BRENDA - Enzyme Database show
show all sequences of 1.1.1.85

beta-Isopropylmalate dehydrogenase (Salmonella typhimurium)

Parsons, S.J.; Burns, R.O.; Methods Enzymol. 17A, 793-799 (1970)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
dilute solutions, 1 mg/ml or less, precipitate upon thawing
Salmonella enterica subsp. enterica serovar Typhimurium
slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ba2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Ca2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Co2+
above 0.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetamide
50 mM, slight inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetate
50 mM, slight inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
K+
above 1 M, slight inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
Li+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
above 0.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
N-ethylmaleimide
-
Salmonella enterica subsp. enterica serovar Typhimurium
NEM
5 mM, 50% inhibition, not reversed by 2-mercaptoethanol
Salmonella enterica subsp. enterica serovar Typhimurium
p-hydroxymercuribenzoate
pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol
Salmonella enterica subsp. enterica serovar Typhimurium
PCMB
-
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
D-3-isopropylmalate
pH 9.0
Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
NAD+
pH 9.0
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
K+
monovalent cation required, maximal activity at 0.05 M
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
NH4+
monovalent cations required, NH4+ can partially replace K+
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl
Salmonella enterica subsp. enterica serovar Typhimurium
70000
-
equilibrium sedimentation
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2R,3S)-3-isopropylmalate + NAD+
Salmonella enterica subsp. enterica serovar Typhimurium
penultimate step in leucine biosynthesis
2-oxoisocaproate + NADH + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
strain leu A-124
-
Purification (Commentary)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
54
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability
Storage Stability
Organism
-20°C, pH 5.5-7.5, fully active
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-3-isopropylmalate + NAD+
-
639148
Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisocaproate + NADH + CO2
-
-
-
?
(2R,3S)-3-isopropylmalate + NAD+
penultimate step in leucine biosynthesis
639148
Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisocaproate + NADH + CO2
-
-
-
?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
-
639148
Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisohexanoate + thionicotinamideNADH + CO2
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
7.5
stable if stored concentrated
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Salmonella enterica subsp. enterica serovar Typhimurium
General Stability (protein specific)
General Stability
Organism
dilute solutions, 1 mg/ml or less, precipitate upon thawing
Salmonella enterica subsp. enterica serovar Typhimurium
slow inactivation occurs in presence of relatively high concentrations of EDTA, 0.01 M
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ba2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Ca2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Co2+
above 0.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetamide
50 mM, slight inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
iodoacetate
50 mM, slight inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
K+
above 1 M, slight inhibition
Salmonella enterica subsp. enterica serovar Typhimurium
Li+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
above 0.5 mM
Salmonella enterica subsp. enterica serovar Typhimurium
N-ethylmaleimide
-
Salmonella enterica subsp. enterica serovar Typhimurium
NEM
5 mM, 50% inhibition, not reversed by 2-mercaptoethanol
Salmonella enterica subsp. enterica serovar Typhimurium
p-hydroxymercuribenzoate
pH 9.0, 2 mM, complete inhibition, reversed by 2-mercaptoethanol
Salmonella enterica subsp. enterica serovar Typhimurium
PCMB
-
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.019
-
D-3-isopropylmalate
pH 9.0
Salmonella enterica subsp. enterica serovar Typhimurium
0.1
-
NAD+
pH 9.0
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
K+
monovalent cation required, maximal activity at 0.05 M
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
divalent cation required, at 0.5 mM Mn2+ causes optimal stimulation followed by Mg2+ and Co2+
Salmonella enterica subsp. enterica serovar Typhimurium
NH4+
monovalent cations required, NH4+ can partially replace K+
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl
Salmonella enterica subsp. enterica serovar Typhimurium
70000
-
equilibrium sedimentation
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2R,3S)-3-isopropylmalate + NAD+
Salmonella enterica subsp. enterica serovar Typhimurium
penultimate step in leucine biosynthesis
2-oxoisocaproate + NADH + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
54
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, pH 5.5-7.5, fully active
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-3-isopropylmalate + NAD+
-
639148
Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisocaproate + NADH + CO2
-
-
-
?
(2R,3S)-3-isopropylmalate + NAD+
penultimate step in leucine biosynthesis
639148
Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisocaproate + NADH + CO2
-
-
-
?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
-
639148
Salmonella enterica subsp. enterica serovar Typhimurium
2-oxoisohexanoate + thionicotinamideNADH + CO2
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, sedimentation equilibrium centrifugation after treatment with mercaptoethanol and 6 M guanidine-HCl
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
9.5
-
Salmonella enterica subsp. enterica serovar Typhimurium
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
7.5
stable if stored concentrated
Salmonella enterica subsp. enterica serovar Typhimurium
Other publictions for EC 1.1.1.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737729
Graczer
Dual role of the active site r ...
Thermus thermophilus
Biochemistry
55
560-574
2016
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7
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16
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1
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1
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7
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738191
Hamajima
Pressure adaptation of 3-isopr ...
Shewanella benthica, Shewanella benthica DB21MT-2, Shewanella oneidensis
Extremophiles
20
177-186
2016
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2
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6
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16
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3
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5
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6
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16
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2
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3
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16
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8
8
738195
Takahashi
Characterization of two ?-deca ...
Sulfolobus acidocaldarius, Sulfolobus acidocaldarius MW001
Extremophiles
20
843-853
2016
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1
1
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3
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1
1
2
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5
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1
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1
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6
1
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2
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2
2
738685
Lee
Structure and mechanism of iso ...
Arabidopsis thaliana
J. Biol. Chem.
291
13421-13430
2016
-
-
1
1
18
-
-
12
-
-
-
1
-
2
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1
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1
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12
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1
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1
1
1
18
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12
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1
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1
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1
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12
-
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12
12
738239
Graczer
Glutamate 270 plays an essenti ...
Thermus thermophilus
FEBS Lett.
589
240-245
2015
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-
-
1
1
-
-
4
-
3
-
1
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2
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2
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4
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1
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2
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739436
Sharma
Deciphering the dynamics of no ...
Thermus thermophilus
PLoS ONE
10
e0144294
2015
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-
-
1
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1
-
3
-
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1
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737867
Hamajima
Pressure effects on the chimer ...
Shewanella benthica, Shewanella oneidensis
Biosci. Biotechnol. Biochem.
78
469-471
2014
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-
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2
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4
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738212
Pallo
Structural and energetic basis ...
Thermus thermophilus
FEBS J.
281
5063-5076
2014
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1
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1
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724976
Graczer
Transient kinetic studies reve ...
Thermus thermophilus
FEBS J.
280
1764-1772
2013
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2
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2
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2
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739458
He
Integrated proteomics and meta ...
Arabidopsis thaliana
PLoS ONE
8
e57118
2013
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3
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3
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723829
Nagae
High-pressure-induced water pe ...
Shewanella oneidensis
Acta Crystallogr. Sect. D
68
300-309
2012
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1
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723863
Nagae
Structural analysis of 3-isopr ...
Shewanella benthica, Shewanella benthica DB21 MT-2, Shewanella oneidensis
Acta Crystallogr. Sect. F
68
265-268
2012
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2
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6
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725006
Graczer
Essential role of the metal-io ...
Thermus thermophilus
FEBS Lett.
585
3297-3302
2011
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725437
He
Structural and functional evol ...
Arabidopsis thaliana
J. Biol. Chem.
286
28794-28801
2011
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1
3
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12
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6
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6
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12
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Functional characterization of ...
Arabidopsis thaliana
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Crystallization and preliminar ...
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1
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711444
Nango
Crystal structure of 3-isoprop ...
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711511
Kasahara
Piezo-adapted (2R,3S)-3-isopro ...
Shewanella benthica, Shewanella oneidensis
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684587
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Enzymic approach to eurytherma ...
Alvinella pompejana
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701046
Sasaki
Random mutagenesis improves th ...
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2008
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Graczer
Rates of unfolding, rather tha ...
Thermus thermophilus
Biochemistry
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2007
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Ohkuri
The effects of mutations at po ...
Bacillus subtilis
J. Biochem.
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2007
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8
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1
1
1
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1
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Watanabe
The effects of multiple ancest ...
Thermus thermophilus
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2006
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1
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1
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1
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1
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669872
Watanabe
Designing thermostable protein ...
Thermus thermophilus
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2006
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1
1
1
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1
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1
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670847
Kalinina
Amino acid residues that deter ...
Acidithiobacillus ferrooxidans, Agrobacterium tumefaciens, Arthrospira platensis, Aspergillus niger, Azotobacter vinelandii, Bacteroides fragilis, Brassica napus, Candida boidinii, Candida maltosa, Clostridium pasteurianum, Cyberlindnera jadinii, Eremothecium gossypii, Escherichia coli, Leptospira interrogans, Mycobacterium bovis, Ogataea angusta, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Scheffersomyces stipitis, Thermus aquaticus, Thermus thermophilus, Zymoseptoria tritici
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Singh
The High-resolution Structure ...
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2005
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1
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668015
Nozawa
Cloning of cDNAs encoding isop ...
Arabidopsis thaliana
Biosci. Biotechnol. Biochem.
69
806-810
2005
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1
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1
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6
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1
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1
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6
-
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2
-
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-
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-
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670886
Lunzer
The biochemical architecture o ...
Escherichia coli
Science
310
499-501
2005
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1
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1
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1
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1
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1
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1
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1
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1
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1
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671017
Turakainen
Cloning, sequencing and applic ...
Candida humilis, Candida humilis C175/CBS
Yeast
22
805-812
2005
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1
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7
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1
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-
-
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-
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-
657230
Ohkuri
Increased thermal stability ag ...
Bacillus subtilis
Protein Eng.
16
615-621
2003
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5
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3
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1
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1
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5
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1
-
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1
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657223
Suzuki
Cold-adaptation mechanism of m ...
Thermus thermophilus
Protein Eng.
15
471-476
2002
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9
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30
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2
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1
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21
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1
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1
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9
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30
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1
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21
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639156
Suzuki
Adaptation of a thermophilic e ...
Thermus thermophilus
Protein Eng.
14
85-91
2001
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1
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4
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20
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2
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1
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3
20
-
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1
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1
1
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4
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20
-
-
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1
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3
20
-
-
-
-
-
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-
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639160
Qu
Design, X-ray crystallography, ...
Thermus thermophilus
Acta Crystallogr. Sect. D
57
225-232
2001
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1
6
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2
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1
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7
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1
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1
1
6
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1
-
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7
-
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-
-
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725307
Miyazaki
Ancestral residues stabilizing ...
Sulfolobus tokodaii, Sulfolobus tokodaii 7
J. Biochem.
129
777-782
2001
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1
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7
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1
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5
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1
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7
-
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1
-
-
-
-
-
-
5
-
-
-
-
-
-
-
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1
1
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639161
Matsunami
Overproduction and substrate s ...
Acidithiobacillus ferrooxidans
Biosci. Biotechnol. Biochem.
62
372-373
1998
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-
-
-
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6
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1
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1
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7
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6
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1
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1
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6
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1
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1
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-
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7
-
-
-
-
6
-
-
-
-
-
-
-
-
-
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639166
Imada
Structure of 3-isopropylmalate ...
Acidithiobacillus ferrooxidans
Structure
6
971-982
1998
-
-
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1
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2
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1
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1
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1
1
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1
-
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677302
Suzuki
Crystallization and preliminar ...
Sulfolobus sp. 7, Sulfolobus sp.
Acta Crystallogr. Sect. D
54
444-445
1998
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1
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16
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1
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-
-
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-
-
-
-
-
-
-
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-
-
-
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639153
Wallon
Crystal structures of Escheric ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Thermus thermophilus
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266
1016-1031
1997
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2
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3
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3
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3
2
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-
-
-
-
-
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-
-
-
-
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3
-
-
-
-
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-
-
-
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-
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-
-
639157
Aoshima
Stabilization of Escherichia c ...
Escherichia coli, Thermus thermophilus
Protein Eng.
10
249-254
1997
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1
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1
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1
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3
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2
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2
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639158
Akanuma
Effect of polar side chains at ...
Thermus thermophilus
FEBS Lett.
410
141-144
1997
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4
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10
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1
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8
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639164
Wallon
Purification, catalytic proper ...
Escherichia coli, Thermus thermophilus
Biochim. Biophys. Acta
1337
105-112
1997
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9
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9
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2
1
5
8
1
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639168
Tsuchiya
Crystal structure of 3-isoprop ...
Bacillus coagulans
J. Biochem.
122
1092-1104
1997
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1
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1
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680429
Suzuki
Molecular and phylogenetic cha ...
Sulfolobus tokodaii 7, Sulfolobus tokodaii
J. Bacteriol.
179
1174-1179
1997
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1
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1
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7
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7
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13
1
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7
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639159
Chen
Redesigning secondary structur ...
Thermus thermophilus
Proc. Natl. Acad. Sci. USA
93
12171-12176
1996
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2
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7
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12
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7
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1
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12
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639155
Dean
The role of glutamate 87 in th ...
Thermus thermophilus
Protein Sci.
4
2156-2167
1995
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2
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3
24
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3
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1
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21
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1
24
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5
1
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21
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-
639162
Yoda
Purification and characterizat ...
Sulfolobus sp., Sulfolobus sp. 7
FEMS Microbiol. Lett.
131
243-247
1995
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2
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1
5
3
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2
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1
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2
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5
3
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1
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4
2
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1
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2
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1
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-
639167
Moriyma
-
The crystal structures of muta ...
Thermus thermophilus
J. Biochem.
117(2)
408-413
1995
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1
2
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1
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1
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639165
Hurley
Structure of 3-isopropylmalate ...
Thermus thermophilus
Structure
2
1007-1016
1994
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1
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1
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639163
Kawaguchi
3-Isopropylmalate dehydrogenas ...
Acidithiobacillus ferrooxidans
J. Biochem.
114
370-377
1993
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4
4
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4
3
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5
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1
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3
1
1
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1
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1
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1
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1
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4
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4
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4
3
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1
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1
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3
1
1
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1
-
1
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-
639130
Onodera
Crystallization and preliminar ...
Bacillus subtilis, Thermus thermophilus
J. Biochem.
109
1-2
1991
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2
2
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6
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2
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2
2
2
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2
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-
639131
Yamada
Purification, catalytic proper ...
Thermus thermophilus, Thermus thermophilus HB-8
J. Biochem.
108
449-456
1990
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1
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1
1
2
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5
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12
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1
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1
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2
2
1
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2
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2
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1
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1
1
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1
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1
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2
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5
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1
1
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2
2
1
-
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2
-
2
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-
-
-
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-
-
-
-
639132
Inagaki
-
Cloning and expression of the ...
Acidithiobacillus ferrooxidans
J. Ferment. Bioeng.
70
71-74
1990
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1
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1
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1
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1
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1
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-
-
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-
-
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-
639133
Kakinuma
-
Stereochemistry of the decarbo ...
Thermus thermophilus, Thermus thermophilus HB-8
J. Chem. Soc. Chem. Commun.
1989
1190-1192
1989
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10
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2
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639134
Kohlhaw
beta-Isopropylmalate dehydroge ...
Saccharomyces cerevisiae
Methods Enzymol.
166
429-435
1988
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1
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2
1
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4
1
1
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2
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1
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1
1
2
2
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1
1
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1
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1
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4
1
1
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1
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1
1
2
2
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1
1
-
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-
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-
-
-
-
639135
Okumura
-
Cloning of the beta-isopropylm ...
Acetobacter aceti, Acetobacter aceti No 1023
Agric. Biol. Chem.
52
3125-3129
1988
-
1
1
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2
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1
1
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2
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-
639136
Katsube
Crystallization and preliminar ...
Thermus thermophilus
J. Biochem.
104
679-680
1988
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1
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3
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1
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-
1
-
-
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-
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-
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-
-
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-
639137
Jenkinson
-
Cloning and expression of the ...
Candida albicans
FEMS Microbiol. Lett.
49
285-288
1988
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1
1
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1
1
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1
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-
-
-
-
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-
-
-
-
639154
Searles
Permeabilized cell and radioch ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium
Methods Enzymol.
166
225-229
1988
-
-
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2
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1
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2
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1
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2
-
-
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-
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-
-
-
-
-
639138
Hamasawa
Molecular cloning and nucleoti ...
Cyberlindnera jadinii, Saccharomyces cerevisiae, Thermus thermophilus
J. Gen. Microbiol.
133
1089-1097
1987
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-
1
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1
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8
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3
3
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3
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1
3
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1
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-
-
3
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639152
Croft
Expression of leucine genes fr ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Thermus thermophilus, Thermus thermophilus HB-8
Mol. Gen. Genet.
210
490-497
1987
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1
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12
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4
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3
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1
3
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639139
Sekiguchi
-
Cloning and DNA homology of 3- ...
Bacillus caldolyticus, Bacillus caldotenax, Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae, Thermus thermophilus
FEMS Microbiol. Lett.
36
41-45
1986
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-
2
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6
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6
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6
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2
6
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6
-
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-
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-
-
-
-
639140
Sekiguchi
-
DNA and amino-acid sequences o ...
Bacillus coagulans, Bacillus subtilis, Saccharomyces cerevisiae, Thermus thermophilus
Biochim. Biophys. Acta
867
36-44
1986
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-
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1
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4
-
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4
1
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4
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4
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1
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4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639141
Urano
-
Restriction mapping and partia ...
Citrobacter freundii, Citrobacter freundii TIT 01010
J. Biotechnol.
2
39-45
1985
-
1
1
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3
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2
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1
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1
1
1
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-
2
-
-
-
-
-
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-
-
-
-
-
-
-
-
639142
Karube
-
Cloning and expression of Citr ...
Citrobacter freundii, Citrobacter freundii TIT 01010
Appl. Microbiol. Biotechnol.
20
340-343
1984
-
-
1
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3
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2
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1
1
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-
2
-
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-
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-
-
-
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-
-
-
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639143
Sekiguchi
Cloning of beta-isopropylmalat ...
Bacillus coagulans
Biochim. Biophys. Acta
788
267-273
1984
-
-
1
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-
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6
-
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4
3
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4
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1
-
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-
1
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1
1
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2
-
1
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1
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1
1
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6
-
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4
3
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1
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1
-
1
1
-
-
2
-
1
-
-
-
-
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-
-
-
-
639144
Kato
-
Molecular cloning and Expressi ...
Bacillus sp., Bacillus sp. no 38-2
J. Ferment. Technol.
62
77-80
1984
-
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1
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2
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2
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1
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1
1
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-
2
-
-
-
-
-
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-
-
-
-
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-
-
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639145
Ishi
-
Molecular cloning of beta-isop ...
Clostridium butyricum, Clostridium butyricum M588
Agric. Biol. Chem.
47
2313-2317
1983
-
-
1
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2
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2
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1
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1
1
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2
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-
-
-
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-
-
-
-
639146
Tanaka
Cloning of 3-isopropylmalate d ...
Thermus thermophilus, Thermus thermophilus HB-8
Biochemistry
89
677-682
1981
-
-
1
-
-
-
-
-
-
-
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2
-
14
-
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1
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1
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4
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2
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1
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1
1
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2
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1
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1
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4
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2
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639147
Hsu
Leucine biosynthesis in Saccha ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae SK103
J. Biol. Chem.
255
7255-7260
1980
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639148
Parsons
-
beta-Isopropylmalate dehydroge ...
Salmonella enterica subsp. enterica serovar Typhimurium
Methods Enzymol.
17A
793-799
1970
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2
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639149
Rabin
Isoenzymes of beta-isopropylma ...
Pseudomonas aeruginosa
Arch. Biochem. Biophys.
134
259-261
1969
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639150
Parsons
Purification and properties of ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Biol. Chem.
244
996-1003
1969
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7
2
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639151
Burns
The biosythesis of leucine. II ...
Salmonella enterica subsp. enterica serovar Typhimurium
Biochemistry
2
1053-1058
1963
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