BRENDA - Enzyme Database show
show all sequences of 1.1.1.78

Purification and partial characterization of a methylglyoxal reductase from goat liver

Ray, M.; Ray, S.; Biochim. Biophys. Acta 802, 119-127 (1984)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Capra hircus
p-chloromercuribenzoate
-
Capra hircus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NADH
-
Capra hircus
0.3
-
Phenylglyoxal
-
Capra hircus
0.4
-
methylglyoxal
-
Capra hircus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Capra hircus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
2 * 46000, SDS-PAGE
Capra hircus
89000
-
gel filtration
Capra hircus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADH
Capra hircus
-
(R)-lactaldehyde + NAD+
-
Capra hircus
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Capra hircus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Capra hircus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
69.2
-
-
Capra hircus
Storage Stability
Storage Stability
Organism
4°C, 15 days
Capra hircus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADH + H+
-
207969
Capra hircus
ethanol + NAD+
-
-
-
?
glyceraldehyde + NADH
-
207969
Capra hircus
glycerol + NAD+
-
-
-
?
methylglyoxal + NADH
-
207969
Capra hircus
(R)-lactaldehyde + NAD+
-
207969
Capra hircus
ir
additional information
NADH better substrate than NADPH
207969
Capra hircus
?
-
-
-
-
phenylglyoxal + NADH
-
207969
Capra hircus
hydroxyphenylacetaldehyde + NAD+
-
207969
Capra hircus
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 46000, SDS-PAGE
Capra hircus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of methylglyoxal
Capra hircus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH better substrate than NADPH
Capra hircus
NADPH
NADH better substrate than NADPH
Capra hircus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH better substrate than NADPH
Capra hircus
NADPH
NADH better substrate than NADPH
Capra hircus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
-
Capra hircus
p-chloromercuribenzoate
-
Capra hircus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NADH
-
Capra hircus
0.3
-
Phenylglyoxal
-
Capra hircus
0.4
-
methylglyoxal
-
Capra hircus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Capra hircus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
2 * 46000, SDS-PAGE
Capra hircus
89000
-
gel filtration
Capra hircus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methylglyoxal + NADH
Capra hircus
-
(R)-lactaldehyde + NAD+
-
Capra hircus
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Capra hircus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
69.2
-
-
Capra hircus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 15 days
Capra hircus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + NADH + H+
-
207969
Capra hircus
ethanol + NAD+
-
-
-
?
glyceraldehyde + NADH
-
207969
Capra hircus
glycerol + NAD+
-
-
-
?
methylglyoxal + NADH
-
207969
Capra hircus
(R)-lactaldehyde + NAD+
-
207969
Capra hircus
ir
additional information
NADH better substrate than NADPH
207969
Capra hircus
?
-
-
-
-
phenylglyoxal + NADH
-
207969
Capra hircus
hydroxyphenylacetaldehyde + NAD+
-
207969
Capra hircus
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 46000, SDS-PAGE
Capra hircus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of methylglyoxal
Capra hircus
Other publictions for EC 1.1.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
657418
Chen
Associating protein activities ...
Saccharomyces cerevisiae
Yeast
20
545-554
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207975
Kato
-
Purification and partial chara ...
Sus scrofa
Agric. Biol. Chem.
52
2641-2642
1988
-
-
-
-
-
-
1
-
-
-
2
1
-
1
-
-
1
-
-
-
-
-
6
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207976
Saikusa
-
Metabolism of 2-oxoaldehydes i ...
Escherichia coli
Agric. Biol. Chem.
51
1893-1899
1987
3
-
-
-
-
-
8
1
-
-
1
2
-
1
-
-
1
-
-
-
1
-
3
1
1
1
-
-
1
1
-
2
-
-
-
3
-
-
2
-
-
-
-
8
-
1
-
-
1
2
-
-
-
1
-
-
1
-
3
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
137611
Tran-Din
-
Formation of D(-)-1,2-propaned ...
Clostridium sphenoides
Arch. Microbiol.
142
87-92
1985
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
207969
Ray
Purification and partial chara ...
Capra hircus
Biochim. Biophys. Acta
802
119-127
1984
-
-
-
-
-
-
2
3
1
-
2
1
-
2
-
-
-
-
-
2
1
1
5
1
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
2
-
3
1
-
2
1
-
-
-
-
-
2
1
1
5
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
207968
Ting
The metabolism of lactaldehyde ...
Rattus norvegicus
Biochim. Biophys. Acta
97
407-415
1965
-
-
-
-
-
-
3
2
-
-
-
1
-
1
-
-
1
-
-
7
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
7
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-