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Literature summary for 1.1.1.85 extracted from

  • Drevland, R.M.; Waheed, A.; Graham, D.E.
    Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii (2007), J. Bacteriol., 189, 4391-4400.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
(2R,3S)-3-isopropylmalate kcat/Km: 88000/Msec Methanocaldococcus jannaschii
0.41
-
D-malate kcat/Km: 14000/Msec Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
SDS-PAGE Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified by heat treatment followed by nickel affinity chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Methanocaldococcus jannaschii 2-ketoisocaproate + NADH + H+ + CO2
-
?
beta-methylmalate + NAD+ enzyme catalyzes the final step in the pyruvate pathway to 2-oxobutyrate Methanocaldococcus jannaschii 2-oxobutyrate + NADH + CO2
-
?
D-malate + NAD+
-
Methanocaldococcus jannaschii pyruvate + NADH + CO2
-
?
additional information enzyme does not catalyze the oxidation of L-malate, L-tartrate, D-tartrate, DL-isocitrate or DL-lactate Methanocaldococcus jannaschii ?
-
?

Synonyms

Synonyms Comment Organism
IPMDH
-
Methanocaldococcus jannaschii
isopropylmalate dehydrogenase
-
Methanocaldococcus jannaschii