BRENDA - Enzyme Database show
show all sequences of 1.1.1.86

Molecular annotation of ketol-acid reductoisomerases from Streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity

Verdel-Aranda, K.; Lopez-Cortina, S.T.; Hodgson, D.A.; Barona-Gomez, F.; Microb. Biotechnol. 8, 239-252 (2015)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.28
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.29
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.3
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.8
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
1.2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
1.2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
1.6
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
1.8
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
2.3
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
2.5
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
2.6
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
3
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
4
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
4
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
5
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
5
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
6.7
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
8
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
8
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
8.6
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
8.6
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
9
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
9.3
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
10
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
10
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
12
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
12
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
13
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
20
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
20
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
20
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
25
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
30
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces avermitilis
30
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces griseus
35
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces griseus
35
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
40
-
2-Methylacetoacetate
pH 8.0 25°C
Corynebacterium glutamicum
40
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces ambofaciens
40
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces pristinaespiralis
40
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
40
-
Hydroxypyruvate
pH 8.0 25°C
Corynebacterium glutamicum
40
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces coelicolor
45
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
45
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
50
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
50
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
50
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces viridifaciens
50
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
50
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
50
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
50
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces ambofaciens
50
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces lividans
50
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces viridifaciens
60
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
60
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces avermitilis
60
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
67
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
71
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
75
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
700
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
950
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
1010
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
1050
-
pyruvate
pH 8.0 25°C
Streptomyces ambofaciens
1060
-
pyruvate
pH 8.0 25°C
Streptomyces griseus
1070
-
pyruvate
pH 8.0 25°C
Corynebacterium glutamicum
1080
-
pyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
1120
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
1140
-
pyruvate
pH 8.0 25°C
Streptomyces viridifaciens
1200
-
pyruvate
pH 8.0 25°C
Streptomyces avermitilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
-
-
-
Escherichia coli
-
-
-
Streptomyces ambofaciens
A0A0K2AZ61
-
-
Streptomyces ambofaciens ATCC 23877
A0A0K2AZ61
-
-
Streptomyces avermitilis
Q59818
-
-
Streptomyces avermitilis DSM 46492
Q59818
-
-
Streptomyces coelicolor
Q9FBT8
-
-
Streptomyces coelicolor
Q9Z565
-
-
Streptomyces coelicolor ATCC BAA-471
Q9Z565
-
-
Streptomyces coelicolor BAA-471
Q9FBT8
-
-
Streptomyces griseus
-
-
-
Streptomyces lividans
D6EC76
-
-
Streptomyces lividans
D6ERQ9
-
-
Streptomyces lividans TK24
D6EC76
-
-
Streptomyces lividans TK24
D6ERQ9
-
-
Streptomyces pristinaespiralis
-
-
-
Streptomyces viridifaciens
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-2-ethyl-butanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-2-methyl-butanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces griseus
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces viridifaciens
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces coelicolor
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces lividans
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces ambofaciens
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces avermitilis
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces lividans TK24
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Corynebacterium glutamicum
3-hydroxy-2-methylbutanoate+ NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-3-ethyl-butanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-3-methyl-butanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxypropanoate+ NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Corynebacterium glutamicum
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces griseus
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces viridifaciens
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces coelicolor
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces lividans
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces ambofaciens
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces avermitilis
? + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Corynebacterium glutamicum
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces griseus
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces viridifaciens
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces coelicolor
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces lividans
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces ambofaciens
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces avermitilis
2-hydroxypropanoate + NADP+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.12
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.18
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.18
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.24
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.24
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.28
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.3
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.3
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.39
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.4
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.4
-
2-Methylacetoacetate
pH 8.0 25°C
Corynebacterium glutamicum
0.4
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.42
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.44
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.5
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.5
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.5
-
Hydroxypyruvate
pH 8.0 25°C
Corynebacterium glutamicum
0.5
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.6
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
0.63
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.67
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.7
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.7
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces lividans
0.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.8
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.9
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.9
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.9
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.9
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
1
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
1
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
1
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces ambofaciens
1
-
pyruvate
pH 8.0 25°C
Streptomyces griseus
1.05
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces griseus
1.1
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
1.2
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces ambofaciens
1.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
1.3
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
1.3
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
1.4
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
1.5
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces griseus
1.8
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces avermitilis
1.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
2
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces pristinaespiralis
2
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces viridifaciens
2
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces avermitilis
2
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
2
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces viridifaciens
2.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
2.5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
2.8
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
2.8
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
3
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
3
-
pyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
3.1
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
3.2
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
3.2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
3.5
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
3.6
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
3.6
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
3.6
-
pyruvate
pH 8.0 25°C
Streptomyces avermitilis
3.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
5.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
5.6
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
5.7
-
pyruvate
pH 8.0 25°C
Streptomyces viridifaciens
6.3
-
pyruvate
pH 8.0 25°C
Streptomyces ambofaciens
6.7
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
8.6
-
pyruvate
pH 8.0 25°C
Corynebacterium glutamicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.26
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.28
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.29
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.3
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
0.8
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
1.2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
1.2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
1.6
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
1.8
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
2.3
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
2.5
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
2.6
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
3
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
4
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
4
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
5
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
5
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
6.7
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
8
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
8
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
8.6
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
8.6
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
9
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
9.3
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
10
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
10
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
12
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
12
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
13
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
20
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
20
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
20
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
25
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
30
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces avermitilis
30
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces griseus
35
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces griseus
35
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
40
-
2-Methylacetoacetate
pH 8.0 25°C
Corynebacterium glutamicum
40
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces ambofaciens
40
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces pristinaespiralis
40
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
40
-
Hydroxypyruvate
pH 8.0 25°C
Corynebacterium glutamicum
40
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces coelicolor
45
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
45
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
50
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
50
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
50
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces viridifaciens
50
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
50
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
50
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
50
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces ambofaciens
50
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces lividans
50
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces viridifaciens
60
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
60
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces avermitilis
60
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
67
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
71
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
75
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
700
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
950
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
1010
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
1050
-
pyruvate
pH 8.0 25°C
Streptomyces ambofaciens
1060
-
pyruvate
pH 8.0 25°C
Streptomyces griseus
1070
-
pyruvate
pH 8.0 25°C
Corynebacterium glutamicum
1080
-
pyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
1120
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
1140
-
pyruvate
pH 8.0 25°C
Streptomyces viridifaciens
1200
-
pyruvate
pH 8.0 25°C
Streptomyces avermitilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-2-ethyl-butanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-ethyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxy-2-ethylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-2-methyl-butanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces griseus
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces viridifaciens
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces coelicolor
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces lividans
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces ambofaciens
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces avermitilis
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces lividans TK24
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
3-hydroxy-2-methylbutanoate + NADP+
-
-
-
?
2-methylacetoacetate + NADPH + H+
-
738989
Corynebacterium glutamicum
3-hydroxy-2-methylbutanoate+ NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-3-ethyl-butanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-ethyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxy-3-ethylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Escherichia coli
2,3-dihydroxy-3-methyl-butanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
3-hydroxy-3-methyl-2-oxobutanoate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxy-3-methylbutanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces griseus
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces viridifaciens
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces coelicolor
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces lividans
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces ambofaciens
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces avermitilis
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces ambofaciens ATCC 23877
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces coelicolor ATCC BAA-471
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces lividans TK24
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces coelicolor BAA-471
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Streptomyces avermitilis DSM 46492
2,3-dihydroxypropanoate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
738989
Corynebacterium glutamicum
2,3-dihydroxypropanoate+ NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Corynebacterium glutamicum
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces griseus
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces viridifaciens
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces coelicolor
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces lividans
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces ambofaciens
? + NADP+
-
-
-
?
pyrroline-5-carboxylate + NADPH + H+
-
738989
Streptomyces avermitilis
? + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Corynebacterium glutamicum
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces griseus
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces viridifaciens
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces pristinaespiralis
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces coelicolor
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces lividans
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces ambofaciens
2-hydroxypropanoate + NADP+
-
-
-
?
pyruvate + NADPH + H+
-
738989
Streptomyces avermitilis
2-hydroxypropanoate + NADP+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.12
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.18
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.18
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.24
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.24
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.28
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.3
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.3
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.39
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.4
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.4
-
2-Methylacetoacetate
pH 8.0 25°C
Corynebacterium glutamicum
0.4
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.42
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.44
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.5
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.5
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.5
-
Hydroxypyruvate
pH 8.0 25°C
Corynebacterium glutamicum
0.5
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.6
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
0.63
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.67
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.7
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.7
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces lividans
0.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.8
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.9
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.9
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.9
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.9
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
1
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
1
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
1
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces ambofaciens
1
-
pyruvate
pH 8.0 25°C
Streptomyces griseus
1.05
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces griseus
1.1
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
1.2
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces ambofaciens
1.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
1.3
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
1.3
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
1.4
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
1.5
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces griseus
1.8
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces avermitilis
1.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
2
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces pristinaespiralis
2
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces viridifaciens
2
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces avermitilis
2
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
2
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces viridifaciens
2.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
2.5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
2.8
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
2.8
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
3
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
3
-
pyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
3.1
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
3.2
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
3.2
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
3.5
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
3.6
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
3.6
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
3.6
-
pyruvate
pH 8.0 25°C
Streptomyces avermitilis
3.8
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
5.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
5.6
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
5.7
-
pyruvate
pH 8.0 25°C
Streptomyces viridifaciens
6.3
-
pyruvate
pH 8.0 25°C
Streptomyces ambofaciens
6.7
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
8.6
-
pyruvate
pH 8.0 25°C
Corynebacterium glutamicum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.001
-
pyruvate
pH 8.0 25°C
Streptomyces griseus
0.0024
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces coelicolor
0.0024
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces griseus
0.0028
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.003
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces coelicolor
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces avermitilis
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.0037
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces lividans
0.0038
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces viridifaciens
0.005
-
pyruvate
pH 8.0 25°C
Streptomyces viridifaciens
0.0056
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces avermitilis
0.006
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces ambofaciens
0.006
-
pyruvate
pH 8.0 25°C
Streptomyces ambofaciens
0.006
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
0.0061
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces lividans
0.008
-
pyruvate
pH 8.0 25°C
Corynebacterium glutamicum
0.008
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.01
-
2-Methylacetoacetate
pH 8.0 25°C
Corynebacterium glutamicum
0.01
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
0.01
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.01
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.012
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.012
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.015
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.018
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.02
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.02
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
0.02
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.02
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces ambofaciens
0.028
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.028
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.03
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.03
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces ambofaciens
0.03
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces griseus
0.03
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.03
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.032
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.036
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.04
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces viridifaciens
0.04
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C; pH 8.0 25°C
Streptomyces coelicolor
0.04
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces griseus
0.04
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces lividans
0.045
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.045
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.046
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C; pH 8.0 25°C
Streptomyces lividans
0.047
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.048
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.05
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.05
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.05
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces avermitilis
0.05
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces viridifaciens
0.06
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces avermitilis
0.06
-
Hydroxypyruvate
pH 8.0 25°C
Corynebacterium glutamicum
0.06
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.153
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.176
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.194
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.3
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.315
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.35
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.391
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.4
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.4
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.52
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.53
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.583
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.687
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.7
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.733
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.85
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
1.11
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
7.14
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
11.67
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
11.92
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
13.1
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.001
-
pyruvate
pH 8.0 25°C
Streptomyces griseus
0.0024
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces coelicolor
0.0024
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces griseus
0.0028
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.003
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces coelicolor
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces avermitilis
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
0.003
-
pyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.0037
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces lividans
0.0038
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces viridifaciens
0.005
-
pyruvate
pH 8.0 25°C
Streptomyces viridifaciens
0.0056
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces avermitilis
0.006
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces ambofaciens
0.006
-
pyruvate
pH 8.0 25°C
Streptomyces ambofaciens
0.006
-
pyruvate
pH 8.0 25°C
Streptomyces lividans
0.0061
-
pyrroline-5-carboxylate
pH 8.0 25°C
Streptomyces lividans
0.008
-
pyruvate
pH 8.0 25°C
Corynebacterium glutamicum
0.008
-
pyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.01
-
2-Methylacetoacetate
pH 8.0 25°C
Corynebacterium glutamicum
0.01
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
0.01
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.01
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.012
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.012
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.015
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces lividans
0.018
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.02
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.02
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces coelicolor
0.02
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.02
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces ambofaciens
0.028
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.028
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.03
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.03
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces ambofaciens
0.03
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces griseus
0.03
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.03
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.032
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.036
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.04
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces viridifaciens
0.04
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.04
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces griseus
0.04
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces lividans
0.045
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.045
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.046
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.047
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.048
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.05
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.05
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.05
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces avermitilis
0.05
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces viridifaciens
0.06
-
2-Methylacetoacetate
pH 8.0 25°C
Streptomyces avermitilis
0.06
-
Hydroxypyruvate
pH 8.0 25°C
Corynebacterium glutamicum
0.06
-
Hydroxypyruvate
pH 8.0 25°C
Streptomyces coelicolor
0.153
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.176
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.194
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.2
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.3
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.315
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces ambofaciens
0.35
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
0.391
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.4
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Corynebacterium glutamicum
0.4
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.5
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
0.52
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces avermitilis
0.53
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces pristinaespiralis
0.583
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.687
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.7
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces lividans
0.733
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces coelicolor
0.85
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Streptomyces griseus
1.11
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Streptomyces viridifaciens
7.14
-
2-Hydroxy-2-methyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
11.67
-
2-hydroxy-2-ethyl-3-oxobutanoate
pH 8.0 25°C
Escherichia coli
11.92
-
3-Hydroxy-3-methyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
13.1
-
3-hydroxy-3-ethyl-2-oxobutanoate
pH 8.0 25°C
Escherichia coli
Other publictions for EC 1.1.1.86
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738067
Tadrowski
Metal ions play an essential c ...
Escherichia coli, Oryza sativa
Chemistry
22
7427-7436
2016
-
-
-
-
-
-
-
-
-
2
-
2
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
4
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
4
2
-
-
-
-
-
-
-
4
4
738219
Lv
Crystal structure of Mycobacte ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
FEBS J.
283
1184-1196
2016
-
-
1
1
-
-
1
2
-
1
1
4
-
8
-
-
1
-
-
-
-
-
6
1
-
-
-
2
-
-
-
1
1
-
-
-
-
1
1
1
-
-
-
1
1
2
-
1
1
4
-
-
-
1
-
-
-
-
6
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
731279
Cahn
Cofactor specificity motifs an ...
Alicyclobacillus acidocaldarius subsp. acidocaldarius, Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM446, Azotobacter vinelandii, Azotobacter vinelandii ATCC BAA-1303, Ignisphaera aggregans, Ignisphaera aggregans DSM 17230, Ignisphaera aggregans DSM17230, uncultured archaeon
Biochem. J.
468
475-484
2015
-
-
-
4
-
-
-
-
-
4
-
16
-
11
-
-
-
-
-
-
-
-
16
-
-
1
1
-
-
-
-
8
-
-
-
-
-
-
8
4
-
-
-
-
-
-
-
4
-
16
-
-
-
-
-
-
-
-
16
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
737785
Reisse
Identification and optimizatio ...
Meiothermus ruber, Meiothermus ruber DSM 1279
Biochimie
108
76-84
2015
-
-
1
-
1
-
-
5
-
-
-
-
-
3
-
-
1
-
-
-
-
-
4
1
1
-
1
5
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
5
-
-
-
-
-
-
-
1
-
-
-
-
4
1
1
-
1
5
1
-
-
-
-
-
-
-
5
5
738989
Verdel-Aranda
Molecular annotation of ketol- ...
Corynebacterium glutamicum, Escherichia coli, Streptomyces ambofaciens, Streptomyces ambofaciens ATCC 23877, Streptomyces avermitilis, Streptomyces avermitilis DSM 46492, Streptomyces coelicolor, Streptomyces coelicolor ATCC BAA-471, Streptomyces coelicolor BAA-471, Streptomyces griseus, Streptomyces lividans, Streptomyces lividans TK24, Streptomyces pristinaespiralis, Streptomyces viridifaciens
Microb. Biotechnol.
8
239-252
2015
-
-
-
-
-
-
-
72
-
-
-
-
-
22
-
-
-
-
-
-
-
-
114
-
-
-
-
72
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
72
-
-
-
-
-
-
-
-
-
-
-
-
114
-
-
-
-
72
-
-
-
-
-
-
-
-
79
81
735831
Oh
Production of 2-butanol from c ...
Klebsiella pneumoniae subsp. pneumoniae, Klebsiella pneumoniae subsp. pneumoniae ATCC 700721
Biotechnol. Lett.
36
57-62
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
739528
Brinkmann-Chen
General approach to reversing ...
Alicyclobacillus acidocaldarius subsp. acidocaldarius, Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446, Methanococcus aeolicus, Methanococcus aeolicus DSM 17508
Proc. Natl. Acad. Sci. USA
110
10946-10951
2013
-
-
-
-
3
-
-
9
-
-
-
-
-
5
-
-
-
-
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1
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639178
Mrachko
The pH dependence of the kinet ...
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4
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639179
Dumas
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Isolation and kinetic properti ...
Spinacia oleracea
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1992
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1
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12
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1
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3
12
4
1
1
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1
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1
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3
1
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1
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639177
Aulabaugh
Oxalyl hydroxamates as reactio ...
Escherichia coli
Biochemistry
29
2824-2830
1990
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3
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1
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639175
Chunduru
Mechanism of ketol acid reduct ...
Escherichia coli
Biochemistry
28
486-493
1989
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5
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3
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639176
Dumas
Purification and characterizat ...
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1
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1
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1
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1
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3
1
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2
2
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639174
Hofler
The subunit structure of alpha ...
Salmonella enterica subsp. enterica serovar Typhimurium
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877-882
1975
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1
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3
1
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33969
Ryan
Subcellular localization of is ...
Saccharomyces cerevisiae
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631-637
1974
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1
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3
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639172
Shematek
A kinetic study of alpha-aceto ...
Salmonella enterica subsp. enterica serovar Typhimurium
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158
132-138
1973
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639169
Arfin
Purification and properties of ...
Salmonella enterica subsp. enterica serovar Typhimurium
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1969
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9
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7
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1
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9
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Kiritani
-
The reductoisomerase of Neuros ...
Neurospora crassa
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1966
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1
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1
2
6
1
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2
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2
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3
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1
1
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1
1
5
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1
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1
2
6
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639170
Satyanarayana
Biosynthesis of valine and iso ...
Vigna radiata var. radiata
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110
380-388
1965
1
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