Crystallization (Comment) | Organism |
---|---|
study of the mutant enzymes G240A and L246E/V249M by X-ray crystallography | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
G240A | decreased thermostability | Thermus thermophilus |
L246E/V249M | decreased thermostability | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
87 | - |
the extended hydrogen bonds within the beta-sheet are the major reason for the decreased thermostability of G240A | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus |