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Literature summary for 1.1.1.84 extracted from

  • Magee, P.T.; Snell, E.E.
    The bacterial degradation of pantothenic acid. IV. Enzymatic conversion of aldopantoate to alpha-ketoisovalerate (1966), Biochemistry, 5, 409-416.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15 0.2 3,3-dimethyl-D-malate
-
 Pseudomonas sp.
0.27
-
 NAD+
-
 Pseudomonas sp.
0.33
-
 3-(n-Propyl)malate
-
 Pseudomonas sp.
8.9 11 D-malate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ Mn2+ or Co2+ required Pseudomonas sp.
K+ K+ or NH4+ required Pseudomonas sp.
Mn2+ Mn2+ or Co2+ required Pseudomonas sp.
NH4+ K+ or NH4+ required Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3,3-dimethylmalate + NAD+ Pseudomonas sp.
-
 3-methyl-2-oxobutanoate + CO2 + NADH
-
 r
(R)-3,3-dimethylmalate + NAD+ Pseudomonas sp. P-2
-
 3-methyl-2-oxobutanoate + CO2 + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 P-2
-
Pseudomonas sp. P-2
-
 P-2
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
50 days, 0°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3,3-dimethylmalate + NAD+
-
 Pseudomonas sp. 3-methyl-2-oxobutanoate + CO2 + NADH
-
 r
(R)-3,3-dimethylmalate + NAD+
-
 Pseudomonas sp. P-2 3-methyl-2-oxobutanoate + CO2 + NADH
-
 r
3-(n-propyl)malate + NAD+
-
 Pseudomonas sp. 2-oxopentanoate + CO2 + NADH
-
 ?
3-(n-propyl)malate + NAD+
-
 Pseudomonas sp. P-2 2-oxopentanoate + CO2 + NADH
-
 ?
D-malate + NAD+
-
 Pseudomonas sp. pyruvate + CO2 + NADH
-
 ?
D-malate + NAD+
-
 Pseudomonas sp. P-2 pyruvate + CO2 + NADH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 5 min, 40% loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 substrates 3,3-dimethylmalate + NAD+ Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas sp.