BRENDA - Enzyme Database show
show all sequences of 1.1.1.79

Purification and some properties of glyoxylate reductase (NADP+) and its functional location in mitochondria in Euglena gracilis Z

Yokota, A.; Haga, S.; Kitaoka, S.; Biochem. J. 227, 211-216 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
adenine
0.01 mM, 70% inhibition after preincubation for 5 min
Euglena gracilis
ATP
10 mM, 20% inhibition
Euglena gracilis
N-ethylmaleimide
10.0 mM, 75% inhibition after preincubation for 5 min
Euglena gracilis
p-chloromercuribenzoate
0.1 mM, 55% inhibition after preincubation for 5 min
Euglena gracilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
NADPH
-
Euglena gracilis
0.045
-
glyoxylate
-
Euglena gracilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
intermembrane space and inner membrane
Euglena gracilis
5739
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
82000
-
gel filtration
Euglena gracilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NAD(P)H
Euglena gracilis
specific for NADPH
glycolate + NAD(P)+
-
Euglena gracilis
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Euglena gracilis
-
-
-
Purification (Commentary)
Commentary
Organism
ethanol precipitation, DEAE-cellulose, CM-cellulose, affinity chromatography
Euglena gracilis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
17.1
-
-
Euglena gracilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + NADH + H+
13.9% of glyoxylate activity
287321
Euglena gracilis
2-hydroxyglutarate + NAD+
-
287321
Euglena gracilis
?
acetaldehyde + NADPH + H+
10.4% of glyoxylate activity
287321
Euglena gracilis
ethanol + NADP+
-
287321
Euglena gracilis
?
glyoxylate + NAD(P)H
specific for NADPH
287321
Euglena gracilis
glycolate + NAD(P)+
-
287321
Euglena gracilis
?
oxaloacetate + NADPH
28.6% of glyoxylate activity
287321
Euglena gracilis
malate + NADP+
-
287321
Euglena gracilis
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.45
-
enzyme activity decreases by 40% and 50% at pH 5.5 and 8.0 respectively
Euglena gracilis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
specific for NADPH
Euglena gracilis
NADPH
specific for NADPH
Euglena gracilis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
specific for NADPH
Euglena gracilis
NADPH
specific for NADPH
Euglena gracilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
adenine
0.01 mM, 70% inhibition after preincubation for 5 min
Euglena gracilis
ATP
10 mM, 20% inhibition
Euglena gracilis
N-ethylmaleimide
10.0 mM, 75% inhibition after preincubation for 5 min
Euglena gracilis
p-chloromercuribenzoate
0.1 mM, 55% inhibition after preincubation for 5 min
Euglena gracilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0039
-
NADPH
-
Euglena gracilis
0.045
-
glyoxylate
-
Euglena gracilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
intermembrane space and inner membrane
Euglena gracilis
5739
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
82000
-
gel filtration
Euglena gracilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
glyoxylate + NAD(P)H
Euglena gracilis
specific for NADPH
glycolate + NAD(P)+
-
Euglena gracilis
?
Purification (Commentary) (protein specific)
Commentary
Organism
ethanol precipitation, DEAE-cellulose, CM-cellulose, affinity chromatography
Euglena gracilis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
17.1
-
-
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-oxoglutarate + NADH + H+
13.9% of glyoxylate activity
287321
Euglena gracilis
2-hydroxyglutarate + NAD+
-
287321
Euglena gracilis
?
acetaldehyde + NADPH + H+
10.4% of glyoxylate activity
287321
Euglena gracilis
ethanol + NADP+
-
287321
Euglena gracilis
?
glyoxylate + NAD(P)H
specific for NADPH
287321
Euglena gracilis
glycolate + NAD(P)+
-
287321
Euglena gracilis
?
oxaloacetate + NADPH
28.6% of glyoxylate activity
287321
Euglena gracilis
malate + NADP+
-
287321
Euglena gracilis
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.45
-
enzyme activity decreases by 40% and 50% at pH 5.5 and 8.0 respectively
Euglena gracilis
Other publictions for EC 1.1.1.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739242
Zong
Up regulation of glyoxylate re ...
Homo sapiens
Pathol. Res. Pract.
212
365-371
2016
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1
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1
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1
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3
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1
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1
1
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722899
Ching
Glyoxylate reductase isoform 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
54
152-168
2012
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1
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2
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2
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1
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2
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2
2
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696125
Allan
Role of plant glyoxylate reduc ...
Arabidopsis thaliana
Biochem. J.
423
15-22
2009
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1
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2
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685774
Ogino
Characterization of recombinan ...
Thermus thermophilus, Thermus thermophilus HB27
Biotechnol. Prog.
24
321-325
2008
-
-
2
-
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-
3
8
-
-
1
2
10
2
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-
2
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5
1
2
-
4
8
1
1
-
4
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2
4
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3
-
8
-
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1
2
10
-
-
2
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-
-
-
5
1
2
-
4
8
1
1
-
-
-
-
-
-
-
-
688090
Simpson
Identification and characteriz ...
Arabidopsis thaliana
J. Exp. Bot.
59
2545-2554
2008
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1
-
2
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1
4
2
-
4
4
-
7
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1
-
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
2
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-
2
4
-
2
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-
1
-
4
2
-
4
4
-
-
-
1
-
1
-
4
15
1
-
-
-
4
1
1
-
2
-
-
-
-
-
-
688091
Allan
Gamma-hydroxybutyrate accumula ...
Arabidopsis thaliana, Nicotiana tabacum
J. Exp. Bot.
59
2555-2564
2008
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2
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2
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5
-
5
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7
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2
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2
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2
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5
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7
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684141
Yoshikawa
Structure of archaeal glyoxyla ...
Pyrococcus horikoshii OT3
Acta Crystallogr. Sect. D
63
357-365
2007
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1
1
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4
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2
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1
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1
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1
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1
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4
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685902
Hoover
Kinetic mechanism of a recombi ...
Arabidopsis thaliana
Can. J. Bot.
85
896-902
2007
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1
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3
2
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1
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1
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1
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2
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690207
Rintala
The ORF YNL274c (GOR1) codes f ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Yeast
24
129-136
2007
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1
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1
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21
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3
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6
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1
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1
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669886
Booth
Structural basis of substrate ...
Homo sapiens
J. Mol. Biol.
360
178-189
2006
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1
1
4
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1
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1
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667814
Mdluli
A preliminary account of the p ...
Homo sapiens
Biochim. Biophys. Acta
1753
209-216
2005
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669351
Genolet
Promoter rearrangements cause ...
Homo sapiens, Mus musculus
J. Biol. Chem.
280
24143-24152
2005
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287318
Kleczkowski
-
Kinetics and regulation of the ...
Spinacia oleracea
Z. Naturforsch. C
50
21-28
1995
1
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4
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287319
Husic
NADH:hydroxypyruvate reductase ...
Chlamydomonas reinhardtii
Arch. Biochem. Biophys.
252
396-408
1987
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287320
Kleczkowski
Purification and characterizat ...
Glycine max, Spinacia oleracea, Triticum aestivum
Biochem. J.
239
653-659
1986
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287321
Yokota
Purification and some properti ...
Euglena gracilis
Biochem. J.
227
211-216
1985
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287322
Mulligan
Effects of glyoxylate on photo ...
Spinacia oleracea
Plant Physiol.
72
415-419
1983
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286409
Yokota
Occurrence and subcellular dis ...
Euglena gracilis
Agric. Biol. Chem.
45
15-22
1981
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287324
Fukuda
Purification and properties of ...
Neurospora crassa
Agric. Biol. Chem.
45
1153-1158
1981
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2
4
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1
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1
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287325
Fukuda
Purification and enzymatic pro ...
Saccharomyces cerevisiae
J. Biochem.
87
841-846
1980
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4
4
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1
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3
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1
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-
1
-
-
1
1
3
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287326
Sagisaka
Occurrence of nicotinamide ade ...
Populus gelrica
Plant Physiol.
65
377-381
1980
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287327
Yokota
Occurrence and properties of t ...
Euglena gracilis
Biochem. J.
184
189-192
1979
-
-
-
-
-
-
-
2
3
-
-
1
-
5
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
3
-
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287328
Tochikura
Purification and properties of ...
Saccharomyces cerevisiae
J. Biochem.
86
105-110
1979
-
-
-
-
-
-
10
2
-
-
1
1
-
1
-
-
1
-
-
-
1
1
5
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
10
-
2
-
-
1
1
-
-
-
1
-
-
1
1
5
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
287329
Zeltich
Effect of glycidate, an inhibi ...
Nicotiana tabacum
Plant Physiol.
61
236-241
1978
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-