BRENDA - Enzyme Database show
show all sequences of 1.1.1.82

The autoinhibition of sorghum NADP malate dehydrogenase is mediated by a C-terminal negative charge

Ruelland, E.; Johansson, K.; Decottignies, P.; Djukic, N.; Miginiac-Maslow, M.; J. Biol. Chem. 273, 33482-33488 (1998)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
thioredoxin
activated by thiol/disulfide interchange with reduced thioredoxin
Sorghum sp.
Engineering
Amino acid exchange
Commentary
Organism
C207A
strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate
Sorghum sp.
C207A/DETAEV
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
C207A/E387Q
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
C29S
strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate
Sorghum sp.
C29S/C207A
no spontaneous activity, activated much faster than the wild-type protein, strictly dependent on reduced thioredoxin for activation
Sorghum sp.
C29S/C207A/DELTAEV
high spontaneous activity, activated by reduced thioredoxin almost instantaneously and also by dithiothreitol alone, although at a much slower rate. The Km-values for both the oxidized and reduced enzyme show no significant differences in the apparent affinity for NADPH, whereas the Km for oxaloacetate is dramatically increased in the oxidized form
Sorghum sp.
C29S/C207A/E387Q
high spontaneous activity, activated by reduced thioredoxin almost instantaneously and also by dithiothreitol alone, although at a much slower rate. The Km-values for both the oxidized and reduced enzyme show no significant differences in the apparent affinity for NADPH, whereas the Km for oxaloacetate is dramatically increased in the oxidized form
Sorghum sp.
C29S/DELTAEV
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
C39S/E387Q
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
DELTAEV
mutant with the two most C-terminal residues deleted, NADP+ does not inhibit activation, activation time course of thioredoxin-dependent activation of both mutant proteins is similar to that of the wild-type protein
Sorghum sp.
E387Q
NADP+ does not inhibit activation, activation time course of thioredoxin-dependent activation of both mutant proteins is similar to that of the wild-type protein
Sorghum sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADPH
mutant enzyme C207A/E387Q, oxidized form
Sorghum sp.
0.031
-
NADPH
mutant enzyme C29S/C207A/E387Q, oxidized enzyme form
Sorghum sp.
0.035
-
NADPH
mutant enzyme C29S/C207A/DELTAEV, oxidized form
Sorghum sp.
0.044
-
NADPH
mutant enzyme C29S/E387Q, reduced form
Sorghum sp.
0.046
-
NADPH
mutant enzyme C29S/E387Q, reduced form
Sorghum sp.
0.047
-
NADPH
mutant enzyme C207A/DELTAEV, oxidized form
Sorghum sp.
0.049
-
NADPH
mutant enzyme C29S/DELTAEV, oxidized form
Sorghum sp.
0.05
-
oxaloacetate
mutant enzyme C39S/E387Q, reduced form
Sorghum sp.
0.054
-
oxaloacetate
mutant enzyme C207A/DELTAEV, reduced form
Sorghum sp.
0.056
-
oxaloacetate
mutant enzyme C29S/E387Q, reduced form
Sorghum sp.
0.06
-
NADPH
mutant enzyme C207A/DELTAEV, and mutant enzyme C29S/C207S/DELTAEV, reduced forms
Sorghum sp.
0.063
-
NADPH
mutant enzyme C29S/DELTAEV, reduced form
Sorghum sp.
0.066
-
NADPH
mutant enzyme C29S/C207A/E387Q, reduced form and mutant enzyme C29S/C207A/DELTAEV, reduced form
Sorghum sp.
0.07
-
oxaloacetate
mutant enzyme C29S/DELTAEV, reduced form
Sorghum sp.
0.086
-
oxaloacetate
mutant enzyme C29S/C207A/E387Q, reduced form
Sorghum sp.
0.094
-
oxaloacetate
mutant enzyme C29S/C207A, reduced form
Sorghum sp.
0.115
-
NADPH
mutant enzyme C29S/C207A, reduced form
Sorghum sp.
0.447
-
oxaloacetate
mutant enzyme C207A/DELTAEV, oxidized form
Sorghum sp.
0.578
-
oxaloacetate
mutant enzyme C29S/DELTAEV, oxidized form
Sorghum sp.
0.583
-
oxaloacetate
mutant enzyme C29S/E387Q, oxidized form
Sorghum sp.
0.894
-
oxaloacetate
mutant enzyme C29S/C207A/E387Q, oxidized form
Sorghum sp.
0.931
-
oxaloacetate
mutant enzyme C29S/C207A/DELTAEV, oxidized form
Sorghum sp.
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Sorghum sp.
9507
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sorghum sp.
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
oxaloacetate + NADPH
-
287422
Sorghum sp.
(S)-malate + NADP+
-
287422
Sorghum sp.
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
thioredoxin
activated by thiol/disulfide interchange with reduced thioredoxin
Sorghum sp.
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C207A
strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate
Sorghum sp.
C207A/DETAEV
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
C207A/E387Q
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
C29S
strictly thioredoxin-dependent, totally insensitive to diethyl dicarbonate
Sorghum sp.
C29S/C207A
no spontaneous activity, activated much faster than the wild-type protein, strictly dependent on reduced thioredoxin for activation
Sorghum sp.
C29S/C207A/DELTAEV
high spontaneous activity, activated by reduced thioredoxin almost instantaneously and also by dithiothreitol alone, although at a much slower rate. The Km-values for both the oxidized and reduced enzyme show no significant differences in the apparent affinity for NADPH, whereas the Km for oxaloacetate is dramatically increased in the oxidized form
Sorghum sp.
C29S/C207A/E387Q
high spontaneous activity, activated by reduced thioredoxin almost instantaneously and also by dithiothreitol alone, although at a much slower rate. The Km-values for both the oxidized and reduced enzyme show no significant differences in the apparent affinity for NADPH, whereas the Km for oxaloacetate is dramatically increased in the oxidized form
Sorghum sp.
C29S/DELTAEV
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
C39S/E387Q
very low spontaneous activity, high Km-value for oxaloacetate, fast activation kinetics in the presence of reduced thioredoxin, ability to be activated by dithiothreitol alone at a slow rate
Sorghum sp.
DELTAEV
mutant with the two most C-terminal residues deleted, NADP+ does not inhibit activation, activation time course of thioredoxin-dependent activation of both mutant proteins is similar to that of the wild-type protein
Sorghum sp.
E387Q
NADP+ does not inhibit activation, activation time course of thioredoxin-dependent activation of both mutant proteins is similar to that of the wild-type protein
Sorghum sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADPH
mutant enzyme C207A/E387Q, oxidized form
Sorghum sp.
0.031
-
NADPH
mutant enzyme C29S/C207A/E387Q, oxidized enzyme form
Sorghum sp.
0.035
-
NADPH
mutant enzyme C29S/C207A/DELTAEV, oxidized form
Sorghum sp.
0.044
-
NADPH
mutant enzyme C29S/E387Q, reduced form
Sorghum sp.
0.046
-
NADPH
mutant enzyme C29S/E387Q, reduced form
Sorghum sp.
0.047
-
NADPH
mutant enzyme C207A/DELTAEV, oxidized form
Sorghum sp.
0.049
-
NADPH
mutant enzyme C29S/DELTAEV, oxidized form
Sorghum sp.
0.05
-
oxaloacetate
mutant enzyme C39S/E387Q, reduced form
Sorghum sp.
0.054
-
oxaloacetate
mutant enzyme C207A/DELTAEV, reduced form
Sorghum sp.
0.056
-
oxaloacetate
mutant enzyme C29S/E387Q, reduced form
Sorghum sp.
0.06
-
NADPH
mutant enzyme C207A/DELTAEV, and mutant enzyme C29S/C207S/DELTAEV, reduced forms
Sorghum sp.
0.063
-
NADPH
mutant enzyme C29S/DELTAEV, reduced form
Sorghum sp.
0.066
-
NADPH
mutant enzyme C29S/C207A/E387Q, reduced form and mutant enzyme C29S/C207A/DELTAEV, reduced form
Sorghum sp.
0.07
-
oxaloacetate
mutant enzyme C29S/DELTAEV, reduced form
Sorghum sp.
0.086
-
oxaloacetate
mutant enzyme C29S/C207A/E387Q, reduced form
Sorghum sp.
0.094
-
oxaloacetate
mutant enzyme C29S/C207A, reduced form
Sorghum sp.
0.115
-
NADPH
mutant enzyme C29S/C207A, reduced form
Sorghum sp.
0.447
-
oxaloacetate
mutant enzyme C207A/DELTAEV, oxidized form
Sorghum sp.
0.578
-
oxaloacetate
mutant enzyme C29S/DELTAEV, oxidized form
Sorghum sp.
0.583
-
oxaloacetate
mutant enzyme C29S/E387Q, oxidized form
Sorghum sp.
0.894
-
oxaloacetate
mutant enzyme C29S/C207A/E387Q, oxidized form
Sorghum sp.
0.931
-
oxaloacetate
mutant enzyme C29S/C207A/DELTAEV, oxidized form
Sorghum sp.
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Sorghum sp.
9507
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
oxaloacetate + NADPH
-
287422
Sorghum sp.
(S)-malate + NADP+
-
287422
Sorghum sp.
?
Other publictions for EC 1.1.1.82
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735713
Dulermo
Analysis of ATP-citrate lyase ...
no activity in Yarrowia lipolytica
Biochim. Biophys. Acta
1851
1107-1117
2015
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739249
Heyno
Putative role of the malate va ...
Arabidopsis thaliana
Philos. Trans. R. Soc. Lond. B Biol. Sci.
369
20130228
2014
1
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5
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723892
Libik-Konieczny
-
Pathogen-induced changes in ma ...
Mesembryanthemum crystallinum
Acta Physiol. Plant.
34
1471-1477
2012
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1
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2
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2
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696461
Kawakami
Refolding, characterization an ...
Aeropyrum pernix
Biochim. Biophys. Acta
1794
1496-1504
2009
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1
1
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6
-
-
3
-
-
1
-
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1
-
1
-
-
-
3
2
1
1
3
6
1
-
1
2
-
-
-
-
-
1
2
1
-
-
-
-
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6
-
-
3
-
-
-
-
1
1
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3
2
1
1
3
6
1
-
1
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-
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689663
Anderson
Distribution of thioredoxins f ...
Pisum sativum
Plant Sci.
174
432-445
2008
1
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1
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1
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682850
Zhang
-
Characteristics of ribulose-1, ...
Oryza sativa
S. Afr. J. Bot.
73
22-28
2007
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2
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1
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1
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1
1
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1
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1
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1
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688456
Hameister
Transcriptional regulation of ...
Arabidopsis lyrata subsp. petraea, Arabidopsis thaliana, Capsella bursa-pastoris, Capsella rubella, Cochlearia officinalis, Lepidium densiflorum, Lepidium latifolium
J. Mol. Evol.
65
437-455
2007
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7
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11
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7
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7
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7
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667200
Rondeau
NADP-malate dehydrogenase gene ...
Chrysopogon zizanioides, Dichanthium aristatum, Flaveria trinervia, Heteropogon contortus, Hyparrhenia rufa, Ischaemum koleostachys, Megathyrsus maximus, Melinis repens, Oplismenus compositus, Oryza sativa, Paspalidium geminatum, Paspalum paniculatum, Pogonatherum paniceum, Saccharum hybrid cultivar R570, Saccharum officinarum, Saccharum spontaneum, Sorghum arundinaceum, Sorghum bicolor, Themeda quadrivalvis, Zea mays
Ann. Bot.
96
1307-1314
2005
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31
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23
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23
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670586
Lemaire
NADP-malate dehydrogenase from ...
Chlamydomonas reinhardtii
Plant Physiol.
137
514-521
2005
1
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2
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5
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657015
Gomez
NADP-malate dehydrogenase from ...
Chlamydomonas reinhardtii
Plant Mol. Biol.
48
211-221
2002
2
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1
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5
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1
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287424
Schepens
The dimer contact area of sorg ...
Sorghum sp.
FEBS Lett.
471
240-244
2000
1
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3
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1
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2
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1
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287425
Hirasawa
Oxidation-reduction properties ...
Sorghum bicolor, Sorghum sp.
Biochemistry
39
3344-3350
2000
2
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2
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2
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2
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2
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2
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287395
Krimm
Direct NMR observation of the ...
Sorghum bicolor
J. Biol. Chem.
274
34539-34542
1999
1
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1
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2
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1
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287414
Johansson
Structural basis for light act ...
Sorghum bicolor
Biochemistry
38
4319-4326
1999
1
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1
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3
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287423
Carr
Chloroplast NADP-malate dehydr ...
Flaveria bidentis
Structure
7
461-475
1999
1
-
-
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-
-
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-
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287415
MacPherson
Crystallization and preliminar ...
Flaveria bidentis
Acta Crystallogr. Sect. D
54
654-656
1998
-
-
-
1
-
-
-
-
1
-
-
-
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1
-
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1
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-
-
-
-
-
-
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287422
Ruelland
The autoinhibition of sorghum ...
Sorghum sp.
J. Biol. Chem.
273
33482-33488
1998
1
-
-
-
11
-
-
22
1
-
-
-
-
2
-
-
-
-
-
-
-
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1
-
-
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-
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-
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-
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-
-
-
-
-
-
-
-
-
287419
Riessland
Determination of the regulator ...
Pisum sativum
Biol. Chem.
378
983-988
1997
2
-
-
-
5
-
-
19
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
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-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287427
Braun
Kinetic evidence for protein c ...
Glycine max
Eur. J. Biochem.
240
781-788
1996
1
-
-
-
-
-
-
-
3
-
-
-
-
5
-
-
-
-
-
4
-
-
1
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1
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-
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-
-
287428
Lemaire
The catalytic site of chloropl ...
Sorghum sp.
Eur. J. Biochem.
236
947-952
1996
-
-
-
-
4
-
-
9
1
-
-
-
-
3
-
-
-
-
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-
-
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2
-
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-
-
6
1
-
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9
1
-
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2
-
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6
1
-
-
-
-
-
-
-
-
-
287417
Scheibe
Analysis of biophysical differ ...
Pisum sativum, Spinacia oleracea
Arch. Biochem. Biophys.
300
635-640
1993
-
-
-
-
-
-
3
-
-
-
-
-
-
2
-
-
-
-
-
2
-
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-
-
1
4
-
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-
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2
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4
-
-
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-
-
-
-
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-
-
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287386
Agostino
Amino acid sequence and molecu ...
Zea mays
Plant Physiol.
98
1506-1510
1992
-
-
-
-
-
-
-
-
-
-
3
1
-
4
-
-
1
-
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2
1
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-
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-
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287418
Kampfenkel
Limited proteolysis of NADP-ma ...
Pisum sativum
Biochim. Biophys. Acta
1156
71-77
1992
-
-
-
-
-
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1
-
2
-
-
2
-
-
1
-
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2
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-
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-
-
-
287421
Issakidis
Site-directed mutagenesis reve ...
Sorghum sp.
J. Biol. Chem.
267
21577-21583
1992
2
-
1
-
4
-
-
10
1
-
2
-
-
3
-
-
1
-
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2
-
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1
1
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2
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1
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4
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1
1
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287426
Jacquot
Properties of recombinant NADP ...
Sorghum bicolor
Eur. J. Biochem.
199
47-51
1991
-
-
1
-
-
-
-
4
-
-
-
-
-
6
-
-
-
-
-
2
-
-
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1
-
-
-
-
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-
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-
-
-
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-
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-
-
1
1
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-
-
1
-
-
-
-
-
-
-
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-
287385
Luchetta
Structure and characterization ...
Sorghum bicolor
Gene
89
171-177
1990
-
-
1
-
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-
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-
-
1
-
4
-
-
-
-
-
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-
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2
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
287387
Vairinhos
-
Purification and molecular for ...
Echinochloa crus-galli
Plant Sci.
71
173-177
1990
-
-
-
-
-
-
-
-
-
-
5
1
-
1
-
-
1
-
-
-
2
-
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1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
5
1
-
-
-
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-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287388
Miginiac-Maslow
-
Regulation of corn leaf NADP-m ...
Pisum sativum, Spinacia oleracea, Zea mays
Biochim. Biophys. Acta
1017
273-279
1990
-
-
-
-
-
-
3
-
3
-
-
-
-
3
-
-
-
-
-
-
-
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-
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-
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-
-
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-
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-
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-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287389
Scheibe
Structural and catalytic prope ...
Pisum sativum
Eur. J. Biochem.
189
581-587
1990
1
-
-
-
-
1
-
-
1
-
-
-
-
2
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
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-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287413
Cretin
Primary structure of sorghum m ...
Sorghum bicolor
Eur. J. Biochem.
192
299-303
1990
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287420
Gupta
-
Properties of NADP+-malate deh ...
Cicer arietinum
Plant Physiol. Biochem.
28
671-678
1990
-
-
-
-
-
-
7
4
-
-
1
1
-
1
-
-
1
-
-
1
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
4
-
-
1
1
-
-
-
1
-
1
1
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287390
Cheng
Photosynthesis in Flaveria bro ...
Flaveria brownii
Plant Physiol.
87
867-873
1988
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
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-
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-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287391
Migniac-Maslow
Effect of high light intensiti ...
Spinacia oleracea
Planta
173
468-473
1988
1
-
-
-
-
-
1
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
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1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287392
Fickenscher
Limited proteolysis of inactiv ...
Pisum sativum
Arch. Biochem. Biophys.
260
771-779
1988
4
-
-
-
-
2
3
6
1
-
1
-
-
2
-
1
-
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
2
-
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-
6
1
-
1
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287393
Kagawa
NADP-malate dehydrogenase from ...
Zea mays
Arch. Biochem. Biophys.
260
674-695
1988
1
-
-
-
-
-
-
8
-
-
3
1
-
2
-
-
1
-
-
1
1
-
5
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
8
-
-
3
1
-
-
-
1
-
1
1
-
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287394
Jawali
-
Presence of essential histidin ...
Zea mays
Phytochemistry
26
1859-1862
1987
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287396
Scheibe
-
NADP-malate dehydrogenase acti ...
Spinacia oleracea
J. Plant Physiol.
124
103-110
1986
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287397
Jacquot
-
Further evidence for a role of ...
Zea mays
FEBS Lett.
209
87-91
1986
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287398
Nakamoto
Light activation of pyruvate, ...
Zea mays
Plant Physiol.
82
312-315
1986
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287400
Scheibe
-
Studies on the mechanism of th ...
Pisum sativum
Biochim. Biophys. Acta
870
191-197
1986
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287401
Ferte
Structural, immunological and ...
Spinacia oleracea, Zea mays
Eur. J. Biochem.
154
587-595
1986
-
-
-
-
-
-
-
-
2
-
5
-
-
5
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287399
Edwards
-
Pyruvate, Pi dikinase and NADP ...
Pisum sativum, Zea mays
Annu. Rev. Plant Physiol.
36
255-286
1985
2
-
-
-
-
-
-
4
2
-
2
-
-
2
-
-
-
-
-
2
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
2
-
2
-
-
-
-
-
-
2
-
-
2
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287402
Gotow
Light activation of NADP-malat ...
Vicia faba
Plant Physiol.
79
829-832
1985
1
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287405
Scheibe
-
The dark (oxidized) form of th ...
Pisum sativum
FEBS Lett.
180
317-320
1985
1
-
-
-
-
-
2
6
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
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286653
Isegawa
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Submitochondrial location and ...
Euglena gracilis, Euglena gracilis SM-ZK
Agric. Biol. Chem.
48
549-552
1984
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8
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287403
Ferte
-
Purification of several NADP-d ...
Spinacia oleracea
Plant Sci. Lett.
37
115-121
1984
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4
2
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1
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287406
Perrot-Rechenmann
-
Localization of NADP-malate de ...
Zea mays
Plant Sci. Lett.
30
219-226
1983
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1
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287407
Scheibe
NADP regulates the light activ ...
Pisum sativum
Planta
157
548-553
1983
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1
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1
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1
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287409
Fickenscher
-
Purification and properties of ...
Pisum sativum
Biochim. Biophys. Acta
749
249-254
1983
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4
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4
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4
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4
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1
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2
4
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287410
Ferte
-
Molecular properties and thior ...
Spinacia oleracea
FEBS Lett.
146
133-138
1982
1
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3
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1
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1
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287411
Jacquot
Enzyme regulation in C4 photos ...
Zea mays
Plant Physiol.
68
300-304
1981
1
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1
1
2
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1
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287412
Mohamed
Extraction of chloroplast ligh ...
Pisum sativum
Arch. Biochem. Biophys.
209
606-612
1981
1
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287404
Wolosiuk
-
Regulation of NADP-malate dehy ...
Spinacia oleracea
FEBS Lett.
81
253-258
1977
1
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1
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1
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287384
Johnson
NADP-malate dehydrogenase: pho ...
Hordeum vulgare, Spinacia oleracea
Biochem. Biophys. Res. Commun.
43
703-709
1971
2
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4
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287416
Hatch
NADPH-specific malate dehydrog ...
Amaranthus palmeri, Beta vulgaris, Daucus carota, Pisum sativum, Saccharum sp., Spinacia oleracea, Zea mays
Biochem. Biophys. Res. Commun.
34
589-593
1969
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3
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3
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5
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7
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3
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15
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1
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15
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