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Literature summary for 1.1.1.85 extracted from

  • Akanuma, S.; Qu, C.; Yamagishi, A.; Tanaka, N.; Oshima, T.
    Effect of polar side chains at position 172 on thermal stability of 3-isopropylmalate dehydrogenase from Thermus thermophilus (1997), FEBS Lett., 410, 141-144.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
A172D enhanced thermostability compared to wild-type enzyme Thermus thermophilus
A172E enhanced thermostability compared to wild-type enzyme Thermus thermophilus
A172N enhanced thermostability compared to wild-type enzyme Thermus thermophilus
A172Q enhanced thermostability compared to wild-type enzyme Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0169
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172D Thermus thermophilus
0.0169
-
(2R,3S)-3-isopropylmalate mutant enzyme A172D, at 55°C, pH not specified in the publication Thermus thermophilus
0.0221
-
(2R,3S)-3-isopropylmalate 55°C, wild-type enzyme Thermus thermophilus
0.0221
-
(2R,3S)-3-isopropylmalate wild type enzyme, at 55°C, pH not specified in the publication Thermus thermophilus
0.0234
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172N Thermus thermophilus
0.0234
-
(2R,3S)-3-isopropylmalate mutant enzyme A172N, at 55°C, pH not specified in the publication Thermus thermophilus
0.0257
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172E Thermus thermophilus
0.0257
-
(2R,3S)-3-isopropylmalate mutant enzyme A172E, at 55°C, pH not specified in the publication Thermus thermophilus
0.0267
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172Q Thermus thermophilus
0.0267
-
(2R,3S)-3-isopropylmalate mutant enzyme A172Q, at 55°C, pH not specified in the publication Thermus thermophilus
0.076
-
NAD+ 55°C, mutant enzyme A172D Thermus thermophilus
0.094
-
NAD+ 55°C, mutant enzyme A172Q Thermus thermophilus
0.1
-
NAD+ 55°C, mutant enzyme A172E Thermus thermophilus
0.105
-
NAD+ 55°C, mutant enzyme A172N Thermus thermophilus
0.116
-
NAD+ 55°C, wild-type enzyme Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
?
(2R,3S)-3-isopropylmalate + NAD+
-
Thermus thermophilus (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
100
-
NAD+ 55°C, mutant enzyme A172D Thermus thermophilus
100
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172D Thermus thermophilus
107
-
NAD+ 55°C, wild-type enzyme Thermus thermophilus
107
-
(2R,3S)-3-isopropylmalate 55°C, wild-type enzyme Thermus thermophilus
111
-
NAD+ 55°C, mutant enzyme A172Q Thermus thermophilus
111
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172Q Thermus thermophilus
117
-
NAD+ 55°C, mutant enzyme A172E and A172N Thermus thermophilus
117
-
(2R,3S)-3-isopropylmalate 55°C, mutant enzyme A172E and A172N Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Thermus thermophilus