BRENDA - Enzyme Database show
show all sequences of 1.1.1.77

Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties and comparison with the fucose-induced enzyme

Boronat, A.; Aguilar,J.; J. Bacteriol. 140, 320-326 (1979)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
L-1,2-propanediol
above 25 mM: substrate inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
L-lactaldehyde
pH 7.0
Escherichia coli
1.25
-
L-1,2-propanediol
pH 9.5
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
2 * 39000, SDS-PAGE
Escherichia coli
76000
-
gel permeation chromatography on a calibrated column of Ultrogel AcA 34 under denaturating conditions
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-propane-1,2-diol + NAD+
Escherichia coli
-
(S)-lactaldehyde + NADH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
crude extract, ammonium sulfate precipitation, heat treatment, ultrogel AcA 34 fractionation, DEAE-Sephadex column chromatography, second ultrogel AcA 34 fractionation
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-propane-1,2-diol + NAD+
acts only on L-isomer
287312
Escherichia coli
(R)-lactaldehyde + NADH
-
-
-
?
(S)-propane-1,2-diol + NAD+
-
287312
Escherichia coli
(S)-lactaldehyde + NADH
-
-
-
r
(S)-propane-1,2-diol + NAD+
only L-propane-1,2-diol is oxidized
287312
Escherichia coli
(S)-lactaldehyde + NADH
-
-
-
-
ethanol + NAD+
-
287312
Escherichia coli
acetaldehyde + NADH
-
-
-
r
ethylene glycol + NAD+
-
287312
Escherichia coli
glycolaldehyde + NAD+
-
-
-
r
glycerol + NAD+
-
287312
Escherichia coli
DL-glyceraldehyde + NADH
-
-
-
r
propanol + NAD+
-
287312
Escherichia coli
propionaldehyde + NADH
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 39000, SDS-PAGE
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of L-lactaldehyde
Escherichia coli
9.5
-
dehydrogenation of L-1,2-propanediol
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
10
reduction of lactaldehyde, pH 4.5: 50% of reductase activity maximum, pH 10: 10% of reductase activity maximum
Escherichia coli
8
11
dehydrogenation of 1,2-propanediol, pH 8: 10% of dehydrogenase activity maximum, pH 11: 65% of dehydrogenase activity maximum
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
L-1,2-propanediol
above 25 mM: substrate inhibition
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
L-lactaldehyde
pH 7.0
Escherichia coli
1.25
-
L-1,2-propanediol
pH 9.5
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
2 * 39000, SDS-PAGE
Escherichia coli
76000
-
gel permeation chromatography on a calibrated column of Ultrogel AcA 34 under denaturating conditions
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-propane-1,2-diol + NAD+
Escherichia coli
-
(S)-lactaldehyde + NADH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
crude extract, ammonium sulfate precipitation, heat treatment, ultrogel AcA 34 fractionation, DEAE-Sephadex column chromatography, second ultrogel AcA 34 fractionation
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-propane-1,2-diol + NAD+
acts only on L-isomer
287312
Escherichia coli
(R)-lactaldehyde + NADH
-
-
-
?
(S)-propane-1,2-diol + NAD+
-
287312
Escherichia coli
(S)-lactaldehyde + NADH
-
-
-
r
(S)-propane-1,2-diol + NAD+
only L-propane-1,2-diol is oxidized
287312
Escherichia coli
(S)-lactaldehyde + NADH
-
-
-
-
ethanol + NAD+
-
287312
Escherichia coli
acetaldehyde + NADH
-
-
-
r
ethylene glycol + NAD+
-
287312
Escherichia coli
glycolaldehyde + NAD+
-
-
-
r
glycerol + NAD+
-
287312
Escherichia coli
DL-glyceraldehyde + NADH
-
-
-
r
propanol + NAD+
-
287312
Escherichia coli
propionaldehyde + NADH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 39000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
reduction of L-lactaldehyde
Escherichia coli
9.5
-
dehydrogenation of L-1,2-propanediol
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
10
reduction of lactaldehyde, pH 4.5: 50% of reductase activity maximum, pH 10: 10% of reductase activity maximum
Escherichia coli
8
11
dehydrogenation of 1,2-propanediol, pH 8: 10% of dehydrogenase activity maximum, pH 11: 65% of dehydrogenase activity maximum
Escherichia coli
Other publictions for EC 1.1.1.77
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701174
Patel
Rhamnose catabolism in Bactero ...
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI-5482
Res. Microbiol.
159
678-684
2008
-
-
1
-
1
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-
2
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5
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2
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1
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1
1
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1
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2
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2
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-
287317
Cabiscol
Inactivation of propanediol ox ...
Escherichia coli
Biochim. Biophys. Acta
1118
155-160
1992
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-
-
-
-
-
4
-
-
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-
-
2
-
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1
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1
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1
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4
-
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-
1
-
-
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-
-
-
-
-
-
137611
Tran-Din
-
Formation of D(-)-1,2-propaned ...
Clostridium sphenoides
Arch. Microbiol.
142
87-92
1985
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-
-
-
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1
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1
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2
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1
1
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-
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-
-
1
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-
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1
-
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-
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1
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-
2
-
1
1
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-
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-
-
-
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-
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-
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-
287310
Weimer
Fermentation of 6-deoxyhexoses ...
Paenibacillus macerans
Appl. Environ. Microbiol.
47
263-267
1984
-
1
-
-
-
-
-
-
-
-
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1
-
2
-
-
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-
-
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3
-
1
1
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-
-
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-
2
-
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1
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2
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1
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-
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-
-
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3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
287316
Boronat
Metabolism of L-fucose and L-r ...
Escherichia coli
J. Bacteriol.
147
181-185
1981
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
1
-
-
-
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-
1
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1
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-
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1
-
-
-
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-
-
-
2
-
1
1
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-
287309
Kawagishi
Purification of NAD-dependent ...
Microcyclus eburneus
Agric. Biol. Chem.
44
949-950
1980
-
-
-
-
-
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-
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2
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1
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1
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1
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2
1
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1
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1
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2
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1
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1
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2
1
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-
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-
-
-
-
-
-
-
287312
Boronat
Rhamnose-induced propanediol o ...
Escherichia coli
J. Bacteriol.
140
320-326
1979
-
-
-
-
-
-
1
2
-
-
2
1
-
2
-
-
1
-
-
-
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-
7
1
-
-
-
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
2
1
-
-
-
1
-
-
-
-
7
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287314
Hacking
Evolution of propanediol utili ...
Escherichia coli
J. Bacteriol.
136
522-530
1978
-
-
-
-
-
-
-
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-
1
-
2
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2
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1
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1
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1
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2
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-
287313
Hacking
Disruption of the fucose pathw ...
Escherichia coli
J. Bacteriol.
126
1166-1172
1976
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2
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2
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3
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1
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1
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2
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3
-
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287315
Cocks
Evolution of L-1,2-propanediol ...
Escherichia coli
J. Bacteriol.
118
83-88
1974
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1
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1
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1
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1
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1
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287308
Ting
The metabolism of lactaldehyde ...
Rattus norvegicus
Biochim. Biophys. Acta
89
217-225
1965
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3
4
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1
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1
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6
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3
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1
1
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3
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3
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3
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4
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1
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6
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3
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1
1
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