BRENDA - Enzyme Database show
show all sequences of 1.1.1.78

Metabolism of 2-oxoaldehydes in bacteria: purification and characterization of methylglyoxal reductase from Escherichia coli

Saikusa, T.; Rhee, H.; Watanabe, K.; Murata, K.; Kimura, A.; Agric. Biol. Chem. 51, 1893-1899 (1987)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
slight activation
Escherichia coli
dithiothreitol
slight activation
Escherichia coli
glutathione
slight activation
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
-
Escherichia coli
Cu2+
-
Escherichia coli
Hg2+
-
Escherichia coli
iodoacetate
-
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Ni2+
-
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
Zn2+
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADH
Escherichia coli
-
(R)-lactaldehyde + NAD+
-
Escherichia coli
?
methylglyoxal + NADPH
Escherichia coli
-
lactaldehyde + NADP+
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.26
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + NADH
-
207976
Escherichia coli
(R)-lactaldehyde + NAD+
-
207976
Escherichia coli
?
methylglyoxal + NADPH
-
207976
Escherichia coli
lactaldehyde + NADP+
-
207976
Escherichia coli
?
additional information
enzyme utilizes both: NADH and NADPH
207976
Escherichia coli
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Escherichia coli
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
75
25C and 75C: about 50% of activity maximum
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
9.5
pH 4.5 and pH 9.5: about 50% of activity maximum
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
utilizes both NADPH and NADH
Escherichia coli
NADPH
utilizes both NADH and NADPH
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
slight activation
Escherichia coli
dithiothreitol
slight activation
Escherichia coli
glutathione
slight activation
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
utilizes both NADPH and NADH
Escherichia coli
NADPH
utilizes both NADH and NADPH
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
-
Escherichia coli
Cu2+
-
Escherichia coli
Hg2+
-
Escherichia coli
iodoacetate
-
Escherichia coli
N-ethylmaleimide
-
Escherichia coli
Ni2+
-
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
Zn2+
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43000
-
gel filtration, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADH
Escherichia coli
-
(R)-lactaldehyde + NAD+
-
Escherichia coli
?
methylglyoxal + NADPH
Escherichia coli
-
lactaldehyde + NADP+
-
Escherichia coli
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.26
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methylglyoxal + NADH
-
207976
Escherichia coli
(R)-lactaldehyde + NAD+
-
207976
Escherichia coli
?
methylglyoxal + NADPH
-
207976
Escherichia coli
lactaldehyde + NADP+
-
207976
Escherichia coli
?
additional information
enzyme utilizes both: NADH and NADPH
207976
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43000, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Escherichia coli
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
25
75
25C and 75C: about 50% of activity maximum
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
9.5
pH 4.5 and pH 9.5: about 50% of activity maximum
Escherichia coli
Other publictions for EC 1.1.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
657418
Chen
Associating protein activities ...
Saccharomyces cerevisiae
Yeast
20
545-554
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207975
Kato
-
Purification and partial chara ...
Sus scrofa
Agric. Biol. Chem.
52
2641-2642
1988
-
-
-
-
-
-
1
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
6
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207976
Saikusa
-
Metabolism of 2-oxoaldehydes i ...
Escherichia coli
Agric. Biol. Chem.
51
1893-1899
1987
3
-
-
-
-
-
8
1
-
-
1
2
-
1
-
-
1
-
-
-
1
-
3
1
1
1
-
-
1
1
-
2
-
-
-
3
-
-
2
-
-
-
-
8
-
1
-
-
1
2
-
-
-
1
-
-
1
-
3
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
137611
Tran-Din
-
Formation of D(-)-1,2-propaned ...
Clostridium sphenoides
Arch. Microbiol.
142
87-92
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207969
Ray
Purification and partial chara ...
Capra hircus
Biochim. Biophys. Acta
802
119-127
1984
-
-
-
-
-
-
2
3
1
-
2
1
-
2
-
-
-
-
-
2
1
1
5
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
3
1
-
2
1
-
-
-
-
-
2
1
1
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207968
Ting
The metabolism of lactaldehyde ...
Rattus norvegicus
Biochim. Biophys. Acta
97
407-415
1965
-
-
-
-
-
-
3
2
-
-
-
1
-
1
-
-
1
-
-
7
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
7
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-