BRENDA - Enzyme Database show
show all sequences of 1.1.1.81

Identification of catalytically important amino acid residues for enzymatic reduction of glyoxylate in plants

Hoover, G.J.; Jorgensen, R.; Rochon, A.; Bajwa, V.S.; Merrill, A.R.; Shelp, B.J.; Biochim. Biophys. Acta 1834, 2663-2671 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene HPR3, sequence comparisons of GLYR genes and HPR genes
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1
-
Hydroxypyruvate
GLYR1, at pH 7.8 and 22C
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
cytosol
-
Arabidopsis thaliana
5829
-
peroxisome
-
Arabidopsis thaliana
5777
-
plastid
-
Arabidopsis thaliana
9536
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glycerate + NAD+
Arabidopsis thaliana
-
hydroxypyruvate + NADH + H+
-
-
?
D-glycerate + NADP+
Arabidopsis thaliana
-
hydroxypyruvate + NADPH + H+
-
-
r
additional information
Arabidopsis thaliana
HPR3 prefers NADPH over NADH and converts glycerate to hydroxypyruvate, the purified recombinant HPR3 shows similar activity with hydroxypyruvate and glyoxylate
?
-
-
-
additional information
Arabidopsis thaliana
the recombinant AtHPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate. Isozyme AtHPR1 also converts glyoxylate to glycolate, albeit with much lower catalytic efficiency than for hydroxypyruvate
?
-
-
-
additional information
Arabidopsis thaliana
the recombinant AtHPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
A0A178WMD4
-
-
Arabidopsis thaliana
A0A1I9LPQ6
-
-
Arabidopsis thaliana
Q9C9W5
-
-
Arabidopsis thaliana
Q9CA90
-
-
Arabidopsis thaliana
Q9LE33
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glycerate + NAD+
-
737745
Arabidopsis thaliana
hydroxypyruvate + NADH + H+
-
-
-
?
D-glycerate + NADP+
-
737745
Arabidopsis thaliana
hydroxypyruvate + NADPH + H+
-
-
-
r
hydroxypyruvate + NADH + H+
-
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
isoform HPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
isoform HPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
isoform HPR3 prefers NADPH over NADH and glyoxylate over hydroxypyvruvate
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH + H+
isoform HPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate
737745
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
isoform HPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
737745
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
isoform HPR3 prefers NADPH over NADH and glyoxylate over hydroxypyvruvate
737745
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
?
additional information
the enzyme GLYR1 has negligible hydroxypyruvate dependent activity with NADPH
737745
Arabidopsis thaliana
?
-
-
-
-
additional information
HPR3 prefers NADPH over NADH and converts glycerate to hydroxypyruvate, the purified recombinant HPR3 shows similar activity with hydroxypyruvate and glyoxylate
737745
Arabidopsis thaliana
?
-
-
-
-
additional information
the recombinant AtHPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate. Isozyme AtHPR1 also converts glyoxylate to glycolate, albeit with much lower catalytic efficiency than for hydroxypyruvate
737745
Arabidopsis thaliana
?
-
-
-
-
additional information
the recombinant AtHPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
737745
Arabidopsis thaliana
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
recombinant HPR3 prefers NADPH over NADH; the recombinant AtHPR2 prefers NADPH over NADH
Arabidopsis thaliana
NAD+
-
Arabidopsis thaliana
NADH
isoform HPR1 prefers NADH over NADPH; isoform HPR2 prefers NADPH over NADH; isoform HPR3 prefers NADPH over NADH
Arabidopsis thaliana
NADP+
;
Arabidopsis thaliana
NADPH
isoform HPR1 prefers NADH over NADPH; isoform HPR2 prefers NADPH over NADH; isoform HPR3 prefers NADPH over NADH
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene HPR3, sequence comparisons of GLYR genes and HPR genes
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the recombinant AtHPR2 prefers NADPH over NADH
Arabidopsis thaliana
additional information
recombinant HPR3 prefers NADPH over NADH
Arabidopsis thaliana
NAD+
-
Arabidopsis thaliana
NADH
isoform HPR2 prefers NADPH over NADH
Arabidopsis thaliana
NADH
; isoform HPR1 prefers NADH over NADPH
Arabidopsis thaliana
NADH
isoform HPR3 prefers NADPH over NADH
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana
NADP+
-
Arabidopsis thaliana
NADPH
; isoform HPR2 prefers NADPH over NADH
Arabidopsis thaliana
NADPH
isoform HPR1 prefers NADH over NADPH
Arabidopsis thaliana
NADPH
isoform HPR3 prefers NADPH over NADH
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1
-
Hydroxypyruvate
GLYR1, at pH 7.8 and 22C
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
cytosol
-
Arabidopsis thaliana
5829
-
peroxisome
-
Arabidopsis thaliana
5777
-
plastid
-
Arabidopsis thaliana
9536
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glycerate + NAD+
Arabidopsis thaliana
-
hydroxypyruvate + NADH + H+
-
-
?
D-glycerate + NADP+
Arabidopsis thaliana
-
hydroxypyruvate + NADPH + H+
-
-
r
additional information
Arabidopsis thaliana
HPR3 prefers NADPH over NADH and converts glycerate to hydroxypyruvate, the purified recombinant HPR3 shows similar activity with hydroxypyruvate and glyoxylate
?
-
-
-
additional information
Arabidopsis thaliana
the recombinant AtHPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate. Isozyme AtHPR1 also converts glyoxylate to glycolate, albeit with much lower catalytic efficiency than for hydroxypyruvate
?
-
-
-
additional information
Arabidopsis thaliana
the recombinant AtHPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glycerate + NAD+
-
737745
Arabidopsis thaliana
hydroxypyruvate + NADH + H+
-
-
-
?
D-glycerate + NADP+
-
737745
Arabidopsis thaliana
hydroxypyruvate + NADPH + H+
-
-
-
r
hydroxypyruvate + NADH + H+
-
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
isoform HPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
isoform HPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADH + H+
isoform HPR3 prefers NADPH over NADH and glyoxylate over hydroxypyvruvate
737745
Arabidopsis thaliana
D-glycerate + NAD+
-
-
-
?
hydroxypyruvate + NADPH + H+
isoform HPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate
737745
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
isoform HPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
737745
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
?
hydroxypyruvate + NADPH + H+
isoform HPR3 prefers NADPH over NADH and glyoxylate over hydroxypyvruvate
737745
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
?
additional information
the enzyme GLYR1 has negligible hydroxypyruvate dependent activity with NADPH
737745
Arabidopsis thaliana
?
-
-
-
-
additional information
HPR3 prefers NADPH over NADH and converts glycerate to hydroxypyruvate, the purified recombinant HPR3 shows similar activity with hydroxypyruvate and glyoxylate
737745
Arabidopsis thaliana
?
-
-
-
-
additional information
the recombinant AtHPR1 prefers NADH over NADPH and hydroxypyruvate over glyoxylate. Isozyme AtHPR1 also converts glyoxylate to glycolate, albeit with much lower catalytic efficiency than for hydroxypyruvate
737745
Arabidopsis thaliana
?
-
-
-
-
additional information
the recombinant AtHPR2 prefers NADPH over NADH but utilizes hydroxypyruvate and glyoxylate similarly
737745
Arabidopsis thaliana
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the beta-HAD (beta-hydroxyacid dehydrogenase) protein family. AtHPR2 and AtHPR3 are 45% identical to each other at the amino acid level, but only 19-25% identical to AtHPR1, the NADH-dependent form, and 8-9% identical to the AtGLYRs. None of the AtHPRs contains the active-site residues conserved in AtGLYR1 and AtGLYR2, indicating that the sites responsible for reducing glyoxylate differ greatly between the AtGLYRs and AtHPRs; the enzyme belongs to the beta-HAD (beta-hydroxyacid dehydrogenase) protein family. AtHPR2 and AtHPR3 are 45% identical to each other at the amino acid level, but only 19-25% identical to AtHPR1, the NADH-dependent form, and 8-9% identical to the AtGLYRs. None of the AtHPRs contains the active-site residues conserved in AtGLYR1 and AtGLYR2, indicating that the sites responsible for reducing glyoxylate differ greatly between the AtGLYRs and AtHPRs; the enzyme belongs to the beta-HAD (beta-hydroxyacid dehydrogenase) protein family. AtHPR2 and AtHPR3 are 45% identical to each other at the amino acid level, but only 19-25% identical to AtHPR1, the NADH-dependent form, and 8-9% identical to the AtGLYRs. None of the AtHPRs contains the active-site residues conserved in AtGLYR1 and AtGLYR2, indicating that the sites responsible for reducing glyoxylate differ greatly between the AtGLYRs and AtHPRs
Arabidopsis thaliana
physiological function
hydroxypyruvate reductase activity is important in the recycling of metabolites derived during photorespiration; hydroxypyruvate reductase activity is important in the recycling of metabolites derived during photorespiration; hydroxypyruvate reductase activity is important in the recycling of metabolites derived during photorespiration
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the beta-HAD (beta-hydroxyacid dehydrogenase) protein family. AtHPR2 and AtHPR3 are 45% identical to each other at the amino acid level, but only 19-25% identical to AtHPR1, the NADH-dependent form, and 8-9% identical to the AtGLYRs. None of the AtHPRs contains the active-site residues conserved in AtGLYR1 and AtGLYR2, indicating that the sites responsible for reducing glyoxylate differ greatly between the AtGLYRs and AtHPRs
Arabidopsis thaliana
physiological function
hydroxypyruvate reductase activity is important in the recycling of metabolites derived during photorespiration
Arabidopsis thaliana
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.232
-
Hydroxypyruvate
GLYR1, at pH 7.8 and 22C
Arabidopsis thaliana
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.232
-
Hydroxypyruvate
GLYR1, at pH 7.8 and 22C
Arabidopsis thaliana
Other publictions for EC 1.1.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739674
Lassalle
New insights into the mechanis ...
Pyrococcus furiosus, Pyrococcus horikoshii, Pyrococcus yayanosii, Pyrococcus yayanosii CH1
Sci. Rep.
6
20629
2016
-
-
3
3
-
-
3
6
-
-
-
8
-
4
-
-
3
-
-
-
-
-
8
-
-
-
-
6
-
-
-
6
4
-
-
-
-
3
6
3
-
-
-
3
4
6
-
-
-
8
-
-
-
3
-
-
-
-
8
-
-
-
-
6
-
-
-
-
-
-
-
-
6
6
737745
Hoover
Identification of catalyticall ...
Arabidopsis thaliana
Biochim. Biophys. Acta
1834
2663-2671
2013
-
-
1
-
-
-
-
1
4
-
-
6
-
5
-
-
-
-
-
-
-
-
14
-
-
-
-
-
1
-
-
5
-
-
-
-
-
1
13
-
-
-
-
-
-
1
4
-
-
6
-
-
-
-
-
-
-
-
14
-
-
-
-
-
1
-
-
-
-
2
6
-
1
1
726219
Timm
The hydroxypyruvate-reducing s ...
Arabidopsis thaliana
Plant Physiol.
155
694-705
2011
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
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-
1
2
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1
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-
2
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-
-
-
-
-
-
-
2
-
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-
-
-
-
-
-
-
-
1
1
-
-
-
695413
Piotrowska
-
Cellular response of light/dar ...
Chlorella vulgaris
Acta Physiol. Plant.
31
573-585
2009
5
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
1
-
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5
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
705820
Wang
Shoot-specific down-regulation ...
Brassica napus
Mol. Plant
2
191-200
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
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1
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1
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1
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1
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1
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700736
Timm
A cytosolic pathway for the co ...
Arabidopsis thaliana
Plant Cell
20
2848-2859
2008
-
-
1
-
1
-
1
-
2
-
1
1
-
5
-
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1
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3
1
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2
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2
4
-
2
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2
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2
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1
1
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2
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3
1
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-
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1
1
-
-
-
684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
-
-
1
1
-
-
-
4
-
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2
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1
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1
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4
1
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4
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2
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1
2
1
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4
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2
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1
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4
1
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4
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688165
Shen
Over-expression of a hydroxypy ...
Methylobacterium sp., Methylobacterium sp. MB200
J. Ind. Microbiol. Biotechnol.
34
657-663
2007
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1
1
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1
2
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4
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1
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4
1
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1
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1
1
1
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1
2
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1
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4
1
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-
-
-
-
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669886
Booth
Structural basis of substrate ...
Homo sapiens, Mus musculus
J. Mol. Biol.
360
178-189
2006
-
-
2
2
8
-
1
-
1
-
-
6
-
2
-
-
2
-
-
3
-
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8
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1
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4
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2
4
2
8
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1
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1
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6
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2
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3
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8
-
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1
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286510
Hagishita
Immunological characterization ...
Cucumis sativus, Hyphomicrobium methylovorum GM2, Methylobacterium extorquens AM1, Methylobacterium organophilum, Spinacia oleracea
FEMS Microbiol. Lett.
142
49-52
1996
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6
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9
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6
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5
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8
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287364
Yoshida
Cloning and expression of the ...
Hyphomicrobium methylovorum GM2
Eur. J. Biochem.
223
727-732
1994
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1
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4
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1
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7
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287365
Oliver
Inhibition of tobacco NADH-hyd ...
Nicotiana tabacum cv. SR1
Mol. Gen. Genet.
239
425-434
1993
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1
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286505
Kleczkowski
Oxalate as a potent and select ...
Spinacia oleracea
Biochem. J.
276
125-127
1991
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3
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286508
Chistoserdova
Purification and characterizat ...
Methylobacterium extorquens AM1
J. Bacteriol.
173
7228-7232
1991
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9
5
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3
1
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287368
Kleczkowski
Enzymology of the reduction of ...
Hordeum vulgare
Plant Physiol.
94
819-825
1990
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2
2
1
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4
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287369
Krema
-
Hydroxypyruvate reductase from ...
Methylobacterium extorquens AM1
Methods Enzymol.
188
373-378
1990
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2
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1
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1
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287370
Kleczkowski
Identification of hydroxypyruv ...
Zea mays
Plant Physiol.
91
278-288
1989
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1
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287371
Murray
Metabolism of hydroxypyruvate ...
Hordeum vulgare
Plant Physiol.
91
395-400
1989
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287372
Sautter
Import of peroxisomal hydroxyp ...
Citrullus lanatus subsp. vulgaris, Cucurbita pepo, Spinacia oleracea
Planta
176
149-158
1988
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3
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8
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4
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287373
Kleczkowski
Subcellular location of NADPH- ...
Pisum sativum, Spinacia oleracea
Plant Physiol.
88
1182-1185
1988
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2
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2
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7
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287374
Kleczkowski
Purification and characterizat ...
Spinacia oleracea
Biochem. J.
250
145-152
1988
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5
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1
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1
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287383
Coderch
Effect of salts on D-glycerate ...
Bos taurus
Biochim. Biophys. Acta
566
21-31
1979
3
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4
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287375
Bamforth
-
Hydroxypyruvate reductase acti ...
Paracoccus denitrificans
J. Gen. Microbiol.
101
259-267
1977
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1
3
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6
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1
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287376
Feld
Comparison of D-glycerate dehy ...
Bos taurus, Sus scrofa
Arch. Biochem. Biophys.
166
417-425
1975
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5
3
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287377
Feld
D-Glycerate dehydrogenase from ...
Sus scrofa
Methods Enzymol.
41B
289-293
1975
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1
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2
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287378
Sugimoto
Mechanisms of inhibition and a ...
Bos taurus
J. Biochem.
72
1317-1325
1972
6
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6
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287379
Sugimoto
Purification and properties of ...
Bos taurus
J. Biochem.
72
1307-1315
1972
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1
9
4
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1
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2
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287380
Uhr
Glycine and serine inhibition ...
Rattus norvegicus
FEBS Lett.
17
137-140
1971
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2
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287381
Kohn
Tartaric acid metabolism. VII. ...
Delftia acidovorans
J. Biol. Chem.
243
2494-2499
1968
5
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1
1
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287382
Kohn
-
Hydroxypyruvate reductase (D-g ...
Delftia acidovorans
Methods Enzymol.
9
229-232
1966
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2
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1
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1
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1
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