General Stability | Organism |
---|---|
dithiothreitol stabilizes | Drosophila melanogaster |
phenylmethane-sulfonyl fluoride stabilizes during purification | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | weak inhibitor of both isozymes, i.e. GPDH-1 and -3 | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE, confirmed by amino acid composition | Drosophila melanogaster |
66000 | - |
gel filtration | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Drosophila melanogaster | - |
dihydroxyacetone phosphate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3 isozymes in adults and 1 in larvae, distinct in isoelectric focusing | Drosophila melanogaster |
affinity chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | late third instar | Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
isozyme GPDH-3 | Drosophila melanogaster |
179.8 | - |
isozyme GPDH-1 | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Drosophila melanogaster | dihydroxyacetone phosphate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE, confirmed by amino acid composition | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Drosophila melanogaster | |
NADH | - |
Drosophila melanogaster |