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Literature summary for 1.1.1.81 extracted from

  • Bamforth, C.W.; Quayle, J.R.
    Hydroxypyruvate reductase activity in Paracoccus denitrificans (1977), J. Gen. Microbiol., 101, 259-267.
No PubMed abstract available
Search for more literature for 1.1.1.81

General Stability

General Stability Organism
activity is slowly lost on storage at -15°C interspersed with frequent thawing and re-freezing, about 20% activity is lost over 8 cycles of freezing and thawing Paracoccus denitrificans

Inhibitors

Inhibitors Comment Organism Structure
bromide 33 mM, 38% inhibition Paracoccus denitrificans
citrate competitive vs. hydroxypyruvate Paracoccus denitrificans
Iodide 33 mM, 39% inhibition Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxypyruvate + NAD(P)H Paracoccus denitrificans
-
 D-glycerate + NAD(P)+ + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.077
-
 oxidation of glycerate Paracoccus denitrificans
0.135
-
 cofactor NADH Paracoccus denitrificans
0.33
-
 cofactor NADPH Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-butandione + NAD(P)H 33 mM, 12% of activity with hydroxypyruvate Paracoccus denitrificans butan-2-ol-3-one + NAD(P)+
-
 ?
acetoin + NAD(P)H + H+ 33 mM, 14% of activity with hydroxypyruvate Paracoccus denitrificans 2,3-butanediol + NAD(P)+
-
 ?
glyoxylate + NAD(P)H 33 mM, 15% activity Paracoccus denitrificans glycolate + NAD(P)+
-
 ?
hydroxypyruvate + NAD(P)H
-
 Paracoccus denitrificans D-glycerate + NAD(P)+ + H+
-
 ?
hydroxypyruvate + NAD(P)H
-
 Paracoccus denitrificans D-glycerate + NAD(P)+ + H+
-
 r
oxaloacetate + NAD(P)H 33 mM, 32% of activity with hydroxypyruvate Paracoccus denitrificans malate + NAD(P)+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 half-life of 35 min in 50 mM phosphate buffer, pH 7.0 Paracoccus denitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 sharp peak Paracoccus denitrificans