun.
331
341-346
2005
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1
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660918
Miyazaki
Identification of a novel trif ...
Deinococcus radiodurans
Biochem. Biophys. Res. Commun.
336
596-602
2005
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1
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4
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7
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2
2
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1
1
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1
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1
1
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7
1
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669109
Miyazaki
Crystal structure of tetrameri ...
Thermus thermophilus
J. Bacteriol.
187
6779-6788
2005
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1
1
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1
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1
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656127
Miyazaki
Characterization of homoisocit ...
Thermus thermophilus
J. Biol. Chem.
278
1864-1871
2003
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1
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1
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1
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1
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