BRENDA - Enzyme Database show
show all sequences of 1.1.1.81

The hydroxypyruvate-reducing system in Arabidopsis: multiple enzymes for the same end

Timm, S.; Florian, A.; Jahnke, K.; Nunes-Nesi, A.; Fernie, A.R.; Bauwe, H.; Plant Physiol. 155, 694-705 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NADPH + H+
Arabidopsis thaliana
-
glycolate + NADP+
-
-
?
hydroxypyruvate + NADPH
Arabidopsis thaliana
-
D-glycerate + NADP+
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9LE33
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADPH + H+
-
726219
Arabidopsis thaliana
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NADPH
-
726219
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADPH
NADPH is preferred over NADH
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Arabidopsis thaliana
NADPH
NADPH is preferred over NADH
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NADPH + H+
Arabidopsis thaliana
-
glycolate + NADP+
-
-
?
hydroxypyruvate + NADPH
Arabidopsis thaliana
-
D-glycerate + NADP+
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
glyoxylate + NADPH + H+
-
726219
Arabidopsis thaliana
glycolate + NADP+
-
-
-
?
hydroxypyruvate + NADPH
-
726219
Arabidopsis thaliana
D-glycerate + NADP+
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
deletion of isoform HPR3 results in slightly altered leaf concentrations of the photorespiratory intermediates HP, glycerate, and glycine, indicating a disrupted photorespiratory flux, but not in visible alteration of the phenotype.The combined deletion of of isoforms HPR1, HPR2, and HPR3 causes increased growth retardation, decreased photochemical efficiency, and reduced oxygen-dependent gas exchange in comparison with the hpr1hpr2 double mutant. Isoform HPR3 could provide a compensatory bypass for the reduction of hydroxypyruvate and glyoxylate within the chloroplast
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
deletion of isoform HPR3 results in slightly altered leaf concentrations of the photorespiratory intermediates HP, glycerate, and glycine, indicating a disrupted photorespiratory flux, but not in visible alteration of the phenotype.The combined deletion of of isoforms HPR1, HPR2, and HPR3 causes increased growth retardation, decreased photochemical efficiency, and reduced oxygen-dependent gas exchange in comparison with the hpr1hpr2 double mutant. Isoform HPR3 could provide a compensatory bypass for the reduction of hydroxypyruvate and glyoxylate within the chloroplast
Arabidopsis thaliana
Other publictions for EC 1.1.1.81
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Zong
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Identification of catalyticall ...
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1
1
726219
Timm
The hydroxypyruvate-reducing s ...
Arabidopsis thaliana
Plant Physiol.
155
694-705
2011
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1
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1
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2
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1
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1
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695413
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5
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1
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1
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2
2
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705820
Wang
Shoot-specific down-regulation ...
Brassica napus
Mol. Plant
2
191-200
2009
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5
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700736
Timm
A cytosolic pathway for the co ...
Arabidopsis thaliana
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6
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1
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3
1
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684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
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1
1
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1
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4
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34
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2007
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1
1
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1
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1
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4
1
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1
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669886
Booth
Structural basis of substrate ...
Homo sapiens, Mus musculus
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2
2
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2
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3
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8
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286510
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Yoshida
Cloning and expression of the ...
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1994
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1
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4
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287365
Oliver
Inhibition of tobacco NADH-hyd ...
Nicotiana tabacum cv. SR1
Mol. Gen. Genet.
239
425-434
1993
-
-
-
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1
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1
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286505
Kleczkowski
Oxalate as a potent and select ...
Spinacia oleracea
Biochem. J.
276
125-127
1991
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3
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1
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1
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286508
Chistoserdova
Purification and characterizat ...
Methylobacterium extorquens AM1
J. Bacteriol.
173
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1991
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5
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287368
Kleczkowski
Enzymology of the reduction of ...
Hordeum vulgare
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1990
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2
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287369
Krema
-
Hydroxypyruvate reductase from ...
Methylobacterium extorquens AM1
Methods Enzymol.
188
373-378
1990
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-
-
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2
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1
1
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1
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1
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287370
Kleczkowski
Identification of hydroxypyruv ...
Zea mays
Plant Physiol.
91
278-288
1989
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1
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287371
Murray
Metabolism of hydroxypyruvate ...
Hordeum vulgare
Plant Physiol.
91
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1989
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287372
Sautter
Import of peroxisomal hydroxyp ...
Citrullus lanatus subsp. vulgaris, Cucurbita pepo, Spinacia oleracea
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1988
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287373
Kleczkowski
Subcellular location of NADPH- ...
Pisum sativum, Spinacia oleracea
Plant Physiol.
88
1182-1185
1988
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2
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2
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7
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1
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3
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1
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287374
Kleczkowski
Purification and characterizat ...
Spinacia oleracea
Biochem. J.
250
145-152
1988
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1
1
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2
1
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5
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2
1
1
3
1
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1
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2
1
1
3
1
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1
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287383
Coderch
Effect of salts on D-glycerate ...
Bos taurus
Biochim. Biophys. Acta
566
21-31
1979
3
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1
4
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Kohn
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