BRENDA - Enzyme Database show
show all sequences of 1.1.1.78

Purification and partial characterization of NADH-dependent methylglyoxal-reducing enzyme from porcine liver

Kato, H.; Miyauchi, Y.; Nishimura, T.; Liang, Z.Q.; Agric. Biol. Chem. 52, 2641-2642 (1988)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
-
Sus scrofa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Sus scrofa
69000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADH
Sus scrofa
-
(R)-lactaldehyde + NAD+
-
Sus scrofa
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sus scrofa
-
-
-
Purification (Commentary)
Commentary
Organism
-
Sus scrofa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADH + H+
-
207975
Sus scrofa
ethanol + NAD+
-
207975
Sus scrofa
?
DL-glyceraldehyde + NAD+
-
207975
Sus scrofa
?
-
-
-
-
glutaraldehyde + NADH
-
207975
Sus scrofa
?
-
-
-
?
glyoxal + NADH
-
207975
Sus scrofa
glycolaldehyde + NAD+
-
207975
Sus scrofa
?
methylglyoxal + NADH
-
207975
Sus scrofa
(R)-lactaldehyde + NAD+
-
207975
Sus scrofa
?
phenylglyoxal + NADH
-
207975
Sus scrofa
hydroxyphenylacetaldehyde + NAD+
-
207975
Sus scrofa
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Sus scrofa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Sus scrofa
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Sus scrofa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Sus scrofa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
-
Sus scrofa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
2 * 35000, SDS-PAGE
Sus scrofa
69000
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADH
Sus scrofa
-
(R)-lactaldehyde + NAD+
-
Sus scrofa
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sus scrofa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADH + H+
-
207975
Sus scrofa
ethanol + NAD+
-
207975
Sus scrofa
?
DL-glyceraldehyde + NAD+
-
207975
Sus scrofa
?
-
-
-
-
glutaraldehyde + NADH
-
207975
Sus scrofa
?
-
-
-
?
glyoxal + NADH
-
207975
Sus scrofa
glycolaldehyde + NAD+
-
207975
Sus scrofa
?
methylglyoxal + NADH
-
207975
Sus scrofa
(R)-lactaldehyde + NAD+
-
207975
Sus scrofa
?
phenylglyoxal + NADH
-
207975
Sus scrofa
hydroxyphenylacetaldehyde + NAD+
-
207975
Sus scrofa
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 35000, SDS-PAGE
Sus scrofa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
-
Sus scrofa
Other publictions for EC 1.1.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
657418
Chen
Associating protein activities ...
Saccharomyces cerevisiae
Yeast
20
545-554
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207975
Kato
-
Purification and partial chara ...
Sus scrofa
Agric. Biol. Chem.
52
2641-2642
1988
-
-
-
-
-
-
1
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
6
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207976
Saikusa
-
Metabolism of 2-oxoaldehydes i ...
Escherichia coli
Agric. Biol. Chem.
51
1893-1899
1987
3
-
-
-
-
-
8
1
-
-
1
2
-
1
-
-
1
-
-
-
1
-
3
1
1
1
-
-
1
1
-
2
-
-
-
3
-
-
2
-
-
-
-
8
-
1
-
-
1
2
-
-
-
1
-
-
1
-
3
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
137611
Tran-Din
-
Formation of D(-)-1,2-propaned ...
Clostridium sphenoides
Arch. Microbiol.
142
87-92
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207969
Ray
Purification and partial chara ...
Capra hircus
Biochim. Biophys. Acta
802
119-127
1984
-
-
-
-
-
-
2
3
1
-
2
1
-
2
-
-
-
-
-
2
1
1
5
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
3
1
-
2
1
-
-
-
-
-
2
1
1
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207968
Ting
The metabolism of lactaldehyde ...
Rattus norvegicus
Biochim. Biophys. Acta
97
407-415
1965
-
-
-
-
-
-
3
2
-
-
-
1
-
1
-
-
1
-
-
7
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
7
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-