BRENDA - Enzyme Database show
show all sequences of 1.1.1.84

The bacterial degradation of pantothenic acid. IV. Enzymatic conversion of aldopantoate to alpha-ketoisovalerate

Magee, P.T.; Snell, E.E.; Biochemistry 5, 409-416 (1966)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
0.2
3,3-dimethyl-D-malate
-
Pseudomonas sp.
0.27
-
NAD+
-
Pseudomonas sp.
0.33
-
3-(n-Propyl)malate
-
Pseudomonas sp.
8.9
11
D-malate
-
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
Mn2+ or Co2+ required
Pseudomonas sp.
K+
K+ or NH4+ required
Pseudomonas sp.
Mn2+
Mn2+ or Co2+ required
Pseudomonas sp.
NH4+
K+ or NH4+ required
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3,3-dimethylmalate + NAD+
Pseudomonas sp.
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
Pseudomonas sp.
r
(R)-3,3-dimethylmalate + NAD+
Pseudomonas sp. P-2
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
Pseudomonas sp. P-2
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
P-2
-
Pseudomonas sp. P-2
-
P-2
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1
-
-
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
50 days, 0°C
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3,3-dimethylmalate + NAD+
-
207980
Pseudomonas sp.
3-methyl-2-oxobutanoate + CO2 + NADH
-
207980
Pseudomonas sp.
r
(R)-3,3-dimethylmalate + NAD+
-
207980
Pseudomonas sp. P-2
3-methyl-2-oxobutanoate + CO2 + NADH
-
207980
Pseudomonas sp. P-2
r
3-(n-propyl)malate + NAD+
-
207980
Pseudomonas sp.
2-oxopentanoate + CO2 + NADH
-
207980
Pseudomonas sp.
-
3-(n-propyl)malate + NAD+
-
207980
Pseudomonas sp. P-2
2-oxopentanoate + CO2 + NADH
-
207980
Pseudomonas sp. P-2
-
D-malate + NAD+
-
207980
Pseudomonas sp.
pyruvate + CO2 + NADH
-
207980
Pseudomonas sp.
-
D-malate + NAD+
-
207980
Pseudomonas sp. P-2
pyruvate + CO2 + NADH
-
207980
Pseudomonas sp. P-2
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
5 min, 40% loss of activity
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
substrates 3,3-dimethylmalate + NAD+
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
0.2
3,3-dimethyl-D-malate
-
Pseudomonas sp.
0.27
-
NAD+
-
Pseudomonas sp.
0.33
-
3-(n-Propyl)malate
-
Pseudomonas sp.
8.9
11
D-malate
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
Mn2+ or Co2+ required
Pseudomonas sp.
K+
K+ or NH4+ required
Pseudomonas sp.
Mn2+
Mn2+ or Co2+ required
Pseudomonas sp.
NH4+
K+ or NH4+ required
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-3,3-dimethylmalate + NAD+
Pseudomonas sp.
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
Pseudomonas sp.
r
(R)-3,3-dimethylmalate + NAD+
Pseudomonas sp. P-2
-
3-methyl-2-oxobutanoate + CO2 + NADH
-
Pseudomonas sp. P-2
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1
-
-
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
50 days, 0°C
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3,3-dimethylmalate + NAD+
-
207980
Pseudomonas sp.
3-methyl-2-oxobutanoate + CO2 + NADH
-
207980
Pseudomonas sp.
r
(R)-3,3-dimethylmalate + NAD+
-
207980
Pseudomonas sp. P-2
3-methyl-2-oxobutanoate + CO2 + NADH
-
207980
Pseudomonas sp. P-2
r
3-(n-propyl)malate + NAD+
-
207980
Pseudomonas sp.
2-oxopentanoate + CO2 + NADH
-
207980
Pseudomonas sp.
-
3-(n-propyl)malate + NAD+
-
207980
Pseudomonas sp. P-2
2-oxopentanoate + CO2 + NADH
-
207980
Pseudomonas sp. P-2
-
D-malate + NAD+
-
207980
Pseudomonas sp.
pyruvate + CO2 + NADH
-
207980
Pseudomonas sp.
-
D-malate + NAD+
-
207980
Pseudomonas sp. P-2
pyruvate + CO2 + NADH
-
207980
Pseudomonas sp. P-2
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
5 min, 40% loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
substrates 3,3-dimethylmalate + NAD+
Pseudomonas sp.
Other publictions for EC 1.1.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
207978
Lähdesmäki
Comparison of D-malate and bet ...
Pseudomonas fluorescens
Biochim. Biophys. Acta
613
266-274
1980
-
-
-
-
-
-
2
3
-
-
2
2
-
2
-
-
-
-
-
-
1
-
4
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
3
-
-
2
2
-
-
-
-
-
-
1
-
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207979
Mäntsälä
Purification of pantoate and d ...
Pseudomonas fluorescens
Biochim. Biophys. Acta
526
25-33
1978
-
-
-
-
-
-
-
-
-
-
3
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
3
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207980
Magee
The bacterial degradation of p ...
Pseudomonas sp., Pseudomonas sp. P-2
Biochemistry
5
409-416
1966
-
-
-
-
-
-
-
4
-
4
-
2
-
2
-
-
1
-
-
-
1
1
6
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
4
-
2
-
-
-
1
-
-
1
1
6
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-