Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
(2R,3S)-3-isopropylmalate | kcat/Km: 88000/Msec | Methanocaldococcus jannaschii | |
0.41 | - |
D-malate | kcat/Km: 14000/Msec | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified by heat treatment followed by nickel affinity chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Methanocaldococcus jannaschii | 2-ketoisocaproate + NADH + H+ + CO2 | - |
? | |
beta-methylmalate + NAD+ | enzyme catalyzes the final step in the pyruvate pathway to 2-oxobutyrate | Methanocaldococcus jannaschii | 2-oxobutyrate + NADH + CO2 | - |
? | |
D-malate + NAD+ | - |
Methanocaldococcus jannaschii | pyruvate + NADH + CO2 | - |
? | |
additional information | enzyme does not catalyze the oxidation of L-malate, L-tartrate, D-tartrate, DL-isocitrate or DL-lactate | Methanocaldococcus jannaschii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Methanocaldococcus jannaschii |
isopropylmalate dehydrogenase | - |
Methanocaldococcus jannaschii |