BRENDA - Enzyme Database show
show all sequences of 1.1.1.80

Secondary alcohol dehydrogenase catalyzes the reduction of exogenous acetone to 2-propanol in Trichomonas vaginalis

Sutak, R.; Hrdy, I.; Dolezal, P.; Cabala, R.; Sedinova, M.; Lewin, J.; Harant, K.; Mueller, M.; Tachezy, J.; FEBS J. 279, 2768-2780 (2012)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
5 mM, 80% residual activity
Trichomonas vaginalis
4-hydroxymercuribenzoic acid
0.1 mM, 49% residual activity
Trichomonas vaginalis
4-Methylpyrazole
5 mM, 68% residual activity
Trichomonas vaginalis
iodacetamide
50 mM, 49% residual activity
Trichomonas vaginalis
additional information
not inhibitory: EDTA up to 5 mM
Trichomonas vaginalis
pyrazole
5 mM, 74% residual activity
Trichomonas vaginalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000061
-
Butanone
37°C, pH 7.0
Trichomonas vaginalis
0.000093
-
acetone
37°C, pH 7.0
Trichomonas vaginalis
0.000256
-
pentan-3-one
37°C, pH 7.0
Trichomonas vaginalis
0.00028
-
acetaldehyde
37°C, pH 7.0
Trichomonas vaginalis
0.00056
-
propan-2-ol
37°C, pH 9.0
Trichomonas vaginalis
0.00078
-
butan-2-ol
37°C, pH 9.0
Trichomonas vaginalis
0.0015
-
pentan-3-ol
37°C, pH 9.0
Trichomonas vaginalis
0.0052
-
NADP+
pH 7.0, 37°C, cosubstrate acetone
Trichomonas vaginalis
0.0056
-
pentan-2-ol
37°C, pH 9.0
Trichomonas vaginalis
0.0275
-
propan-1-ol
37°C, pH 9.0
Trichomonas vaginalis
0.1
-
ethanol
37°C, pH 9.0
Trichomonas vaginalis
0.51
-
NADPH
pH 9.0, 37°C, cosubstrate propan-2-ol
Trichomonas vaginalis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Trichomonas vaginalis
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39100
-
2 * 43000, SDS-PAGE, 2 * 39100, calculated
Trichomonas vaginalis
43000
-
2 * 43000, SDS-PAGE, 2 * 39100, calculated
Trichomonas vaginalis
81000
-
gel filtration
Trichomonas vaginalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butan-2-ol + NADP+
Trichomonas vaginalis
preferred direction of the reaction is reduction rather than oxidation
butanone + NADPH + H+
-
-
r
propan-2-ol + NADP+
Trichomonas vaginalis
preferred direction of the reaction is reduction rather than oxidation
acetone + NADPH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trichomonas vaginalis
A2DJ40
putative
-
Purification (Commentary)
Commentary
Organism
-
Trichomonas vaginalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
-
724969
Trichomonas vaginalis
ethanol + NADP+
-
-
-
r
acetone + NADPH + H+
-
724969
Trichomonas vaginalis
propan-2-ol + NADP+
-
-
-
r
butan-2-ol + NADP+
preferred direction of the reaction is reduction rather than oxidation
724969
Trichomonas vaginalis
butanone + NADPH + H+
-
-
-
r
butanone + NADPH + H+
-
724969
Trichomonas vaginalis
butan-2-ol + NADP+
-
-
-
r
ethanol + NADP+
-
724969
Trichomonas vaginalis
acetaldehyde + NADPH + H+
-
-
-
r
pentan-2-ol + NADP+
-
724969
Trichomonas vaginalis
pentan-2-one + NADPH + H+
-
-
-
r
pentan-3-ol + NADP+
-
724969
Trichomonas vaginalis
pentan-3-one + NADPH + H+
-
-
-
r
pentan-3-one + NADPH + H+
-
724969
Trichomonas vaginalis
pentan-3-ol + NADP+
-
-
-
r
propan-1-ol + NADP+
-
724969
Trichomonas vaginalis
propanal + NADPH + H+
-
-
-
r
propan-2-ol + NADP+
preferred direction of the reaction is reduction rather than oxidation
724969
Trichomonas vaginalis
acetone + NADPH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 43000, SDS-PAGE, 2 * 39100, calculated
Trichomonas vaginalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
reduction of acetone, citrate buffer
Trichomonas vaginalis
6.5
7.5
reduction of acetone, Bis/Tris propane buffer
Trichomonas vaginalis
8
8.5
oxidation of propan-2-ol, Bis/Tris propane buffer
Trichomonas vaginalis
9.5
-
oxidation of propan-2-ol, glycine/HCl buffer
Trichomonas vaginalis
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
strictly specific for NADPH
Trichomonas vaginalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
strictly specific for NADPH
Trichomonas vaginalis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
5 mM, 80% residual activity
Trichomonas vaginalis
4-hydroxymercuribenzoic acid
0.1 mM, 49% residual activity
Trichomonas vaginalis
4-Methylpyrazole
5 mM, 68% residual activity
Trichomonas vaginalis
iodacetamide
50 mM, 49% residual activity
Trichomonas vaginalis
additional information
not inhibitory: EDTA up to 5 mM
Trichomonas vaginalis
pyrazole
5 mM, 74% residual activity
Trichomonas vaginalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000061
-
Butanone
37°C, pH 7.0
Trichomonas vaginalis
0.000093
-
acetone
37°C, pH 7.0
Trichomonas vaginalis
0.000256
-
pentan-3-one
37°C, pH 7.0
Trichomonas vaginalis
0.00028
-
acetaldehyde
37°C, pH 7.0
Trichomonas vaginalis
0.00056
-
propan-2-ol
37°C, pH 9.0
Trichomonas vaginalis
0.00078
-
butan-2-ol
37°C, pH 9.0
Trichomonas vaginalis
0.0015
-
pentan-3-ol
37°C, pH 9.0
Trichomonas vaginalis
0.0052
-
NADP+
pH 7.0, 37°C, cosubstrate acetone
Trichomonas vaginalis
0.0056
-
pentan-2-ol
37°C, pH 9.0
Trichomonas vaginalis
0.0275
-
propan-1-ol
37°C, pH 9.0
Trichomonas vaginalis
0.1
-
ethanol
37°C, pH 9.0
Trichomonas vaginalis
0.51
-
NADPH
pH 9.0, 37°C, cosubstrate propan-2-ol
Trichomonas vaginalis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Trichomonas vaginalis
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39100
-
2 * 43000, SDS-PAGE, 2 * 39100, calculated
Trichomonas vaginalis
43000
-
2 * 43000, SDS-PAGE, 2 * 39100, calculated
Trichomonas vaginalis
81000
-
gel filtration
Trichomonas vaginalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
butan-2-ol + NADP+
Trichomonas vaginalis
preferred direction of the reaction is reduction rather than oxidation
butanone + NADPH + H+
-
-
r
propan-2-ol + NADP+
Trichomonas vaginalis
preferred direction of the reaction is reduction rather than oxidation
acetone + NADPH + H+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Trichomonas vaginalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADPH + H+
-
724969
Trichomonas vaginalis
ethanol + NADP+
-
-
-
r
acetone + NADPH + H+
-
724969
Trichomonas vaginalis
propan-2-ol + NADP+
-
-
-
r
butan-2-ol + NADP+
preferred direction of the reaction is reduction rather than oxidation
724969
Trichomonas vaginalis
butanone + NADPH + H+
-
-
-
r
butanone + NADPH + H+
-
724969
Trichomonas vaginalis
butan-2-ol + NADP+
-
-
-
r
ethanol + NADP+
-
724969
Trichomonas vaginalis
acetaldehyde + NADPH + H+
-
-
-
r
pentan-2-ol + NADP+
-
724969
Trichomonas vaginalis
pentan-2-one + NADPH + H+
-
-
-
r
pentan-3-ol + NADP+
-
724969
Trichomonas vaginalis
pentan-3-one + NADPH + H+
-
-
-
r
pentan-3-one + NADPH + H+
-
724969
Trichomonas vaginalis
pentan-3-ol + NADP+
-
-
-
r
propan-1-ol + NADP+
-
724969
Trichomonas vaginalis
propanal + NADPH + H+
-
-
-
r
propan-2-ol + NADP+
preferred direction of the reaction is reduction rather than oxidation
724969
Trichomonas vaginalis
acetone + NADPH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 43000, SDS-PAGE, 2 * 39100, calculated
Trichomonas vaginalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
reduction of acetone, citrate buffer
Trichomonas vaginalis
6.5
7.5
reduction of acetone, Bis/Tris propane buffer
Trichomonas vaginalis
8
8.5
oxidation of propan-2-ol, Bis/Tris propane buffer
Trichomonas vaginalis
9.5
-
oxidation of propan-2-ol, glycine/HCl buffer
Trichomonas vaginalis
Expression
Organism
Commentary
Expression
Trichomonas vaginalis
iron limitation results in a 1.5fold increase in enzyme mRNA
up
Expression (protein specific)
Organism
Commentary
Expression
Trichomonas vaginalis
iron limitation results in a 1.5fold increase in enzyme mRNA
up
Other publictions for EC 1.1.1.80
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724969
Sutak
Secondary alcohol dehydrogenas ...
Trichomonas vaginalis
FEBS J.
279
2768-2780
2012
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12
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3
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4
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10
1
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4
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1
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6
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12
1
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3
2
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1
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10
1
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4
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1
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1
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736276
Peretz
Stereospecificity of hydrogen ...
Thermoanaerobacter brockii
Int. J. Pept. Protein Res.
42
490-495
1993
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1
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1
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287362
Ploc
Gas chromatographic study of t ...
Rattus norvegicus
J. Chromatogr.
172
374-378
1979
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1
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4
2
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1
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1
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1
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10
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2
1
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2
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1
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2
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4
2
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1
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-
1
-
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10
-
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2
1
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