BRENDA - Enzyme Database show
show all sequences of 1.1.1.81

New insights into the mechanism of substrates trafficking in glyoxylate/hydroxypyruvate reductases

Lassalle, L.; Engilberge, S.; Madern, D.; Vauclare, P.; Franzetti, B.; Girard, E.; Sci. Rep. 6, 20629 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pyrococcus furiosus
expressed in Escherichia coli BL21(DE3) cells
Pyrococcus horikoshii
expressed in Escherichia coli BL21(DE3) cells
Pyrococcus yayanosii
Crystallization (Commentary)
Crystallization
Organism
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
Pyrococcus furiosus
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
Pyrococcus horikoshii
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
Pyrococcus yayanosii
Inhibitors
Inhibitors
Commentary
Organism
Structure
Hydroxypyruvate
-
Pyrococcus furiosus
Hydroxypyruvate
-
Pyrococcus horikoshii
Hydroxypyruvate
-
Pyrococcus yayanosii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
0.07
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
0.09
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
0.14
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
0.165
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
0.41
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hydroxypyruvate + NADH + H+
Pyrococcus horikoshii
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADH + H+
Pyrococcus yayanosii
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADH + H+
Pyrococcus furiosus
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADH + H+
Pyrococcus yayanosii CH1
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus horikoshii
-
D-glycerate + NADP+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus yayanosii
-
D-glycerate + NADP+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus furiosus
-
D-glycerate + NADP+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus yayanosii CH1
-
D-glycerate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
Q8U3Y2
-
-
Pyrococcus horikoshii
-
-
-
Pyrococcus yayanosii
F8AEA4
-
-
Pyrococcus yayanosii CH1
F8AEA4
-
-
Purification (Commentary)
Commentary
Organism
Resource Q column chromatography and Superose 12 column chromatography
Pyrococcus furiosus
Resource Q column chromatography and Superose 12 column chromatography
Pyrococcus horikoshii
Resource Q column chromatography and Superose 12 column chromatography
Pyrococcus yayanosii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus horikoshii
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus yayanosii
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus furiosus
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus yayanosii CH1
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus horikoshii
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus yayanosii
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus furiosus
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus yayanosii CH1
D-glycerate + NADP+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.6
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
1.2
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
4.5
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
6.5
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
10
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
25
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
the enzyme prefers NADH over NADPH
Pyrococcus furiosus
NADH
the enzyme prefers NADH over NADPH
Pyrococcus horikoshii
NADH
the enzyme prefers NADH over NADPH
Pyrococcus yayanosii
NADPH
the enzyme prefers NADH over NADPH
Pyrococcus furiosus
NADPH
the enzyme prefers NADH over NADPH
Pyrococcus horikoshii
NADPH
the enzyme prefers NADH over NADPH
Pyrococcus yayanosii
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.35
-
Hydroxypyruvate
in the presence of NADH, at pH 8.0 and 50°C
Pyrococcus furiosus
0.76
-
Hydroxypyruvate
in the presence of NADH, at pH 8.0 and 50°C
Pyrococcus yayanosii
2
-
Hydroxypyruvate
in the presence of NADPH, at pH 8.0 and 50°C
Pyrococcus yayanosii
3.82
-
Hydroxypyruvate
in the presence of NADH, at pH 8.0 and 50°C
Pyrococcus horikoshii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Pyrococcus furiosus
expressed in Escherichia coli BL21(DE3) cells
Pyrococcus horikoshii
expressed in Escherichia coli BL21(DE3) cells
Pyrococcus yayanosii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
the enzyme prefers NADH over NADPH
Pyrococcus furiosus
NADH
the enzyme prefers NADH over NADPH
Pyrococcus horikoshii
NADH
the enzyme prefers NADH over NADPH
Pyrococcus yayanosii
NADPH
the enzyme prefers NADH over NADPH
Pyrococcus furiosus
NADPH
the enzyme prefers NADH over NADPH
Pyrococcus horikoshii
NADPH
the enzyme prefers NADH over NADPH
Pyrococcus yayanosii
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
Pyrococcus furiosus
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
Pyrococcus horikoshii
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl
Pyrococcus yayanosii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hydroxypyruvate
-
Pyrococcus furiosus
Hydroxypyruvate
-
Pyrococcus horikoshii
Hydroxypyruvate
-
Pyrococcus yayanosii
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.35
-
Hydroxypyruvate
in the presence of NADH, at pH 8.0 and 50°C
Pyrococcus furiosus
0.76
-
Hydroxypyruvate
in the presence of NADH, at pH 8.0 and 50°C
Pyrococcus yayanosii
2
-
Hydroxypyruvate
in the presence of NADPH, at pH 8.0 and 50°C
Pyrococcus yayanosii
3.82
-
Hydroxypyruvate
in the presence of NADH, at pH 8.0 and 50°C
Pyrococcus horikoshii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
0.07
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
0.09
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
0.14
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
0.165
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
0.41
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hydroxypyruvate + NADH + H+
Pyrococcus horikoshii
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADH + H+
Pyrococcus yayanosii
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADH + H+
Pyrococcus furiosus
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADH + H+
Pyrococcus yayanosii CH1
-
D-glycerate + NAD+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus horikoshii
-
D-glycerate + NADP+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus yayanosii
-
D-glycerate + NADP+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus furiosus
-
D-glycerate + NADP+
-
-
?
hydroxypyruvate + NADPH + H+
Pyrococcus yayanosii CH1
-
D-glycerate + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Resource Q column chromatography and Superose 12 column chromatography
Pyrococcus furiosus
Resource Q column chromatography and Superose 12 column chromatography
Pyrococcus horikoshii
Resource Q column chromatography and Superose 12 column chromatography
Pyrococcus yayanosii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus horikoshii
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus yayanosii
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus furiosus
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
-
739674
Pyrococcus yayanosii CH1
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus horikoshii
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus yayanosii
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus furiosus
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
-
739674
Pyrococcus yayanosii CH1
D-glycerate + NADP+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.6
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
1.2
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
4.5
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
6.5
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
10
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
25
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
15
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
30
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
40
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
60
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
65
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
75
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
15
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
30
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
40
-
Hydroxypyruvate
with NADPH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
60
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus yayanosii
65
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus horikoshii
75
-
Hydroxypyruvate
with NADH as cosubstrate, at pH 8.0 and 50°C
Pyrococcus furiosus
Other publictions for EC 1.1.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
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1
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1
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3
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1
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1
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1
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1
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3
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1
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1
1
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739674
Lassalle
New insights into the mechanis ...
Pyrococcus furiosus, Pyrococcus horikoshii, Pyrococcus yayanosii, Pyrococcus yayanosii CH1
Sci. Rep.
6
20629
2016
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3
3
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3
6
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8
-
4
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3
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8
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6
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6
4
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3
6
3
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3
4
6
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8
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3
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8
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6
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6
6
737745
Hoover
Identification of catalyticall ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1834
2663-2671
2013
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1
3
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4
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8
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2
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7
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1
3
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8
-
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1
1
726219
Timm
The hydroxypyruvate-reducing s ...
Arabidopsis thaliana
Plant Physiol.
155
694-705
2011
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1
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1
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2
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1
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2
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2
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1
2
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1
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2
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2
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1
1
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695413
Piotrowska
-
Cellular response of light/dar ...
Chlorella vulgaris
Acta Physiol. Plant.
31
573-585
2009
5
-
-
-
-
-
1
-
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1
-
1
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1
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1
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1
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1
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1
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5
-
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1
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1
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1
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1
-
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1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
705820
Wang
Shoot-specific down-regulation ...
Brassica napus
Mol. Plant
2
191-200
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
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-
-
1
-
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1
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1
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1
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1
-
-
700736
Timm
A cytosolic pathway for the co ...
Arabidopsis thaliana
Plant Cell
20
2848-2859
2008
-
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1
-
1
-
1
-
2
-
1
1
-
6
-
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1
-
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3
1
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2
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2
4
-
2
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2
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2
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1
1
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2
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3
1
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1
1
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684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
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1
1
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-
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4
-
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2
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1
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688165
Shen
Over-expression of a hydroxypy ...
Methylobacterium sp., Methylobacterium sp. MB200
J. Ind. Microbiol. Biotechnol.
34
657-663
2007
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669886
Booth
Structural basis of substrate ...
Homo sapiens, Mus musculus
J. Mol. Biol.
360
178-189
2006
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286510
Hagishita
Immunological characterization ...
Cucumis sativus, Hyphomicrobium methylovorum GM2, Methylobacterium extorquens AM1, Methylobacterium organophilum, Spinacia oleracea
FEMS Microbiol. Lett.
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49-52
1996
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Yoshida
Cloning and expression of the ...
Hyphomicrobium methylovorum GM2
Eur. J. Biochem.
223
727-732
1994
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287365
Oliver
Inhibition of tobacco NADH-hyd ...
Nicotiana tabacum cv. SR1
Mol. Gen. Genet.
239
425-434
1993
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286505
Kleczkowski
Oxalate as a potent and select ...
Spinacia oleracea
Biochem. J.
276
125-127
1991
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286508
Chistoserdova
Purification and characterizat ...
Methylobacterium extorquens AM1
J. Bacteriol.
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7228-7232
1991
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287368
Kleczkowski
Enzymology of the reduction of ...
Hordeum vulgare
Plant Physiol.
94
819-825
1990
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287369
Krema
-
Hydroxypyruvate reductase from ...
Methylobacterium extorquens AM1
Methods Enzymol.
188
373-378
1990
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287370
Kleczkowski
Identification of hydroxypyruv ...
Zea mays
Plant Physiol.
91
278-288
1989
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287371
Murray
Metabolism of hydroxypyruvate ...
Hordeum vulgare
Plant Physiol.
91
395-400
1989
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287372
Sautter
Import of peroxisomal hydroxyp ...
Citrullus lanatus subsp. vulgaris, Cucurbita pepo, Spinacia oleracea
Planta
176
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1988
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287373
Kleczkowski
Subcellular location of NADPH- ...
Pisum sativum, Spinacia oleracea
Plant Physiol.
88
1182-1185
1988
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287374
Kleczkowski
Purification and characterizat ...
Spinacia oleracea
Biochem. J.
250
145-152
1988
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287383
Coderch
Effect of salts on D-glycerate ...
Bos taurus
Biochim. Biophys. Acta
566
21-31
1979
3
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4
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287375
Bamforth
-
Hydroxypyruvate reductase acti ...
Paracoccus denitrificans
J. Gen. Microbiol.
101
259-267
1977
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287376
Feld
Comparison of D-glycerate dehy ...
Bos taurus, Sus scrofa
Arch. Biochem. Biophys.
166
417-425
1975
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3
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287377
Feld
D-Glycerate dehydrogenase from ...
Sus scrofa
Methods Enzymol.
41B
289-293
1975
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287378
Sugimoto
Mechanisms of inhibition and a ...
Bos taurus
J. Biochem.
72
1317-1325
1972
6
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6
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287379
Sugimoto
Purification and properties of ...
Bos taurus
J. Biochem.
72
1307-1315
1972
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287380
Uhr
Glycine and serine inhibition ...
Rattus norvegicus
FEBS Lett.
17
137-140
1971
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Kohn
Tartaric acid metabolism. VII. ...
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243
2494-2499
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287382
Kohn
-
Hydroxypyruvate reductase (D-g ...
Delftia acidovorans
Methods Enzymol.
9
229-232
1966
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